A5017958553- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Xenotransplantation and immune response
- Genetic Syndromes and Imprinting
- Prenatal Screening and Diagnostics
- Epigenetics and DNA Methylation
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Sperm and Testicular Function
- Ovarian function and disorders
- Metabolism and Genetic Disorders
- Pregnancy and preeclampsia studies
- Cancer-related molecular mechanisms research
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Mitochondrial Function and Pathology
- Autonomous Vehicle Technology and Safety
- Muscle metabolism and nutrition
- Video Surveillance and Tracking Methods
- Mesenchymal stem cell research
National Institute of Animal Science
2016-2025
Rural Development Administration
2017-2025
Electronics and Telecommunications Research Institute
2016
Konkuk University
2010-2013
Genomic imprinting is an epigenetic process that results in parent-of-origin effects on mammalian development and growth. Research genomic domesticated animals has lagged due to a primary focus orthologs of mouse human imprinted genes. This emphasis limited the discovery genes specific livestock. To identify pigs, we generated parthenogenetic porcine embryos alongside biparental normal embryos, then performed whole-genome bisulfite sequencing RNA these samples. In our analyses, discovered...
Genomic reprogramming factors in the cytoplasm of germinal vesicle (GV) stage oocytes have been shown to improve efficiency producing cloned mouse offspring through exposure nuclei a GV cytoplasmic extract prior somatic cell nuclear transfer (SCNT) enucleated oocytes. Here, we developed an pig (GVcyto-extract) investigate epigenetic events treated nuclei. This induced differentiation-associated changes fibroblasts, resulting cells that exhibit pluripotent stem cell-like characteristics and...
Embryo development is a complex and tightly controlled process. Nanoparticle injury can affect normal lead to malformation or miscarriage of the embryo. However, risk that these nanoparticles may pose reproduction not clear. In this study, chitosan (CSNP) near uniform size, in range 100 nm, were synthesized confirmed by particle size analyzer transmission electron microscopy. Morulae-stage embryo exposure CSNP during vitro culture caused blastocyst complications had either no cavity small...
Busulfan and cyclophosphamide (B/C)-treated mice exhibited a marked increase in apoptosis concomitant decrease the ovarian weight. Histological RT-PCR analysis indicate that period of germ cell depletion B/C-treated ovaries coincides with decreased expression genes Figla, Lhx8, Nobox, c-kit, Sox3. However, cells is mediated by autophagy-independent pathways involve Fas/FasL-, TNF-, and/or p53-signalings. Treatment B/C resulted cease reproductive function to produce their offspring during...
Alzheimer's disease (AD) is a progressive neurodegenerative associated with memory loss and cognitive impairments. An AD transgenic (Tg) pig model would be useful for preclinical testing of therapeutic agents. We generated an Tg by somatic cell nuclear transfer (SCNT) using multi-cistronic vector that harbored three AD-related genes total six well-characterized mutations: hAPP (K670N/M671L, I716V, V717I), hTau (P301L), hPS1 (M146V L286P). Four lines were established from Jeju black ear...
In this study, we examined whether Hanganutziu-Deicher (H-D) antigens are important as an immunogenic non-α1,3-galactose (Gal) epitope in pigs with a disrupted α1,3-galactosyltransferase gene. The targeting efficiency of the AO blood genotype was achieved (2.2%) pig fibroblast cells. A total 1800 somatic cell nuclear transfer (SCNT) embryos were transferred to 10 recipients. One recipient developed term and naturally delivered two piglets. activity lung, liver, spleen, testis heterozygote...
Bovine viral diarrhea (BVD) is a single-stranded, positive-sense ribonucleic acid (RNA) virus belonging to the genus Pestivirus of Flaviviridae family. BVD frequently causes economic losses farmers. Among bovine (BVDV) strains, BVDV-1b predominant and widespread in Hanwoo calves. Reverse-transcription polymerase chain reaction (RT-PCR) an essential method for diagnosing has become gold standard diagnosis Republic Korea. However, this diagnostic time-consuming requires expensive equipment....
We examined whether deficiency of the GGTA1 gene in pigs altered expression several glycosyltransferase genes. Real-time RT-PCR and activity showed that 2 sialyltransferases [α2,3-sialyltransferase (α2,3ST) α2,6-sialyltransferase (α2,6ST)] heterozygote GalT KO liver have higher levels activities compared to controls. Enzyme-linked lectin assays indicated there were also more sialic acid-containing glycoconjugate epitopes livers than The elevated level sialic-acid-containing was due low α-Gal...
One of the reasons to causing blood coagulation in tissue xenografted organs was known incompatibility and anti-coagulation regulatory system between TG pigs primates. Thus, overexpression human CD73 (hCD73) pig endothelial cells is considered as a method reduce coagulopathy after pig-to-non-human-primate xenotransplantation. This study performed produce breed transgenic expressing hCD73 for studies immune rejection responses could provide successful application The were constructed an...
Oxidative stress has been suggested to negatively affect oocyte and embryo quality developmental competence, resulting in failure reach full term. In this study, we investigated the effect of N-acetyl-L-cysteine (NAC), a cell-permeating antioxidant, on competence oocytes embryos upon supplementation (0.1–10 mM) maturation culture medium vitro using slaughterhouse-derived embryos. The results show that treating with 1.0 mM NAC for 8 h during attenuated intracellular reactive oxygen species...
Abstract Although it is known that the tetraploid embryo contributes only to placenta, question of why embryos differentiate into placenta remains unclear. To study effect electrofusion on development mouse oocytes, two‐cell were fused and cultured in vitro Chatot‐Ziomek‐Bavister medium. After electrofusion, two chromosome sets from blastomere individually duplicated before nuclear fusion. At 8–10 hr after each set was condensing membrane breaking down. Around 12–14 had combined together...
In mammals, genomic imprinting operates via gene silencing mechanisms. Although conservation of the mechanism at H19 / IGF2 locus has been generally described in pigs, tissue-specific transcript level, monoallelic-to-biallelic conversion, and spatio-temporal chromatin reorganization remain largely uninvestigated. Here, we delineate spatially regulated transcripts, age-dependent hepatic mono- to biallelic topologically associating domains porcine H19/IGF2 for better translation human animal...
The difference between early pregnancy and delivery rate is quite large in assisted reproduction techniques (ARTs), including animal cloning. However, it not clear why the implanted fetuses aborted after stage. In present study, we tried to evaluate developmental morphological characteristics of porcine parthenogenetically activated (PA) embryos or by electric stimulation during period. PA artificially inseminated (AI) were collected at day 26 35 embryo transfer, respectively. parameters...
Porcine heart xenotransplantation is a potential treatment for patients with end-stage failure. To understand molecular mechanisms of graft rejection after transplantation, we transplanted 31-day-old alpha-1,3-galactosyltransferase knockout (GTKO) porcine to five-year-old cynomolgus monkey. Histological and transcriptome analyses were conducted on xenografted cardiac tissue at (nine days transplantation). The recipient monkey’s blood parameters analyzed −7, −3, 1, 4, 7. Validation was by...
In this study, it was demonstrated that tetraploid-derived blastocyst embryos had very few Oct4-positive cells at the mid-blastocyst stage and inner cell mass biomarkers Oct4, Sox2 Klf4 expressed less than 10% of level observed in diploid blastocysts. contrast, trophectoderm-related gene transcripts showed an approximately 10 to 40% increase. Of 32,996 individual mouse genes evaluated by microarray, 50 were differentially between tetraploid or parthenote (P<0.05). these genes, 28 more highly...
In mammals, imprinted genes often exist in the form of clusters specific chromosome regions. However, pigs, genomic imprinting a relatively few and has been identified, within or adjacent to putative need be investigated including those at SGCE/PEG10 locus. The objective this study was to, using porcine parthenogenetic embryos, investigate status region spans between COL1A2 ASB4 9. Whole-genome bisulfite sequencing (WGBS) RNA (RNA-seq) were conducted with normal methylome transcriptome...
Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect cell cycle inhibitors on synchronization piPSCs. piPSCs were generated using combination six human transcriptional factors under culture condition. To examine synchronization, cultured a matrigel coated plate with media and they treated staurosporine (STA, 20 nM), daidzein (DAI, 100 μM), roscovitine (ROSC, 10...
Gene expression profiling of compromised umbilical cords (CUCs) derived from somatic cell nuclear transfer (scNT) clones was performed to determine why scNT-derived often exhibit malformed arteries. Umbilical cord samples were obtained 65 scNT piglets, and these, nine displayed a CUC. Microscopic analyses the with CUCs (scNT-CUCs) revealed complete occlusive thrombi that not detected in arteries normal (scNT-Ns). Moreover, whereas allantoic ducts scNT-Ns contained columnar epithelium,...
The primary differentially methylated regions (DMRs) which are maternally hypermethylated serve as imprinting control (ICRs) that drive monoallelic gene expression, and these ICRs have been investigated due to their implications in mammalian development. Although a subset of genes has identified imprinted, in-depth comparative approach needs be developed for identification species-specific imprinted genes. Here, we examined DNA methylation status allelic expression at the KBTBD6 locus across...
cd26 is ubiquitously distributed in the body, particularly endothelial and epithelial cells, with highest expression kidney, liver, small intestine. In humans, serves as a marker for embryo implantation phase. However, little known about role of porcine pre-implantation development. Here, we aimed to examine siRNA-induced downregulation cytoplasm MII oocytes, determine whether involved regulation embryonic The siRNA was micro-injected into which were then parthenogenetically activated...
There was an error published in Development139, 4330-4340.Nguyen Van Thuan incorrectly listed as author for correspondence. The correct authors correspondence are shown below:*Authors (bthuy@konkuk.ac.kr; jhkim541@konkuk.ac.kr).The apologise to Nguyen and readers this mistake.