A5078026079- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Xenotransplantation and immune response
- Genetic and phenotypic traits in livestock
- Tissue Engineering and Regenerative Medicine
- Reproductive Physiology in Livestock
- Animal Behavior and Welfare Studies
- Diverse Topics in Contemporary Research
- Renal and related cancers
- Genetic Mapping and Diversity in Plants and Animals
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- Nutrition, Health and Food Behavior
- Advanced Glycation End Products research
- Polyamine Metabolism and Applications
- Sperm and Testicular Function
- Reproductive System and Pregnancy
- Pancreatic function and diabetes
- Parasitic Infections and Diagnostics
- Alkaline Phosphatase Research Studies
- Agriculture, Soil, Plant Science
- Ultrasound and Hyperthermia Applications
Jeonbuk National University
2015-2023
National Institute of Animal Science
2013-2017
Rural Development Administration
2013-2016
Recent progress in genetic manipulation of pigs designated for xenotransplantation ha6s shown considerable promise on xenograft survival primates. However, modification multiple genes donor by knock-out and knock-in technologies, aiming to enhance immunological tolerance against transplanted organs the recipients, has not been evaluated health issues pigs. We produced transgenic Massachusetts General Hospital piglets knocking-out α-1,3-galactosyltransferase (GT) gene simultaneously...
The generation and application of porcine induced pluripotent stem cells (iPSCs) may enable the testing for safety efficacy therapy in field human regenerative medicine. Here, iPSCs from Massachusetts General Hospital miniature pig (MGH minipig) established organ transplantation studies is reported. Fibroblasts were isolated skin ear a 10-day-old MGH minipig transduced with cocktail six factors: POU5F1, NANOG, SOX2, C-MYC, KLF4, LIN28. Two distinct types generated that positive alkaline...
Oct4 is a crucial germ line‐specific transcription factor expressed in different pluripotent cells and downregulated the process of differentiation. There are two conserved enhancers, called distal enhancer (DE) proximal (PE), 5′ upstream regulatory sequences (URSs) mouse gene, which were demonstrated to control expression independently embryonic stem (ESCs) epiblast (EpiSCs). We analyzed URSs pig identified similar enhancers that highly consistent with DE PE. A dual‐fluorescence reporter...
Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect cell cycle inhibitors on synchronization piPSCs. piPSCs were generated using combination six human transcriptional factors under culture condition. To examine synchronization, cultured a matrigel coated plate with media and they treated staurosporine (STA, 20 nM), daidzein (DAI, 100 μM), roscovitine (ROSC, 10...
Pigs share many physiological, anatomical and genomic similarities with humans, which make them suitable models for biomedical researches. Understanding the genetic status of Yucatan miniature pigs (YMPs) their association human diseases will help to assess potential as model animals. This study was performed identify non-synonymous single nucleotide polymorphisms (nsSNPs) in selective sweep regions genome YMPs present nsSNP distributions that are potentially associated disease occurrence...
Pretreatment of somatic cells with undifferentiated cell extracts, such as embryonic stem and mammalian oocytes, is an attractive alternative method for reprogramming control. The properties induced pluripotent (iPSCs) are similar to those cells; however, no studies have reported nuclear using iPSC extracts. Therefore, this study aimed evaluate the effects porcine extracts treatment on ear fibroblasts early development cloned embryos produced from skin pretreated ChariotTM reagent system was...
The Korean native pig (KNP) have been considered as animal models for biotechnology research because of their relatively small body size and presumably highly inbred status due to the closed breeding program. However, little is reported about use KNP researches. This study was performed establish somatic cell nuclear transfer (SCNT) protocol production swine leukocyte antigens (SLA) homotype-defined SCNT KNP. ear fibroblast cells originated from were cultured used donor cell. After thawing,...
Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit eukaryotic ribosome. This has an important role in synthesis and occurrence hereditary diseases. Pig common laboratory model, however, to best our knowledge, its RPL21 gene not been cloned date. In this study, we identified full-length sequence pig for first time. addition, examined expression pattern function by using overexpression or knockdown approaches. As result, obtained 604 bp segment that contains 483 open...
SUMMARY The mammary gland serves as a valuable bioreactor system for the production of recombinant proteins in lactating animals. Pharmaceutical‐grade protein can be harvested from milk transgenic animals that carry interest under control promoter regions genes encoding proteins. Whey acidic ( WAP ), example, is predominantly expressed and regulated by hormones during pregnancy. We cloned 5′‐flanking region porcine gene (p ) to confirm sequence elements its are required gene‐expression...
Cloned pigs produced by somatic cell nuclear transfer (SCNT) are important as a potential alternative source of organs. Although SCNT has created new possibilities for targeted gene modification, the successful cloning is rare. Here, we successfully conducted serial three generations. We determined that piglet genome was inherited from donor nuclei using microsatellite analysis each generation. The methylation differentially methylated regions (DMRs) in H19 gradually reduced over generations...
Pigs have been used as a good research model for xenotransplantation. Several groups generated porcine-induced pluripotent stem cells (piPSCs) from differentiated somatic cells. Transgenic pigs with the alpha1,3-galactosyltransferase gene-knockout (GalT-KO) could successfully govern hyper acute rejection of organ transplants into primates. Thus, GalT-KO piPSCs be powerful cell resource agricultural and biomedical applications. This study was performed to generate iPSCs characterize their...
In-Sul Hwang, Dae-Jin Kwon, Tae-Uk Kwak, Joo-Young Lee, Nam-Woong Hyung, Hyeon Yang, Keon Bong Oh, Sun-A Ock, Eung-Woo Park, Gi-Sun Im, and Seongsoo Hwang. Journal of Embryo Transfer 2016;31:117-21. https://doi.org/10.12750/JET.2016.31.2.117
Jae-Hoon Yeo, In-Sul Hwang, Jae Kyung Park, Dae-Jin Kwon, Seoki Im, Eung-Woo Jeong-Woong Lee, Choon-Keun Park and Seongsoo Hwang. Journal of Embryo Transfer 2014;29:339-44. https://doi.org/10.12750/JET.2014.29.4.339
Sun A Ock , Keon Bong Oh, Seongsoo Hwang, Jungkyu Lee, Youngim Kim, Sun-Woung Moon, Dae-Jin Kwon, Ik Jin Yun and Eungwoo Park. Journal of Embryo Transfer 2014;29:333-7. https://doi.org/10.12750/JET.2014.29.4.333
This study aimed at investigating whether a porcine follicular fluid (pFF) supplementation positively affects the characteristics of donor cells and developmental competence cloned embryos. Ear fibroblast (donor cell) from an Massachusetts General Hospital miniature pig were cultured in different culture methods: (1) Dulbecco's modified Eagle's medium (DMEM)+10% FBS (Control); (2) DMEM+0.5% (SS); (3) DMEM+10% FBS+10% pFF for 72 h. In each conditioned medium, concentrations 4 amino acids...