A5010169945- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Reproductive Biology and Fertility
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- RNA modifications and cancer
- Genetic Syndromes and Imprinting
- Renal and related cancers
- Prenatal Screening and Diagnostics
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- RNA Research and Splicing
- Xenotransplantation and immune response
- Acute Myeloid Leukemia Research
- Muscle Physiology and Disorders
- Genetics, Aging, and Longevity in Model Organisms
- Molecular Biology Techniques and Applications
- Genetic Neurodegenerative Diseases
- Tissue Engineering and Regenerative Medicine
- Digestive system and related health
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurogenesis and neuroplasticity mechanisms
- 3D Printing in Biomedical Research
Korea Research Institute of Bioscience and Biotechnology
2014-2024
Korea University of Science and Technology
2012-2022
Chungnam National University
2007
Massachusetts General Hospital
2004
Harvard University
2004
Korea Advanced Institute of Science and Technology
1995-2000
Cell Biotech (South Korea)
1998
Methylation of histone H3 at lysine 9 (H3-K9) mediates heterochromatin formation by forming a binding site for HP1 and also participates in silencing gene expression euchromatic sites. ESET, G9a, SUV39-h1, SUV39-h2, Eu-HMTase are methyltransferases that catalyze H3-K9 methylation mammalian cells. Previous studies demonstrate the SUV39-h proteins preferentially targeted to pericentric heterochromatin, mice lacking both Suv39-h genes show cytogenetic abnormalities an increased incidence...
Abortions of nuclear transfer (NT) embryos are mainly due to insufficient placentation. We hypothesized that the primary cause might be aberrant allocations two different cell lineages blastocyst stage embryos, inner mass (ICM) and trophectoderm (TE) cells. The potential for development NT blastocysts was similar in vitro fertilized (IVF) embryos. No difference total number detected between IVF blastocysts, but both types had fewer cells than did vivo-derived (P < 0.05). showed a higher...
We investigated whether a genomic demethylation process occurs in pig preimplantation embryos produced by either normal fertilization or somatic cell nuclear transfer. The methylation status of the centromeric satellite and <i>PRE-1</i> short interspersed element (SINE) sequences was characterized using bisulfite-sequencing technology. Typical processes were identified these repetitive cloned donor genome during cleavage, patterns which similar to ones detected fertilized counterparts. These...
A viable cloned animal indicates that epigenetic status of the differentiated cell nucleus is reprogrammed to an embryonic totipotent state. However, molecular events regarding reprogramming somatic chromatin are poorly understood. Here we provide new insight chromatins refractory histone acetylation during early development. low level acetylated H4-lysine 5 (AcH4K5) was sustained at pronuclear stage. Unlike in vitro fertilized (IVF) embryos, AcH4K5 remarkably reduced 8-cell stage bovine...
Abstract DNA methylation reprogramming (DMR) is believed to be a key process by which mammalian zygotes gain nuclear totipotency through erasing epigenetic modifications acquired during gametogenesis. Nonetheless, DMR patterns do not seem conserved among mammals. To identify uniform rules underlying DMRs, we explored DMRs of diverse zygotes. Of the studied, particular interest was bovine zygote; paternal first decreased and then rapidly restored almost maternal level even before two‐cell...
SETDB1 (SET domain bifurcated-1) is a histone H3-lysine 9 (H3K9)-specific methyltransferase that mediates heterochromatin formation and repression of target genes. Despite the assumed functional link between DNA methylation SETDB1-mediated H3K9 trimethylations, several studies have shown operates autonomously in region- cell-specific manner. This study analyzes SETDB1-null HAP1 cells through linked methylome transcriptome analysis, intending to explore genes controlled by SETDB1-involved methylation.
Germline-stem cells (GSCs) produce gametes and are thus true "immortal stem cells". In Drosophila ovaries, GSCs divide asymmetrically to daughter cystoblasts, the latter differentiate into germline cysts. Here we show that histone-lysine methyltransferase dSETDB1, located in pericentric heterochromatin, catalyzes H3-K9 trimethylation their immediate descendants. As cysts egg chambers, dSETDB1 function is gradually taken over by another H3-K9-specific methyltransferase, SU(VAR)3-9....
Setdb1/Eset is a histone H3 lysine 9 (H3K9)-specific methyltransferase that associates with various transcription factors to regulate gene expression via chromatin remodeling. Here, we report Setdb1 promyelocytic leukemia (Pml) protein from the early stage of mouse development and constitutive member (PML)-nuclear bodies (PML-NBs) have been linked many cellular processes such as apoptosis, DNA damage responses, transcriptional regulation. Arsenic treatment, which induces Pml degradation,...
With a graying population and increasing longevity, it is essential to identify life transition in later years discern heterogeneity among older people. Subclassifying the elderly inspect subdivisions for pathophysiological differences particularly important investigation of age-related illnesses. For this purpose, using 24- 28-month-old mice represent "young-old" "old-old", respectively, we compared their skeletal muscle transcriptomes found each distinct stage: early/gradual (E-aging)...
This study was designed to examine the developmental ability of porcine embryos after somatic cell nuclear transfer. Porcine fibroblasts were isolated from fetuses at Day 40 gestation. In vitro-matured oocytes enucleated and electrically fused with cells. The reconstructed eggs activated using electrical stimulus cultured in vitro for 6 days. Nuclear-transferred (NT) a field strength 120 V/mm (11.6 ± 1.6%) showed higher rate as compared 150-V/mm group (6.5 2.3%) (P < 0.05), but mean numbers...
Abstract Background Studies have provided important findings about the roles of Notch signaling in neural development. Unfortunately, however, most these studies investigated stem cells (NSCs) mice or other laboratory animals rather than humans, mainly owing to difficulties associated with obtaining human brain samples. It prompted us focus on neuroectodermal spheres (NESs) which are derived from embryonic cell (hESC) and densely inhabited by NSCs. We here role hESC-derived NESs. Results...
In mammals, RNA interference is primarily a post-transcriptional mechanism. Evidence has accumulated for additional role in transcriptional gene silencing (TGS) but the question good paradigm small interfering antigene (agRNA)-induced chromatin modification remains unanswered. Here, we show that SETDB1, histone H3-lysine 9 (H3K9)-specific methyltransferase, cooperates with Argonaute-2 (AGO2) and plays an essential agRNA-induced TGS. The androgen receptor (AR) was transcriptionally silenced...
Redox adaptation is an important concept that explains the mechanisms by which cancer cells survive under persistent endogenous oxidative stress and become resistant to certain anticancer agents. To investigate this concept, we determined expression levels of peroxiredoxins (Prxs), antioxidant enzymes in drug-resistant non-small cell lung carcinoma cells. Prx II was remarkably increased only A549/GR (gefitinib-resistant) compared with A549 cells, consistent methylation/demethylation. highly...
Immediately after fertilization, a chromatin remodeling process in the oocyte cytoplasm extracts protamine molecules from sperm-derived DNA and loads histones onto it. We examined how histone H3-lysine 9 methylation system is established on remodeled sperm mice. found that paternal pronucleus was not stained for dimethylated H3-K9 (H3-m2K9) during development, while maternal genome intensively. Such H3-m2K9 asymmetry between parental pronuclei independent of HP1β localization and, much like...
S etdb1 is a histone H 3‐lysine 9 ( 3 K 9)‐specific methyltransferase that interacts with various transcriptional regulators to induce local heterochromatin formation and participates as an indispensable component in building promyelocytic leukemia nuclear body PML ‐ NB ), which involved biological processes. We studied the effects of over‐expression. unexpectedly observed exogenously expressed GFP was retained cytoplasm, whereas endogenous showed punctate signal. Leptomycin B LMB )...
We recently demonstrated that satellite regions exhibit an aberrant DNA methylation in cloned bovine embryos. Here, we examined, using bisulfite‐sequencing technology, whether the inefficient demethylation of donor genomes could be rescued by presence oocytic nuclei. Both Aci I digestion and sequencing analyses showed sequence was demethylated more efficiently tetraploid blastocysts than diploid clones. When methyl‐CpG density (the number sites per string) scored, a significant decrease...
DNA methylation reprogramming (DMR) during preimplantation development erases differentiation-associated, unessential epigenetic information accumulated gametogenesis, and ultimately brings pluripotency to the resulting embryo. Two patterns of DMR sperm-derived pronucleus have been reported in mammals. In first, male is actively demethylated whereas second, state seems be maintained. The maintenance-type has seen only through immunocytochemical observations, waits proven by additional...