A5001019600- Acute Myeloid Leukemia Research
- dental development and anomalies
- Immune Cell Function and Interaction
- Plant Disease Management Techniques
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
- Plant Physiology and Cultivation Studies
- T-cell and B-cell Immunology
- Wnt/β-catenin signaling in development and cancer
- Genomics and Chromatin Dynamics
- Plant Virus Research Studies
- Cancer-related gene regulation
- Cytokine Signaling Pathways and Interactions
- Epigenetics and DNA Methylation
- Oral and Maxillofacial Pathology
- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Agriculture, Soil, Plant Science
- Pluripotent Stem Cells Research
- Radiopharmaceutical Chemistry and Applications
- Bone and Dental Protein Studies
- Cleft Lip and Palate Research
- Gene expression and cancer classification
- RNA Research and Splicing
- Protein Degradation and Inhibitors
Kyungpook National University
2014-2024
Memorial Sloan Kettering Cancer Center
2015-2024
Rural Development Administration
2006-2023
National Institute of Agricultural Science and Technology
2022-2023
Chungnam National University
2016
Life University
2016
Seoul St. Mary's Hospital
2014
Seoul National University
2014
Catholic University of Korea
2014
Sunchon National University
2013
We have analyzed a systematic flaw in the current system of gene identification: oligo(dT) primer widely used for cDNA synthesis generates high frequency truncated cDNAs through internal poly(A) priming. Such may contribute to 12% expressed sequence tags dbEST database. By using synthetic transcript and real mRNA templates as models, we characterized patterns priming by primer. further demonstrated that can be effectively diminished replacing with set anchored primers reverse transcription....
The number of genes in the human genome is still a controversial issue. Whereas most are said to have been physically or computationally identified, many short cDNA sequences identified as tags by use serial analysis gene expression (SAGE) do not match these genes. By performing experimental verification more than 1,000 SAGE and analyzing 4,285,923 origin current database, we examined nature unmatched tags. Our study shows that truly novel originated from transcripts yet genome, including...
Isolation of low-abundance transcripts expressed in a genome remains serious challenge transcriptome studies. The sensitivity the methods used for analysis has direct impact on efficiency detection. We compared EST method and SAGE to determine which one is more sensitive what extent great detection transcripts. Using same detected by both as targeted sequences, we observed that 26 times than efficient detecting
The biological effect of radiofrequency (RF) fields remains controversial. We address this issue by examining whether RF can cause changes in gene expression. used the pulsed at a frequency 2.45 GHz that is commonly telecommunication to expose cultured human HL‐60 cells. serial analysis expression (SAGE) method measure on genome level. observed 221 genes altered their after 2‐h exposure. number affected increased 759 6‐h Functional classification reveals apoptosis‐related were among...
Using the serial analysis of gene expression technique, we surveyed transcriptomes three major tissues (panicles, leaves, and roots) a super-hybrid rice (Oryza sativa) strain, LYP9, in comparison to its parental cultivars, 93-11 (indica) PA64s (japonica). We acquired 465,679 tags from libraries, which were consolidated into 68,483 unique tags. Focusing our initial functional analyses on subset data that are supported by full-length cDNAs (genes) differentially expressed hybrid at significant...
We have analyzed the pattern of gene expression in human primary CD34 + stem/progenitor cells. identified 42,399 unique serial analysis (SAGE) tags among 106,021 SAGE collected from 2.5 × 10 6 cells purified bone marrow. Of these tags, 21,546 matched known expressed sequences, including 3,687 genes, and 20,854 were novel without a match. The that sequences tended to be at higher levels, whereas lower levels. By using generation longer for identification (GLGI) method, we correct 385 440...
In recent reports, we have shown that optimized pretargeted radioimmunotherapy (PRIT) based on molecularly engineered antibody conjugates and 177 Lu-DOTA chelate (DOTA-PRIT) can be used to cure mice bearing human solid tumor xenografts using antitumor antibodies minimally internalizing membrane antigens, GPA33 (colon) GD2 (neuroblastoma).However, many antigens are internalized after binding it is generally believed not suitable targets for PRIT.In this study, tested the hypothesis DOTA-PRIT...
To identify changes in the regulation of B cell receptor (BCR) signals during development human cells, we generated genome-wide gene expression profiles using serial analysis (SAGE) technique for CD34+ hematopoietic stem cells (HSCs), pre-B naive, germinal center (GC), and memory cells. Comparing these SAGE profiles, genes encoding positive regulators BCR signaling were expressed at consistently lower levels naive than all other subsets. Conversely, a large group inhibitory molecules, mostly...
Abstract Serial analysis of gene expression (SAGE) is a powerful technique for genome‐wide expression. However, two‐thirds SAGE tags cannot be used directly identification two reasons. First, many match several known expressed sequences, owing to the short length tag sequences. Second, do not any presumably because sequences corresponding these have been identified. These problems can solved by extension into 3′ complementary DNAs (cDNAs) use GLGI (generation longer cDNA fragments from...
The relationship between allergic inflammation and gut microbiota has been elucidated, the effect of probiotics on immune disorders studied as well. Identifying role in individual diseases responses selecting applying specific microorganisms based these findings can be an effective strategy for using probiotics. Herein, lactobacilli isolated from kimchi were investigated depth, focusing their regulatory effects mechanisms involved. Lactic acid bacteria (LAB) effectively diminished increased...
Abstract The NUP98 gene has been reported to be fused with at least 15 partner genes in leukemias 11p15 translocations. We report the results of screening cases cytogenetically documented rearrangements and subsequent identification involvement its genes. identified 49 samples from 46 hematology patients (including a few 11p14) abnormalities, using fluorescence situ hybridization (FISH), we found that was disrupted 7 cases. With use gene‐specific FISH probes, 6 cases, genes, which were PRRX1...
Increasing evidence suggests that low-abundant transcripts may play fundamental roles in biological processes. In an attempt to estimate the prevalence of eukaryotic genomes, we performed a transcriptome analysis Drosophila using SAGE technique. We collected 244,313 tags from expressed embryonic, larval, pupae, adult, and testicular tissue. From these tags, identified 40,823 unique tags. Our showed 55% are novel without matches currently known transcripts, most have low copy numbers. Further...
Identification of the specific cytogenetic abnormality is one critical steps for classification acute myeloblastic leukemia (AML) which influences selection appropriate therapy and provides information about disease prognosis. However at present, genetic complexity AML only partially understood. To obtain a comprehensive, unbiased, quantitative measure, we performed serial analysis gene expression (SAGE) on CD15 + myeloid progenitor cells from 22 patients who had four most common...
Juxtaposed with another zinc finger protein 1 (Jazf1) is a and known to affect both prostate cancer type 2 diabetes. Jazf1 inhibits testicular nuclear receptor 4 (TR4) activation through protein-protein interaction, which results in weight loss alleviates However, the role of still poorly understood. Hence, we investigated whether expression associated progression. We confirmed upregulation human tissue samples. In addition, using overexpressing cell lines, DU145 LNCaP, found promoted...
Ten-eleven translocation methylcytosine dioxygenase 1 (Teil) initiates DNA demethylation by converting 5-methylcytosine (5-mC) to 5-hydroxymethylcytosine (5-hmC) at CpG-rich regions of genes, which have key roles in adult neurogenesis and memory. In addition, the overexpression Tet1 with 5-hmC alteration patients psychosis has also been reported, for instance schizophrenia bipolar disorders. The mechanism underlying brain; however, is still elusive. present study, we found that...
In an attempt to characterize early B cell development including the commitment of progenitor cells lineage, we generated and compared genomewide gene expression profiles human hematopoietic stem (HSCs) pre-B (PBCs) by using serial analysis expression. From more than 100,000 tags collected from CD34 + HSCs CD10 CD19 PBCs, 42,399 unique transcripts were identified in but only 16,786 suggesting that 60% expressed silenced during or after lineage. On other hand, mRNAs receptor...