A5080767202- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Marine Sponges and Natural Products
- Lichen and fungal ecology
- Fungal Biology and Applications
- Biocrusts and Microbial Ecology
- Chromatography in Natural Products
- Advanced Chemical Sensor Technologies
- Microbial Metabolism and Applications
- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Analytical Chemistry and Chromatography
- Vanadium and Halogenation Chemistry
- Plant Pathogens and Fungal Diseases
- Microbial Community Ecology and Physiology
- Computational Drug Discovery Methods
- Marine Toxins and Detection Methods
- Protist diversity and phylogeny
- Antifungal resistance and susceptibility
- Mycotoxins in Agriculture and Food
- Alkaloids: synthesis and pharmacology
- Plant biochemistry and biosynthesis
- Genomics and Phylogenetic Studies
- Chemical synthesis and alkaloids
- Crystallization and Solubility Studies
Nantes Université
2013-2024
Institut Des Substances et Organismes de la Mer
2023
Sookmyung Women's University
2020
Université Nantes Angers Le Mans
2017
Université Bretagne Loire
2017
Griffith University
2013-2015
Université de Rennes
2008-2011
Centre National de la Recherche Scientifique
2011
Université Européenne de Bretagne
2009-2010
Institut des Sciences Chimiques de Rennes
2006
Maitotoxins (MTXs) are among the most potent toxins known. These produced by epi-benthic dinoflagellates of genera
A collection of culture extracts obtained from several marine-derived fungal strains collected on the French Atlantic coast was investigated by high performance liquid chromatography–high resolution mass spectrometry (HPLC-HRMS) in order to prospect for halogenated compounds and identify potentially new ones. To achieve a fast, automated, efficient data analysis, bioinformatics tool named MeHaloCoA (Marine Halogenated Compound Analysis) developed included into R. After extraction all peaks...
Mycosporine-like amino acids (MAAs) represent a suite of small molecules which have unique ultraviolet-absorbing capacities, based on their common cyclohexenone or cyclohexenimine conjugated arrangements. MAAs also strong antioxidant properties, and participate in the osmotic equilibrium numerous diverse marine organisms that produce them, especially photosymbiotic partnerships. In this chapter historical introduction is provided to discovery MAAs, together with details primary secondary...
The discovery of new natural products from fungi isolated the marine environment has increased dramatically over last few decades, leading to identification 1000 metabolites. However, most reported marine-derived species appear be terrestrial in origin yet at same time, facultatively halo- or osmotolerant. An unanswered question regarding apparent chemical productivity is whether common practice fermenting strains seawater contributes enhanced secondary metabolism? To answer this question, a...
Fragment-based screening is commonly used to identify compounds with relatively weak but efficient localized binding protein surfaces. We mass spectrometry study fragment-sized three-dimensional natural products. identified seven securinine-related Plasmodium falciparum 2′-deoxyuridine 5′-triphosphate nucleotidohydrolase (PfdUTPase). Securinine bound allosterically PfdUTPase, enhancing enzyme activity and inhibiting viability of both P. gametocyte (sexual) blood (asexual) stage parasites....
Penicillium ubiquetum MMS330 isolated from the blue mussel Mytilus edulis collected on Loire estuary in France was here investigated. As very few secondary metabolites have been documented for this species, its metabolome studied following OSMAC approach to enhance as many biosynthetic pathways possible. Interestingly, HPLC-HRMS based hierarchical clustering analysis together with MS/MS molecular networking highlighted selective overproduction of some structurally related compounds when...
This work aimed at studying metabolome variations of marine fungal strains along their growth to highlight the importance parameter “time” for new natural products discovery. An untargeted time-scale metabolomic study has been performed on two different marine-derived Penicillium strains. They were cultivated 18 days and crude extracts analyzed by HPLC-DAD-HRMS (High Performance Liquid Chromatography-Diode Array Detector-High Resolution Mass Spectrometry) each day. With example griseofulvin...
Abstract Communesins are rare alkaloids isolated from fungi of the genus Penicillium . In this work, extract a marine‐derived expansum strain was studied using targeted molecular networking approach allowing to detect 65 communesins including 55 new ones. A fragmentation pattern for dimethylvinyl established and script implemented predict structure map all in global network. semisynthetic strategy carried out obtain some minor congeners two B. Nine were then synthetised: them already...
In the course of investigations on marine-derived toxigenic fungi, five strains Trichoderma atroviride were studied for their production peptaibiotics. While these found to produce classical 19-residue peptaibols, three them exhibited unusual peptidic sodium-adduct [M + 2 Na](2+) ion peaks at m/z between 824 and 854. The sequencing peptides led two series unprecedented 17-residue peptaibiotics based model Ac-XXX-Ala-Ala-XXX-XXX-Gln-Aib-Aib-Aib-Ala/Ser-Lxx-Aib-Pro-XXX-Aib-Lxx-[C(129) ]....
An optimized nitroso-based probe that facilitates the discovery of conjugated alkene-containing natural products in unprocessed extracts was developed. It chemoselectively reacts with olefins via a nitroso-Diels-Alder cyclization to yield derivatives distinct chromophore and an isotopically unique bromine atom can be rapidly identified using liquid chromatography/mass spectrometry bioinformatics tool called MeHaloCoA (Marine Halogenated Compound Analysis). The is ideally employed when...
Herein, we present a protocol for the use of Global Natural Products Social (GNPS) Molecular Networking, an interactive online chemistry-focused mass spectrometry data curation and analysis infrastructure. The goal GNPS is to provide as much chemical insight untargeted tandem set possible connect this underlying biological questions user wishers address. This can be performed within one experiment or at repository scale. not only serves public with sample information (metadata), it also...
Abstract Early research on marine fungi was mostly descriptive, with an emphasis their diversity and taxonomy, especially of those collected at rocky shores seaweeds driftwood. Subsequently, further substrata (e.g. salt marsh grasses, animals, seagrasses, sea foam, seawater, sediment) habitats (coral reefs, deep-sea, hydrothermal vents, mangroves, sandy beaches, marshes) were explored for fungi. In parallel, areas have broadened from micro-morphology to ultrastructure, ecophysiology,...
The initiation of this study relies on a targeted genome-mining approach to highlight the presence putative vanadium-dependent haloperoxidase-encoding gene in deep-sea hydrothermal vent fungus Hortaea werneckii UBOCC-A-208029. To date, only three fungal haloperoxidases have been described, one from terrestrial species Curvularia inaequalis, plant pathogen Botrytis cinerea, and marine derived isolate identified as Alternaria didymospora. In study, we describe new vanadium chloroperoxidase...