- Identification and Quantification in Food
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Computational Drug Discovery Methods
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Viral gastroenteritis research and epidemiology
- Plant Virus Research Studies
- Food Safety and Hygiene
- Antibiotic Resistance in Bacteria
- Microbial Metabolism and Applications
- Probiotics and Fermented Foods
- Escherichia coli research studies
- Animal Diversity and Health Studies
- Machine Learning in Bioinformatics
- Urinary Tract Infections Management
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Fish Biology and Ecology Studies
- Vibrio bacteria research studies
- Helicobacter pylori-related gastroenterology studies
- Plant and Fungal Interactions Research
- Herpesvirus Infections and Treatments
- Veterinary medicine and infectious diseases
Ain Shams University
2024-2025
Taibah University
2023-2024
Suez Canal University
2013-2022
Abstract Background Bacillus cereus is implicated in severe foodborne infection humans. This study intended to assess the occurrence, gro EL gene sequencing, biofilm production, and resistance profiles of emerged multidrug resistant (MDR) B. meat product samples. Moreover, this work highlights virulence toxigenic genes ( hbl ABCD complex, nhe ABC cyt K, ces , pc-plc ) antimicrobial bla 1, tet A, 2, B, erm A). Methods Consequently, 200 samples (sausage, minced meat, luncheon, beef liver; n =...
Bovine mastitis caused by Staphylococcus aureus is considered a public health threat globally. Herein, we aimed to investigate the occurrence, agr typing, antimicrobial resistance patterns, biofilm production, and PCR-based detection of virulence, biofilm, adhesion, enterotoxins genes S. strains recovered from clinical subclinical bovine mastitis. The prevalence in examined milk samples was 44.4%. Besides, 95% retrieved were identified as MRSA. all tested isolates producers. PCR revealed...
This study aimed to investigate the prevalence of different bacterial species affecting ducks as well demonstrating antimicrobial susceptibility and molecular typing isolated strains.A total 500 samples were randomly collected from duck farms at Ismailia Governorate, Egypt. The subjected bacteriological examination. Polymerase chain reaction (PCR) was applied for amplification Kmt1 gene Pasteurella multocida X region protein-A (spA) Staphylococcus aureus strains ensure their virulence....
Urinary tract infection (UTI) is considered one of the most prevalent infections that may lead to many renal complications. They account for almost 10% all in Saudi Arabia, making them second common cause emergency department admissions. Bacterial pathogens, primarily Escherichia coli, Klebsiella spp., Enterococcus Proteus and Staphylococcus spp. are causative agents UTI. This study aims evaluate prevalence antimicrobial susceptibility patterns uropathogens adult patients from Madinah,...
Outbreaks of Monkeypox (mpox) in over 100 non-endemic countries 2022 represented a serious global health concern. Once neglected disease, mpox has become public issue. A42R profilin-like protein from (PDB ID: 4QWO) represents potential new lead for drug development and may interact with various synthetic natural compounds. In this report, the interaction six phytochemicals found medicinal plant Ficus religiosa (abundant India) was examined. Based on predicted compared protein-ligand binding...
Antibiotic resistance has been a progressively documented problem, resulting in treatment failure humans and animals. This study aimed to investigate the antimicrobial susceptibility virulence of extensively drug-resistant (XDR) Aeromonas spp. wild Mugil cephalus its surrounding seawater along coastal road Port Said, Egypt.Specimens were examined bacteriologically, confirmed biochemically, tested for their sensitivity against 11 agents. Molecular confirmation obtained isolates by 16S rRNA...
In response to the escalating threat of drug-resistant fungi human health, there is an urgent need for innovative strategies. Our focus on addressing this challenge by exploring a previously untapped target, yeast casein kinase (Yck2), as potential space antifungal development. To identify promising candidates, we conducted thorough screening diverse-lib drug-like molecule library, comprising 99,288 molecules. Five notable compounds with IDs 24334243, 24342416, 17516746, 17407455, and...
Staphylococcus aureus (S. aureus) has evolved as one of the most significant bacteria causing food poisoning outbreaks worldwide. This study was carried out to investigate prevalence, antibiotic sensitivity, virulence, and enterotoxin production S. in raw milk cow from small-scale units house-raised animals Damietta governorate, Egypt.The samples were examined bacteriologically, antimicrobial sensitivity testing out. Moreover, isolates characterized by molecular detection resistance,...
In a view of increasing the role Aeromonas spp. in fish and human diseases, present wok was conducted to assay presence six virulence genes using PCR species isolated from fishes water Manzala Lake.A total 200 samples comprising 100 Oreochromis niloticus fish, Mugil cephalus 50 were collected El Gamil region Lake Manzala.A number 258 isolates belonging spp.were recovered lake they biochemically identified into 4 biotypes.PCR 15 representatives, confirmed spp.isolates showed that genetically...
A total of 70 Aeromonas isolates isolated from 100 Oreochromis niloticus, Mugil cephalus and 50 water samples El Gamil region in Manzala Lake were investigated for antibiotic susceptibility test to 14 different antimicrobial agents using disc agar diffusion method.All strains showed (100%) sensitivity norfloxacin high cefotaxime (91.4%), gentamycin (90%), nalidixic acid (80%), amikacin (78.6%) chloramphenicol (74.3%).On the other hand, all tested resistant ampicillin, erythromycin penicillin...
This study was done on 200 Nile tilapia (Oreochromis niloticus) of different ages were collected from farms Central Laboratory for Aquaculture Research (Abbassa, Abou Hammad, Sharkia, Egypt) during period late November, 2014 to march, 2016. The fish samples clinically diseased showed, respiratory manifestation; ulcer and hemorrhages body surface, cotton wool like tufts skin, fins in eye caused opacity detached scale. Internally the affected showed enlarged pale or yellow, many cases...
A total number of 125 diseased rabbits collected from different farms in Port said Governorate during the period January 2016 till March 2017 showing clinical signs colisepticaemia ,with average age 1-2 month were examined, 625 samples liver, Intestine ,spleen, kidney and Heart blood (30.4%,29%,8.7%,17.4% and14.5%) positive for E.coli respectively. These isolates further characterized by polymerase chain reaction. was isolated 525 625samples (84%). The strain found to belong O stereotypes...
Total number of 200 apparently healthy fish, Mugil cephalus and Oreochromis niloticus100 each fish were collected randomly from different markets in Port-Said city to isolate identify Pseudomonas spp.from it the antibiotic sensitivity pattern isolated strains.Pseudomonas was found (66%) (80%) niloticus.Four hundred thirty six isolates identified as spp.and classified into P. fluorescens (29.13%),P. aeruginosa (27.06%),P. putida (11.01%),P.cepacia (10.55%),P. stutzeri (8.49%), anguilliseptica...
INTRODUCTION: Urinary tract infections (UTIs) is considered one of the most prevalent that may lead to many renal complications or dysfunctions. They are responsible for almost 10% all in Saudi Arabia, which makes them second common cause emergency department admission. Bacteria pathogens associated with UTIs. Nevertheless, UTIs caused by fungi have also been reported. Among fungal infections, Candida spp. has identified as primary UTI-related mycoses. 
 OBJECTIVE: To assess prevalence...
A total number of 350 random samples raw milk, pasteurized dairy products, tap water and udder wash were collected from markets in port-Said Province examined for prevalence Aeromonas species using starch ampicillin medium, The incidence isolated kariesh cheese, dmietta refrigerated white 58%, 26%, 70%, 48%, 40%, 16% 68%, respectively.The mesophilic isolates was 202 which 104 (51.5%) A. hydrophila, 60 (29.7%) caviae, 25 (12.4%)isolates sobria 11 (5.4%) schubertii.The highest (42) recovered...
The study was undertaken to detect the occurrence of E.coli in raw milk samples collected from different sources and around El- Arish city, North Sinai Governorate. A total 450 were animals farms, houses market (supermarkets, dairy shops, groceries vendors) situated El-Arish city surrounding areas. Various media used for cultivation organism; biochemical identification carried out by applying certain tests E.coli, as well serological using specific antisera. Congo red test vitro...
A total of 250 random samples meat and products 50 each meat, luncheon, burger, minced sausage were collected from different butchers in Port-Said Governorate.All subjected to bacteriological examination for isolation identification Staph.aureus.The results revealed that the Staph.aureus was isolated a percentage 55 (22%).The prevalence isolates examined 14%, 20%, 30% ,34% 12%, respectively.Coagulase positive (CPS) 33(60%) samples.CPS could be detected with 57.15%, 600%, 60%, 58.82% 66.66%,...
Eight hundred of meat products collected randomly from Port-Said markets for E. coli isolation.E. total prevalence was (43%).E. presence in minced (19.77%) followed by raw as (17.44%), sausage (17.15%), burger (16.57%), pastirma (15.70%), luncheon (6.10%), salami (4.07%), and frankfurter (3.20%).PCR showed that serotypes were positive (phoA) (tsh) while negative (stx1) ( Vt2e).E. O26, O125, O157 carried (eaeA).E. the only serotype (hly) (stx2).
Two hundred freshly slaughtered of balady chicken samples were collected aseptically from markets in Port-Said city during the period September 2016 to 2018.Nine Salmonella isolates (4.5%) isolated and therefore serologically identified.The Salmonellae Typhimurium 2/9 (22.2%) Muenster which was most frequent identified.Other serotypes as Enteritidis 1/9 (11.1%),Salmonella Blegdam Anatum Lamberhurst (11.1%) Ayinde also antibiogram revealed highly resistant Erythromycin Oxytetracycline by a...
Abstract: This study was conducted to investigate the prevalence, antimicrobial susceptibility, and molecular characterization of Aeromonas spp. from wild Nile tilapia Lake Manzala lake water. Swabs surface, gills, internal organs apparently healthy (n =100) water = 25) were collected examined bacteriologically for presence The isolates obtained tested their susceptibility 11 agents using disk diffusion method. antibiotic resistance genes (blaTEM, sul1, tetA(A), aadA1) virulence...
Pasteurella multocida has gained several mechanisms that developed antibiotic resistance due to the misuse and abuse of antibiotics. Deep screening bacterial molecular virulence factors will help find new threats for animal human diseases. Therefore, this research aims isolate identify P. from various affected Egyptian farms layers breeders study phenotypic characterization, antimicrobial sensitivity, PCR genotypic identification. Finally, pathogenicity isolated strains was evaluated in SPF...
This study discussed the prevalence of S. aureus in some hospitals Port-Said governorate and possibility impact isolates from foods animal origin on nosocomial infections by genetic relatedness between infected patients.The bacteriological examination 355 samples reveals 28 255 total taken kitchens with percentage 11% 48 100 swabs patients 48%, antibiotic sensitivity test most revealed multidrug resistant strains.Used random amplified polymorphic DNA (RAPD) patterns to determine among that...