A5101540937- Mesenchymal stem cell research
- Extracellular vesicles in disease
- 3D Printing in Biomedical Research
- Molecular Sensors and Ion Detection
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Sulfur Compounds in Biology
- Wound Healing and Treatments
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
- Collagen: Extraction and Characterization
- Toxic Organic Pollutants Impact
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Nitric Oxide and Endothelin Effects
- Tissue Engineering and Regenerative Medicine
- Nanofabrication and Lithography Techniques
- Effects and risks of endocrine disrupting chemicals
- CRISPR and Genetic Engineering
- Polymer Surface Interaction Studies
- Conducting polymers and applications
- Axon Guidance and Neuronal Signaling
- dental development and anomalies
- Environmental Science and Water Management
- Immune cells in cancer
Severance Hospital
2014-2024
Yonsei University
2011-2021
Korea University
2011-2021
Seoul Institute
2021
Government of the Republic of Korea
2021
Ansan University
2018
Mesenchymal stem cells (MSCs) are clinically useful due to their capacity for self-renewal, immunomodulatory properties and tissue regenerative potential. These can be isolated from various tissues exhibit different potential clinical applications according origin, thus comparative studies on MSCs essential. In this study, we investigated the immunophenotype, proliferative potential, multilineage differentiation of derived sources, namely bone marrow, adipose tissue, placenta umbilical cord...
Accumulating evidence has shown that the paracrine factors derived from mesenchymal stem cells (MSCs) are capable of regulating immune system via interaction with various cells. In this study, adipose-derived MSCs (AdMSCs) and human peripheral blood monocytes (PBMCs) were isolated cultured to examine effects MSC-induced macrophages (iMΦ) on inflammation modulation. Indirect coculture increased expression arginase-1 mannose receptor (CD206), markers activated M2 macrophages, in PBMCs...
Stem cell-derived exosomes are efficient and safe therapeutic tools for transferring endogenous biological cargo or functional biomolecules regenerative medicine. The regulation of inflammation angiogenesis plays a pivotal role in wound healing tissue regeneration. purpose this study was to investigate the anti-inflammatory pro-angiogenic roles human adipose mesenchymal stem exosomes, focusing on underlying mechanisms. Exosomes inhibited LPS-induced by activating ROCK1 PTEN expression....
Mesenchymal stem cell (MSC)-derived exosomes have emerged as an attractive cell-free tool in tissue engineering and regenerative medicine. The current study aimed to examine the anti-inflammatory, pro-angiogenic, wound-repair effects of both selenium-stimulated exosomes, check whether latter had superior wound healing capacity over others. cellular molecular network a paracrine signal, was extensively studied by performing miRNA arrays explore key mediators healing. Selenium is known play...
Viable mesenchymal stem cells (MSCs) were efficiently and selectively harvested by near-infrared (NIR) light using the photothermal effect of a conductive polymer nano thin film. The poly(3,4-ethylenedioxy thiophene) (PEDOT)-coated cell culture surfaces prepared via simple fast solution-casting polymerization (SCP) technique. absorption PEDOT films in NIR region was effectively triggered harvesting upon exposure to an source. By controlling film through electrochemical doping or growing with...
A bioreductant-resistant 'turn-on' chemodosimetric fluorescent probe Mito-1 has been developed for the detection of mitochondrial HNO in live cells. enables as low ∼18 nM. It capability to detect both exogenous and endogenous formations cellular milieus by providing fluorescence images. Its two-photon imaging ability fosters its use a noninvasive tool nitroxyl.
Introduction Sleep fragmentation (SF) is a hallmark of sleep disorders and has been associated with systemic health issues, but its specific impact on skin remains unclear. This study aimed to investigate whether SF impairs barrier function identify the biological pathways involved in SF-induced damage. Methods Twenty-four 6-week-old male BALB/c mice were divided into home cage control (HC) groups. was induced using commercially available chamber. Skin assessed by measuring transepidermal...
Near infrared (NIR) photothermal pattern on conductive polymer film enables unique approaches to harvest large‐area cell sheets with various patterns without the use of patterned culture dish. The NIR is generated from a optical lens (POL), which creates dynamic near IR light and corresponding (PTP) film. POL prepared transparent polydimethylsiloxane designed generate patterns. PTPs allow noninvasive cultured cells as an intact living sheet high harvesting efficiency (η > 100). Various...
The positive effects of mesenchymal stem cells (MSCs) are primarily activated through molecular secretions known as paracrine activity, which regulates the function various cell types including immune cells. Accumulating evidence shows that exosomes soluble factors released from MSCs potential alternative agents for cell-based therapy, although exact underlying mechanism has not been elucidated. purpose this study was to evaluate produced by adipose-derived and examine changes in...
Abstract The local heating of poly(3,4‐ethylenedioxythiophene) (PEDOT) by a photothermal effect directed near‐infrared (NIR) light induces unfolding absorbed collagen triple helices, yielding soluble single‐helical structures. This dissociation collagens allowed the harvesting living idiomorphic cell sheet, achieved upon irradiation with NIR ( λ =808 nm). PEDOT layer was patterned and cells were successfully cultured on substrate. Cell sheets various shapes mirroring pattern could be...
Mesenchymal stem cells (MSCs) differentiate into multiple lineages and are a promising source of for clinical use. Previously, we found that the gene distal‑less homeobox 5 (DLX5) is specifically expressed in MSCs with osteogenic potential. Understanding mechanism osteogenesis necessary successful bone regeneration using MSCs. The aim this study was to examine function DLX5 during (bone development). We analyzed possible association between expression osteogenesis-, chondrogenesis-...
Mesenchymal stem cells (MSCs) possess great therapeutic potential. Efficient in vitro expansion of MSCs is however necessary for their clinical application. The extracellular matrix (ECM) provides structural and biochemical support to the surrounding cells, it has been used as a coating substrate cell culture. In this study, we have aimed improve functionality stemness during culture using poly-L-lysine (PLL). Functionality was analysed by cycle analysis, differentiation assay,...
A cancer cell specific fluorescent probe is reported for sensing hydrogen sulfide.
Using a highly fluorescent, water-soluble polymer derived from triazine-bridged copolymer (DTMSPV), we explored the tunable fluorescence properties of DTMSPV by solvent polarity to function as sensory probe for protein sensing. The green-blue was significantly enhanced in presence bovine serum albumin through hydrophobic interactions. Meanwhile, complete quenching occurred hemoglobin iron complexation with polyelectrolyte. In addition, DTMSPVs were fluorescent and permeated into living...
Abstract Human adipose-derived stem cells (hADSCs) formed robust cell sheets by engineering the with soluble adhesive molecules (CAMs), which enabled unique approaches to harvest large area hADSC sheets. As a CAM, fibronectin (FN) (100 pg/ml) enhanced proliferation rate and control both cell-to-cell cell-to-substrate interactions. Through this of FN, transferrable sheet was obtained as free-stranding (122.6 mm 2 ) photothermal method. During harvesting method, collagen layer in-between...
Accumulating evidence has revealed that both high sensitivity C-reactive protein (hsCRP) and homocysteine (HCY) are associated with increased risk of metabolic syndrome (MetS) cardiovascular disease. However, it is unclear whether the coexistence these conditions accelerates (MetS). We hypothesized combination levels could exacerbate development MetS in a large prospective cohort study. selected data from 3,170 individuals (1,614 men 1,556 women) who participated Korean Genome Epidemiology...
The toxic effects of particulate matter have been linked to polycyclic aromatic hydrocarbons (PAHs) such as benzopyrene. PAHs are potent inducers the aryl hydrocarbon receptor (AhR), which is an expressed nuclear that senses environmental stimuli and modulates gene expression. Even though several studies shown benzopyrene (BP) chemical pollutants significantly impaired stem cell activity, exact molecular mechanisms were not clearly elucidated. In present study, we aimed investigate BP on...
Mesenchymal stem cells (MSCs) are an attractive resource for refractory patients because of their anti-inflammatory/immunomodulatory capability and multi-lineage differentiation potential. The transplantation MSCs has led to positive results in preclinical clinical application various diseases, including autoimmune disease, cardiovascular cancer, liver cirrhosis, ischemic stroke. On the other hand, studies have shown that paracrine factors, not direct cell replacement damaged or tissue, main...
Various micropatterned surfaces capable of guiding the selective adhesion biomolecules such as proteins and cells are great interests in biosensor, diagnostics, drug screening, tissue engineering. In this study, we described a simple photo-patterning method to prepare micro-patterned films for stem cell patterning using anthracene containing polymers (PMAn). This micro patterned polymer film was prepared by facile photo-reaction units backbone structure.The UV irradiation PMAn through...