A5021185544- Bone health and osteoporosis research
- Wnt/β-catenin signaling in development and cancer
- Connective tissue disorders research
- Tendon Structure and Treatment
- Hedgehog Signaling Pathway Studies
- Genetics and Physical Performance
- Hormonal and reproductive studies
- Cancer-related Molecular Pathways
- Bone Metabolism and Diseases
- Erythrocyte Function and Pathophysiology
- Skin Diseases and Diabetes
- Angiogenesis and VEGF in Cancer
- Genetic Mapping and Diversity in Plants and Animals
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Genomic variations and chromosomal abnormalities
- Exercise and Physiological Responses
- Mesenchymal stem cell research
- Muscle metabolism and nutrition
- Pain Mechanisms and Treatments
- Digestive system and related health
- Bone Tissue Engineering Materials
- Cerebral Palsy and Movement Disorders
- Myofascial pain diagnosis and treatment
- Glycosylation and Glycoproteins Research
Washington University in St. Louis
2017-2025
Michigan Medicine
2024-2025
University of Michigan
2025
Michigan United
2024
Bone formation via intramembranous and endochondral ossification is necessary for successful healing after a wide range of bone injuries. The pleiotropic cytokine, vascular endothelial growth factor A (VEGFA) has been shown, nonspecific pharmacologic inhibition, to be indispensable angiogenesis following fracture cortical defect repair. However, the importance VEGFA expression by different cell types during not well understood. We sought determine role from osteoblast subsets clinically...
SUMMARY Tendinopathy is a tendon disorder that caused by the failure to self-repair and has many pathological characteristics such as disorganized ECM decreased cell viability. We have identified possible target combat these changes, AMPK, an energy stress sensor was shown maintain intracellular homeostasis. Through bulk RNA-sequencing of healthy tendinopathic tendons from humans we novel finding downregulation AMPK signaling in samples which suggests plays role Our studies utilizing...
Purpose/aim Some youth seek puberty suppression to prolong decision-making prior starting hormone therapy help align their physical sex characteristics with gender identity. During peripubertal growth, connective tissues such as tendon rapidly adapt applied mechanical loads (e.g. exercise) yet if and how adaptation is influenced by gender-affirming during growth remains unknown. The goal of this study was understand pubertal followed testosterone influences the structural functional...
Mechanical loading is required for bone health and results in skeletal adaptation to optimize strength. Local nerve axons, particularly within the periosteum, may respond load-induced biomechanical biochemical cues. However, their role anabolic response remains controversial. We hypothesized that spatial alignment of periosteal nerves with sites formation would clarify this relationship. To achieve this, we developed RadialQuant, a custom tool histomorphometry. Tibiae control neurectomized...
Berardinelli-Seip congenital generalized lipodystrophy is associated with increased bone mass suggesting that fat tissue regulates the skeleton. Because there little mechanistic information regarding this issue, we generated "fat-free" (FF) mice completely lacking visible visceral, subcutaneous and brown fat. Due to robust osteoblastic activity, trabecular cortical volume markedly enhanced in these animals. FF mice, like patients, are diabetic but normalization of glucose tolerance...
Purpose: Studies have shown that marker-less motion detection systems, such as the first generation Kinect (Kinect 1), good reliability and potential for clinical application. of second 2) a large range accuracy relative to balance joint localization; however, few studies investigated validity 2 upper extremity motion. This investigation compared among 1, video capture (VMC) system movements.Design: One healthy, adult male performed six movements during two separate sessions. All were...
ABSTRACT Fracture healing is a complex process of many coordinated biological pathways. This system can go awry resulting in nonunion, which leads to significant patient morbidity. The Hedgehog (Hh) signaling pathway upregulated fracture healing. We hypothesized that the Hh be pharmacologically modulated positively affect Diaphyseal femur fractures were created elderly mice (18 months, C57BL/6 females), have blunted and delayed response compared younger mice, stabilized with intramedullary...
ABSTRACT Wnt signaling is critical to many aspects of skeletal regulation, but the importance ligands in bone anabolic response mechanical loading not well established. Recent transcriptome profiling studies by our laboratory and others show that potently induces genes encoding ligands, including Wnt1 Wnt7b. Based on these findings, we hypothesized stimulates adult formation inducing ligand expression. To test this hypothesis, inhibited secretion (5 months old) mice using a systemic (drug)...
Abstract Understanding the genetic basis of cortical bone traits can allow for discovery novel genes or biological pathways regulating health. Mice are most widely used mammalian model skeletal biology and quantification that cannot easily be evaluated in humans, such as osteocyte lacunar morphology. The goal our study was to investigate effect diversity on multi-scale 3 long bones skeletally-mature mice. We measured morphology, mechanical properties, material mineral composition mouse from...
Abstract The highly variable clinical outcomes noted after intrasynovial tendon repair have been associated with an early inflammatory response leading to the development of fibrovascular adhesions. Prior efforts broadly suppress this largely unsuccessful. Recent studies shown that selective inhibition IkappaB kinase beta (IKK‐β), upstream activator nuclear factor kappa‐light chain enhancer activated B cells (NF‐κB) signaling, mitigates and leads improved healing outcomes. In current study,...
Mechanical loading on the skeleton stimulates bone formation. Although exact mechanism underlying this process remains unknown, a growing body of evidence indicates that Wnt signaling pathway is necessary for skeletal response to loading. Recently, we showed Wnts produced by osteoblast lineage cells mediate osteo-anabolic tibial in adult mice. Here, report Wnt1 specifically plays crucial role mediating mechano-adaptive Independent loading, short-term loss Osx-lineage resulted decreased...
Knowledge of bone structure-function relationships in mice has been based on relatively small sample sets that limit generalizability. We sought to investigate long bones from a large population genetically diverse mice. Therefore, we analyzed previously published data the femur and radius male female F34 generation Large-by-Small advanced intercross line (LGXSM AI), which have over two-fold continuous spread body sizes (Silva et al. 2019 JBMR). Morphological traits, mechanical properties,...
Abstract Fracture healing is a complex process of many coordinated biological pathways. This system can go awry resulting in nonunion, which leads to significant patient morbidity. The Hedgehog (Hh) signaling pathway upregulated fracture healing. We hypothesized that the Hh be pharmacologically modulated positively affect Diaphyseal femur fractures were created elderly mice (18 months, C57BL/6 females), have blunted and delayed response compared younger mice, stabilized with intramedullary...
Mechanical loading is required for bone health and results in skeletal adaptation to optimize strength. Local nerve axons, particularly within the periosteum, may respond load-induced biomechanical biochemical cues. However, their role anabolic response remains controversial. We hypothesized that spatial alignment of periosteal nerves with sites formation would clarify this relationship. To achieve this, we developed RadialQuant, a custom tool histomorphometry. Tibiae control neurectomized...
Many transgender youth seek gender affirming care, such as puberty suppression, to prolong decision-making and align their physical sex characteristics with identity. During peripubertal growth, connective tissues tendon rapidly adapt applied mechanical loads (e.g., exercise) yet if how adaptation is influenced by hormone therapy during growth remains unknown. The goal of this study was understand the pubertal suppression influences structural functional properties Achilles using an...
Understanding the genetic basis of cortical bone traits can allow for discovery novel genes or biological pathways regulating health. Mice are most widely used mammalian model skeletal biology and quantification that can't easily be evaluated in humans, such as osteocyte lacunar morphology. The goal our study was to investigate effect diversity on multi-scale three long bones skeletally-mature mice. We measured morphology, mechanical properties, material mineral composition mouse from two...
Abstract Mechanical loading on the skeleton stimulates bone formation. Although exact mechanism underlying this process remains unknown, a growing body of evidence indicates that Wnt signaling pathway is necessary for skeletal response to loading. Recently, we showed Wnts produced by osteoblast lineage cells mediate osteo-anabolic tibial in adult mice. Here, report Wnt1 specifically plays crucial role mediating mechano-adaptive Independent loading, short-term loss Osx-lineage resulted...
Abstract Wnt signaling is critical to many aspects of skeletal regulation, but the importance ligands in adult bone homeostasis and anabolic response mechanical loading not well documented. We inhibited ligand secretion (5-mo) mice using a systemic (drug) bone-targeted (genetic) approach, subjected them axial tibial induce lamellar formation. Mice treated with porcupine inhibitor WNT974 exhibited decrease formation non-loaded limbs as 54% decline periosteal loading. Similarly, within 1-2...