A5079175227- Monoclonal and Polyclonal Antibodies Research
- Radiopharmaceutical Chemistry and Applications
- Neuroblastoma Research and Treatments
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Lung Cancer Research Studies
- HER2/EGFR in Cancer Research
- Gastric Cancer Management and Outcomes
- Lung Cancer Diagnosis and Treatment
- Supramolecular Self-Assembly in Materials
- Diatoms and Algae Research
- Glycosylation and Glycoproteins Research
- Helicobacter pylori-related gastroenterology studies
- Cancer, Hypoxia, and Metabolism
- Colorectal Cancer Screening and Detection
- Pancreatic and Hepatic Oncology Research
- Medical Imaging and Pathology Studies
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Medical Imaging Techniques and Applications
- Luminescence and Fluorescent Materials
First Hospital of Jilin University
2014-2024
Jilin University
2014-2024
Memorial Sloan Kettering Cancer Center
2010-2024
Soochow University
2015-2024
First Affiliated Hospital of Soochow University
2016-2024
Kettering University
2010-2020
Creative Commons
2020
Unidad de Cirugía Artroscópica
2020
University of Oklahoma
2011-2018
Norman Regional Hospital
2015
Background and AimsArtificial intelligence (AI)–assisted colonoscopy improves polyp detection characterization in colonoscopy. However, data from large-scale multicenter randomized controlled trials (RCT) an asymptomatic population are lacking.MethodsThis RCT aimed to compare AI-assisted with conventional for adenoma population. Asymptomatic subjects 45–75 years of age undergoing colorectal cancer screening by direct or fecal immunochemical test were recruited 6 referral centers Hong Kong,...
Abstract B7-H3, a surface immunomodulatory glycoprotein, inhibits natural killer cells and T cells. The monoclonal antibody (mAb) 8H9 is specific for 4Ig-B7-H3, the long principal form of B7-H3. Early results from radioimmunotherapy using have shown promise in patients with metastatic solid tumors to central nervous system. Whereas B7-H3 transcript was ubiquitously expressed wide spectrum human as well normal tissues, protein preferentially only tumor tissues. By quantitative reverse...
This paper described the energy-transfer amplified singlet oxygen generation in semiconductor polymer dots (Pdots) for vitro and vivo photodynamic therapy. Hydrophobic photosensitizer tetraphenylporphyrin was facilely doped nanoparticles consisting of densely packed polymers. Optical characterizations indicated that fluorescence Pdots completely quenched by photosensitizer, yielding an energy transfer efficiency nearly 100% singlet-oxygen quantum yield ∼50%. We evaluated cellular uptake,...
Gastric cancer is a common malignancy with high mortality. Long noncoding RNA (lncRNA) zinc finger antisense (ZFAS)1 upregulated in gastric specimens compared the para-carcinoma tissues. The silencing of ZFAS1 inhibited growth, proliferation, cell cycle progress, migration, invasion and epithelial-mesenchymal transition (EMT), enhanced sensitivity to cis-platinum or paclitaxel SGC7901 cells, as evidenced by expression changes proliferating nuclear antigen, Cyclin D1, E, B1, E-cadherin,...
Helicobacter pylori ( H. ) is the predominant pathogen causing chronic gastric mucosal infections globally. During period from 2011 to 2022, global prevalence of infection was estimated at 43.1%, while in China, it slightly higher approximately 44.2%. Persistent colonization by can lead gastritis, peptic ulcers, and malignancies such as mucosa-associated lymphoid tissue (MALT) lymphomas adenocarcinomas. Despite eliciting robust immune responses host, thrives mucosa modulating host immunity,...
Candida albicans (C. albicans) infection causes high mortality rates within cancer patients. Due to the low sensitivity of current diagnosis systems, a new sensitive detection method is needed for its diagnosis. Toward this end, here we exploited capability genetically displaying two functional peptides, one responsible recognizing biomarker (antisecreted aspartyl proteinase 2 IgG antibody) in sera patients and another binding magnetic nanoparticles (MNPs), on single filamentous fd phage,...
Abstract Anti-disialoganglioside GD2 IgG antibodies have shown clinical efficacy in solid tumors that lack human leukocyte antigens (e.g., neuroblastoma) by relying on Fc-dependent cytotoxicity. However, there are pain side effects secondary to complement activation. T-cell retargeting bispecific (BsAb) also potential, but it is thus far only effective against liquid tumors. In this study, a fully humanized hu3F8-BsAb was developed, which the anti-CD3 huOKT3 single-chain Fv fragment (ScFv)...
T-cell-based therapies have emerged as one of the most clinically effective ways to target solid and non-solid tumors. HER2 is responsible for oncogenesis treatment resistance several human As a member HER family tyrosine kinase receptors, its over-activity confers unfavorable clinical outcome. Targeted directed at this receptor achieved responses, although development common. We explored novel HER2/CD3 bispecific antibody (HER2-BsAb) platform that while preserving anti-proliferative effects...
Bioinspired mineralization is an innovative approach to the fabrication of bone biomaterials mimicking natural bone. Bone mineral hydroxylapatite (HAP) preferentially oriented with c-axis parallel collagen fibers in However, such orientation control not easy achieve artificial biomaterials. To overcome lack control, we fabricated a phage-HAP composite by genetically engineering M13 phage, nontoxic bionanofiber, two HAP-nucleating peptides derived from one noncollagenous proteins, Dentin...
Abstract Bispecific antibodies (BsAb) have proven to be useful targeting vectors for pretargeted radioimmunotherapy (PRIT). We sought overcome key PRIT limitations such as high renal radiation exposure and immunogenicity (e.g., of streptavidin–antibody fusions), advance clinical translation this strategy diasialoganglioside GD2-positive [GD2(+)] tumors. For purpose, an IgG-scFv BsAb was engineered using the sequences anti-GD2 humanized monoclonal antibody hu3F8 C825, a murine scFv with...
Combining biodegradable materials with bioactive factors for skin wound healing has been receiving significant attention. This study was inspired by the adhesion mechanism of a marine organism, mussel. 3,4-Dihydroxyphenylalanine (DOPA) introduced to basic fibroblast growth factor (bFGF) and ponericin G1 (PonG1) using tyrosine hydroxylation, which are expected have strong binding affinities surfaces materials. DOPA-bFGF DOPA-PonG1 were applied surface modification patterned...
Background T cell-based immunotherapies using chimeric antigen receptors (CAR) or bispecific antibodies (BsAb) have produced impressive responses in hematological malignancies. However, major hurdles remained, including cytokine release syndrome, neurotoxicity, on-target off-tumor effects, reliance on autologous cells, and failure most solid tumors. BsAb armed cells offer a safe alternative. Methods We generated ex vivo (EATs) IgG-[L]-scFv-platformed BsAb, where the anti-CD3 (huOKT3) scFv...
In the tumor microenvironment (TME), myeloid cells play a pivotal role. Myeloid-derived immunosuppressive cells, including tumor-associated macrophages (TAMs) and myeloid-derived suppressor (MDSCs), are central components in shaping milieu of tumor. Within TME, majority TAMs assume an M2 phenotype, characterized by their pro-tumoral activity. These promote cell growth, angiogenesis, invasion, migration. contrast, M1 macrophages, under appropriate activation conditions, exhibit cytotoxic...
Abstract Background EGFR and/or HER2 expression in pancreatic cancers is correlated with poor prognoses. We generated homodimeric (EGFRxEGFR or HER2xHER2) and heterodimeric (EGFRxHER2) T cell-engaging bispecific antibodies (T-BsAbs) to direct polyclonal cells these antigens on tumors. Methods T-BsAbs were constructed using the 2 + IgG-[L]-scFv format bearing two anti-CD3 scFvs attached light chains of an IgG engage while retaining bivalent binding tumor both Fab arms. A arm exchange strategy...
B7-H3 and B7-H4 belong to the peripheral membrane protein B7 family are hypothesized regulate immunity. These proteins expressed in human pancreatic cancer (PC), but their prognostic significance is poorly understood. The present study examined association between B7‑H3 B7‑H4 expression overall survival time patients with PC that underwent surgery at Second Affiliated Hospital Zhengzhou University April 2000 January 2009. Immunohistochemical analysis demonstrated were 35 (88%) 30 (75%) tumor...
Radioimmunotherapy of solid tumors using antibody-targeted radionuclides has been limited by low therapeutic indices (TIs). We recently reported a novel 3-step pretargeted radioimmunotherapy (PRIT) strategy based on glycoprotein A33 (GPA33)-targeting bispecific antibody and small-molecule radioactive hapten, complex 177Lu S-2-(4-aminobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (177Lu-DOTA-Bn), that leads to high TIs for radiosensitive tissues such as blood (TI = 73) kidney 12)....
Antibody-based immunotherapy has proven efficacy for patients with high-risk neuroblastoma. However, despite being the most efficient tumoricidal effectors, T cells are underutilized because they lack Fc receptors. Using a monovalent single-chain fragment (ScFv) platform, we engineered tandem scFv bispecific antibodies (BsAbs) that specifically target disialoganglioside (GD2) on tumor and CD3 cells. Structural variants of BsAbs were constructed ranked based binding to GD2, competency in...
Gastric cancer is a common malignant tumor. Studies from our laboratory or others have shown that long non-coding RNA (lncRNA) zinc finger antisense (ZFAS)1 often acts as an oncogene. However, the molecular underpinnings of how ZFAS1 regulates gastric remain to be elucidated. Results showed expression was upregulated, and microRNA-200b-3p (miR-200b) downregulated in tissues. MiR-200b overexpression suppressed proliferation, cell cycle process, Wnt/β-catenin signaling cells. Subsequently, we...
Abstract Despite progress in the treatment of colorectal cancer, curing metastatic cancer remains a major unmet medical need worldwide. Here, we describe T-cell–engaging bispecific antibody (T-BsAb) to redirect polyclonal cytotoxic T cells eradicate cancer. A33, murine specific for GPA33, was humanized huA33 and reformatted huA33-BsAb, based on novel IgG(L)–scFv platform by linking anti-CD3 huOKT3 scFv carboxyl end light chain. This T-BsAb stably expressed CHO purified as stable monomer...
This is the initial report of an α-based pre-targeted radioimmunotherapy (PRIT) using 225Ac and its theranostic pair, 111In. We call our novel tumor-targeting DOTA-hapten PRIT system "proteus-DOTA" or "Pr." Herein we first results radiochemistry development, radiopharmacology, stoichiometry tumor antigen binding, including role specific activity, anti-tumor efficacy, normal tissue toxicity with Pr-PRIT approach (as α-DOTA-PRIT). A series α-DOTA-PRIT therapy studies were performed in three...
In recent reports, we have shown that optimized pretargeted radioimmunotherapy (PRIT) based on molecularly engineered antibody conjugates and 177 Lu-DOTA chelate (DOTA-PRIT) can be used to cure mice bearing human solid tumor xenografts using antitumor antibodies minimally internalizing membrane antigens, GPA33 (colon) GD2 (neuroblastoma).However, many antigens are internalized after binding it is generally believed not suitable targets for PRIT.In this study, tested the hypothesis DOTA-PRIT...