A5001422415- Animal Genetics and Reproduction
- Xenotransplantation and immune response
- CRISPR and Genetic Engineering
- Virus-based gene therapy research
- Reproductive Biology and Fertility
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Organ Transplantation Techniques and Outcomes
- Viral Infectious Diseases and Gene Expression in Insects
- Liver physiology and pathology
- Pancreatic function and diabetes
- Animal Nutrition and Physiology
- Sperm and Testicular Function
- Probiotics and Fermented Foods
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Renal and related cancers
- Cell death mechanisms and regulation
- Viral gastroenteritis research and epidemiology
- Pharmacological Effects of Natural Compounds
- Gut microbiota and health
- Autophagy in Disease and Therapy
- Transplantation: Methods and Outcomes
- Digestive system and related health
- Epigenetics and DNA Methylation
Rural Development Administration
2016-2025
National Institute of Animal Science
2016-2025
National Cancer Center
2025
Korea Research Institute of Bioscience and Biotechnology
1999-2020
Seoul National University
2015
University of Nebraska Medical Center
2004-2012
Chonnam National University
2011
Kangwon National University
2011
The proximal domains of mitochondria and the endoplasmic reticulum (ER) are linked by tethering factors on each membrane, allowing efficient transport substances, including lipids calcium, between them. However, little is known about regulation function mitochondria-ER contacts (MERCs) dynamics under mitochondrial damage. In this study, we apply NanoBiT technology to develop MERBiT system, which enables measurement reversible MERCs formation in living cells. Analysis using system suggests...
Abortions of nuclear transfer (NT) embryos are mainly due to insufficient placentation. We hypothesized that the primary cause might be aberrant allocations two different cell lineages blastocyst stage embryos, inner mass (ICM) and trophectoderm (TE) cells. The potential for development NT blastocysts was similar in vitro fertilized (IVF) embryos. No difference total number detected between IVF blastocysts, but both types had fewer cells than did vivo-derived (P < 0.05). showed a higher...
The generation and application of porcine induced pluripotent stem cells (iPSCs) may enable the testing for safety efficacy therapy in field human regenerative medicine. Here, iPSCs from Massachusetts General Hospital miniature pig (MGH minipig) established organ transplantation studies is reported. Fibroblasts were isolated skin ear a 10-day-old MGH minipig transduced with cocktail six factors: POU5F1, NANOG, SOX2, C-MYC, KLF4, LIN28. Two distinct types generated that positive alkaline...
Reactive oxygen species (ROS) are potent regulators of stem cell behaviour; however, their physiological significance as regards MMP-mediated regulation the motility human umbilical cord blood-derived mesenchymal cells (UCB-MSCs) has not been characterized. In present study, we investigated role hydrogen peroxide (H2O2 ) and associated signalling pathways in promoting UCB-MSCs motility.The regulatory effects H2O2 on activation PKC, MAPKs, NF-κB β-catenin were determined. The expressions MMP...
The anti-inflammatory effects of oridonin (Ordn) have been well established in previous studies. However, the apoptotic Ordn on oral cancer cells not yet evaluated, at least to best our knowledge. aim this study was examine activity squamous cell carcinoma and eluciudate underlying mechanisms. For purpose, we employed experimental techniques, such as MTT assay, DAPI staining, soft agar flow cytometry western blot analysis. Our results revealed that suppressed proliferation colony formation,...
Kahweol, a diterpene molecule, has antiproliferative effects on several types of human cancer cells, but whether it apoptotic effect in non-small cell lung (NSCLC) is not known. To explore this possibility, we incubated cells from two NSCLC lines, NCI-H358 and NCI‑H1299, with different concentrations kahweol used the MTS assay, DAPI staining, propidium iodide Annexin V immunocytochemical test, western blot analysis to characterize molecule signaling pathway underlying its effects. The...
One of the reasons to causing blood coagulation in tissue xenografted organs was known incompatibility and anti-coagulation regulatory system between TG pigs primates. Thus, overexpression human CD73 (hCD73) pig endothelial cells is considered as a method reduce coagulopathy after pig-to-non-human-primate xenotransplantation. This study performed produce breed transgenic expressing hCD73 for studies immune rejection responses could provide successful application The were constructed an...
Bovine viral diarrhea (BVD) is a single-stranded, positive-sense ribonucleic acid (RNA) virus belonging to the genus Pestivirus of Flaviviridae family. BVD frequently causes economic losses farmers. Among bovine (BVDV) strains, BVDV-1b predominant and widespread in Hanwoo calves. Reverse-transcription polymerase chain reaction (RT-PCR) an essential method for diagnosing has become gold standard diagnosis Republic Korea. However, this diagnostic time-consuming requires expensive equipment....
Change of DNA methylation during preimplantation development is very dynamic, which brings this term to the most attractive experimental target for measuring capability cloned embryo reprogram its somatic genome. However, one weak point that stage carries little information on genomic sequences showing a site-specific re-methylation after global demethylation; these sequences, if any, may serve as an advanced subject test how exactly reprogramming/programming process recapitulated in early...
The Tumor Susceptibility Gene 101 (Tsg101) encodes a multi-domain protein that mediates variety of molecular and biological processes including the trafficking lysosomal degradation cell surface receptors. Conventional conditional knockout models have demonstrated an essential requirement this gene for cycle progression viability, but consequences complete ablation Tsg101 on intracellular not been examined to date. In study, we employed mouse embryonic fibroblasts carry two alleles...
The use of chlorella as an immune stimulant to enhance nonspecific host defense mechanisms or antimicrobial inhibit bacterial growth has been reported. Thus, the aim present study was clarify effect recombinant supplementation on performance, meat quality, and blood profile, excreta microflora, nutrient digestibility in broilers. A total 375 one-day-old ROSS 308 broilers (male female) were allotted 5 dietary treatments using cages with 15 chicks per cage. Treatments were: 1) NC, basal diet...
Soon after RNA-guided Cas9 (CRISPR-associated protein 9) endonuclease opened a new era of targeted genome editing, the CRISPR/Cas9 platform began to be extensively used modify genes in various types cells and organisms. However, successful CRISPR/Cas9-mediated insertion/deletion (indel) mutation remains demonstrated avian cell lines. The objective this study was design poultry-specific system efficiently introduce deletion chromosomes quail muscle clone 7 (QM7) line using customized CRISPR...
Esculetin, a coumarin derivative, is phenolic compound isolated from Artemisia capillaris, Citrus limonia, and Euphorbia lathyris.Although it has been reported to have anti-inflammatory, anti-oxidant, anti-proliferative activities in several human cancers, its activity against non-small-cell lung carcinoma (NSCLC) the molecular mechanisms involved not adequately elucidated.In this study, we used two NSCLC cell lines (NCI-H358 NCI-H1299) investigate apoptotic effect of esculetin.Our data...
Oxidative stress has been suggested to negatively affect oocyte and embryo quality developmental competence, resulting in failure reach full term. In this study, we investigated the effect of N-acetyl-L-cysteine (NAC), a cell-permeating antioxidant, on competence oocytes embryos upon supplementation (0.1–10 mM) maturation culture medium vitro using slaughterhouse-derived embryos. The results show that treating with 1.0 mM NAC for 8 h during attenuated intracellular reactive oxygen species...
The pig genome contains the gamma 1 family of porcine endogenous retroviruses (PERVs), which are a major obstacle to development successful xenotransplantation from human. Long terminal repeats (LTRs) found in PERVs known be essential elements for control transcriptional activity single virus by different transcription factors (TFs). To identify transcribed PERV LTR elements, RT-PCR and DNA sequencing analyses were performed. Twenty-nine actively identified kidney tissues NIH-Miniature pig....
Abstract Xenotransplantation of pig organs into primates leads to hyperacute rejection (HAR). Functional ablation the α1,3-galactosyltransferase (GalT) gene, which abrogates expression Galα1-3Galβ1-4GlcNAc-R (Gal) antigen, inhibits HAR. However, antigens other than Gal may induce immunological by their cognate antibody responses. Ultimately, overexpression complement regulatory proteins reduces acute humoral non-Gal antibodies when GalT is ablated. In this study, we developed a vector-based...