A5053176611- Microbial Natural Products and Biosynthesis
- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Carbohydrate Chemistry and Synthesis
- Marine Sponges and Natural Products
- Legume Nitrogen Fixing Symbiosis
- Genomics and Phylogenetic Studies
- Advanced Synthetic Organic Chemistry
- Bacterial Genetics and Biotechnology
- Chemical synthesis and alkaloids
- Plant Disease Resistance and Genetics
- Bacterial biofilms and quorum sensing
- Enzyme Catalysis and Immobilization
- Synthetic Organic Chemistry Methods
- Traditional and Medicinal Uses of Annonaceae
- Antibiotic Resistance in Bacteria
- Seaweed-derived Bioactive Compounds
- Chromatography in Natural Products
- Phytochemistry and biological activities of Ficus species
- Marine Biology and Environmental Chemistry
- Marine Ecology and Invasive Species
- Soft tissue tumor case studies
- Sarcoma Diagnosis and Treatment
- Nitrogen and Sulfur Effects on Brassica
- Phytochemical compounds biological activities
Mubarak Al Kabeer Hospital
2023-2025
University of Warwick
2016-2024
Taronga Conservation Society Australia
2024
The University of Sydney
2023-2024
Newcastle University
2020-2022
Coventry (United Kingdom)
2019
Griffith University
2014-2019
Shahid Beheshti University
2010-2013
Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis RV163, were grown in co-culture the presence of induced metabolites monitored by 1H NMR. Ten known compounds, including angucycline, diketopiperazine β-carboline derivatives 1–10, isolated from EtOAc extracts RV163. Co-cultivation RV163 biosynthesis three natural products that not detected single culture either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12)...
Burkholderia gladioli is a bacterium with broad ecology spanning disease in humans, animals and plants, but also encompassing multiple beneficial interactions. It plant pathogen, toxin-producing food-poisoning agent, causes lung infections people cystic fibrosis (CF). Contrasting traits include antifungal production exploited by insects to protect their eggs, protective abilities antibiotic biosynthesis. We explored the genomic diversity specialized metabolic potential of 206 B. strains,...
Multiple eruptive dermatofibromas (MEDF) is a rare dermatological condition characterized by the sudden appearance of multiple benign skin lesions, often associated with systemic or autoimmune conditions. This case series describes three female patients MEDF, highlighting their distinct clinical features and associations. Diagnoses were confirmed through consistent histopathological findings, demonstrating importance integrating clinical, dermoscopic, evaluations in diagnosing MEDF. The...
Fungus-associated<italic>Burkholderia gladioli</italic>bacteria use a unique ‘dual-priming’ nonribosomal peptide synthetase to assemble icosalide A1.
Abstract Inhibition of bacterial cell wall synthesis by antibiotics such as β-lactams is thought to cause explosive lysis through loss integrity. However, recent studies on a wide range bacteria have suggested that these also perturb central carbon metabolism, contributing death via oxidative damage. Here, we genetically dissect this connection in Bacillus subtilis perturbed for synthesis, and identify key enzymatic steps upstream downstream pathways stimulate the generation reactive oxygen...
The metabolite profiles of three sponge-derived actinomycetes, namely, Micromonospora sp. RV43, Rhodococcus RV157, and Actinokineospora EG49 were investigated after elicitation with N-acetyl-d-glucosamine. 1H NMR fingerprint methodology was utilized to study the differences in metabolic bacterial extracts before elicitation. Our found that addition N-acetyl-d-glucosamine modified secondary actinomycete isolates. N-Acetyl-d-glucosamine induced production 3-formylindole (11) guaymasol (12)...
A chemoinformatic method was developed to extract nonflat scaffolds embedded in natural products within the Dictionary of Natural Products (DNP). The cedrane scaffold then chosen as an example a that directs substituents three-dimensional (3D) space. has three orthogonal handles allow generation 1D, 2D, and 3D libraries synthesized on large scale. These would cover more than 50% diversity with scaffold. Synthesis focused product-like based achieved. phenotypic assay used test biological...
Abstract A gene cluster encoding a cryptic trans‐acyl transferase polyketide synthase (PKS) was identified in the genomes of Burkholderia gladioli BCC0238 and BCC1622, both isolated from lungs cystic fibrosis patients. Bioinfomatics analyses indicated PKS assembles novel member glutarimide class antibiotics, hitherto only Streptomyces species. Screening range growth parameters led to identification gladiostatin, metabolic product PKS. NMR spectroscopic analysis revealed that which has...
Thiopeptides are ribosomally biosynthesized and post-translationally modified peptides (RiPPs) that potently inhibit the growth of Gram-positive bacteria by targeting multiple steps in protein biosynthesis. The poor pharmacological properties thiopeptides, particularly their low aqueous solubility, has hindered development into clinically useful antibiotics. Antimicrobial activity screens a library Actinomycetota extracts led to discovery novel polyglycosylated thiopeptides persiathiacins A B from
Two Burkholderia gladioli strains isolated from the lungs of cystic fibrosis patients were found to produce unusual lipodepsipeptides containing a unique citrate-derived fatty acid and rare dehydro-β-alanine residue. The gene cluster responsible for their biosynthesis was identified by bioinformatics insertional mutagenesis. In-frame deletions enzyme activity assays used investigate functions several proteins encoded biosynthetic cluster, which in genomes about 45 % B. isolates, suggesting...
Response surface methodology was used to optimize conditions for lead (Pb2+) and nickel (Ni2+) biosorption from aqueous solutions, using Cystoseira trinodis (brown algae). The experiments are carried out in a batch system with 0.2 g of biomass per batch. variables as pH, initial metal concentration, contact time were optimized the critical components. A central composite rotatable design experimental analysis results. data uptake ions best described by quadratic models proposed. Using these...
Discovery of environmentally safe agents is currently considerable interest, due to the continuous impacts biofoulers on marine habitats and adverse effects biocides environment. This study reports anti-adhesion effect living Actinobacteria against fouling strains isolated from submerged panels in environments Iran. The extract Glycomyces sediminimaris affected biofilm formation Kocuria sp. Mesorhizibium sp., as dominant this ecosystem, up 93.2% 71.4%, respectively. metabolic activity fouler...
A genomic and bioactivity informed analysis of the metabolome extremophile Amycolatopsis sp. DEM30355 has allowed for discovery isolation polyketide antibiotic tatiomicin. Identification biosynthetic gene cluster was confirmed by heterologous expression in Streptomyces coelicolor M1152. Structural elucidation, including absolute stereochemical assignment, performed using complementary crystallographic, spectroscopic computational methods. Tatiomicin shows activity against Gram-positive...
Covering: up to the end of 2022In recent years rare Actinobacteria have become increasingly recognised as a rich source novel bioactive metabolites.
Abstract Background Mithramycin is an anti-tumor compound of the aureolic acid family produced by Streptomyces argillaceus . Its biosynthesis gene cluster has been cloned and characterized, several new analogs with improved pharmacological properties have generated through combinatorial biosynthesis. To further study these compounds as potential anticancer drugs requires their production yields to be significantly. The mithramycin proceeds formation key intermediate...
Growth of most rod-shaped bacteria is accompanied by the insertion new peptidoglycan into cylindrical cell wall. This insertion, which helps maintain and determine shape cell, guided a protein machine called rod complex or elongasome. Although proteins in this are essential under normal growth conditions, viability can be rescued, for reasons that not understood, presence high (mM) Mg 2+ concentration. We screened natural product compounds could rescue mutants affected rod-complex function....
Persiathiacin A is a novel thiopeptide antibiotic produced by
Plants and microbes are closely associated with each other in their ecological niches. Much has been studied about plant-microbe interactions, but little is known the effect of phytochemicals on at molecular level. To access products cryptic biosynthetic gene clusters bacteria, we incorporated an organic extract hibiscus flowers into culture media different Actinobacteria isolated from plant rhizospheres. This approach led to production broad-spectrum dithiolopyrrolone (DTP) antibiotics,...
Burkholderia are Gram-negative bacteria with varied lifestyles and versatile phenotypes including the production of bioactive natural products [1]. gladioli has previously been shown to produce secondary metabolites such as bongkrekic acid [2] toxoflavin [3]. To fully understand potential B. novel products, a collection 88 isolates was genome-sequenced using Illumina technology; reference 8.4 Mb genome produced single molecule real time sequencing. Genomic analysis revealed wealth metabolite...
Solitary trichoepitheliomas (TE) are benign tumors that strikingly similar to their malignant counterpart, basal cell carcinoma (BCC). An 83-year-old man presented with a 10-year history of right lower lid skin mass initially diagnosed as BCC. Intraoperatively, an excisional biopsy was performed primary reconstruction the defect and specimen submitted for histopathology processing. Eventually, findings suggested diagnosis hair follicle tumor. The postoperative results were aesthetically...