A5016985612- Marine Sponges and Natural Products
- Microbial Natural Products and Biosynthesis
- Trypanosoma species research and implications
- Aquaculture disease management and microbiota
- Research on Leishmaniasis Studies
- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Genomics and Phylogenetic Studies
- Coral and Marine Ecosystems Studies
- Traditional and Medicinal Uses of Annonaceae
- Antimicrobial Peptides and Activities
- Bioactive Compounds and Antitumor Agents
- Identification and Quantification in Food
- Synthesis and Biological Activity
- Essential Oils and Antimicrobial Activity
- Amphibian and Reptile Biology
- Bacterial Identification and Susceptibility Testing
- Insect and Arachnid Ecology and Behavior
- Marine Invertebrate Physiology and Ecology
- Insect symbiosis and bacterial influences
- Alkaloids: synthesis and pharmacology
- Plant biochemistry and biosynthesis
- Phytochemical Studies and Bioactivities
- Microbial Metabolic Engineering and Bioproduction
- Species Distribution and Climate Change
City of Knowledge
2011-2025
Instituto de Investigaciones Científicas y Servicios de Alta Tecnología
2014-2025
Secretaría Nacional de Ciencia, Tecnología e Innovación
2025
Acharya Nagarjuna University
2019
University of Montana
2009-2011
Scripps Institution of Oceanography
2008-2010
University of Freiburg
2010
University of California, San Diego
2009
University of California, Santa Cruz
2009
University of Papua New Guinea
2008
The ability to correlate the production of specialized metabolites genetic capacity organism that produces such molecules has become an invaluable tool in aiding discovery biotechnologically applicable molecules. Here, we accomplish this task by matching molecular families with gene cluster families, making these correlations 60 microbes at one time instead connecting molecule a time, as how it is traditionally done. We can through use nanospray desorption electrospray ionization MS/MS,...
Cancer cell toxicity-guided fractionation of extracts the Papua New Guinea marine cyanobacteria Lyngbya majuscula and sordida led to isolation apratoxin D (1). Compound 1 contains same macrocycle as apratoxins A C but possesses novel 3,7-dihydroxy-2,5,8,10,10-pentamethylundecanoic acid polyketide moiety. The planar structures stereostructures compound were determined by extensive 1D 2D NMR MS data analyses comparison with spectroscopic C. Apratoxin (1) showed potent in vitro cytotoxicity...
The fungus-growing ant-microbe symbiosis is an ideal system to study chemistry-based microbial interactions due the wealth of described, and lack information on molecules involved therein. In this study, we employed a combination MALDI imaging mass spectrometry (MALDI-IMS) MS/MS molecular networking in system. IMS was used visualize distribution antimicrobials at inhibition zone between bacteria associated ant Acromyrmex echinatior fungal pathogen Escovopsis sp. for dereplication compounds...
Natural and non-natural cyclic peptides are a crucial component in drug discovery programs because of their considerable pharmaceutical properties. Cyclosporin, microcystins, nodularins all notable pharmacologically important peptides. Because these biologically active often biosynthesized nonribosomally, they contain nonstandard amino acids, thus increasing the complexity resulting tandem mass spectrometry data. In addition, nature, fragmentation patterns many showed much higher when...
Coral reefs are intricate ecosystems that harbor diverse organisms, including 25% of all marine fish. Healthy corals exhibit a complex symbiosis between coral polyps, endosymbiotic alga, and an array microorganisms, called the holobiont. Secretion specialized metabolites by microbiota is thought to contribute defense this sessile organism against harmful biotic abiotic factors. While few causative agents diseases have been unequivocally identified, fungi implicated in massive destruction...
A family of 2,2-dimethyl-3-hydroxy-7-octynoic acid (Dhoya)-containing cyclic depsipeptides, named dudawalamides A–D (1–4), was isolated from a Papua New Guinean field collection the cyanobacterium Moorea producens using bioassay-guided and spectroscopic approaches. The planar structures were determined by combination 1D 2D NMR experiments MS analysis, whereas absolute configurations X-ray crystallography, modified Marfey's chiral-phase GCMS, HPLC. Dudawalamides possess broad spectrum...
La relevancia de Salmonella como patógeno alimentario es sobra conocida. microbiota intestinal una las primeras líneas defensa contra la infección por estas bacterias entéricas, lo que conocer cómo interaccionan estos patógenos con clave para comprender mejor este proceso invasión e y desarrollar posibles estrategias su prevención. Por ello en trabajo se estudió influencia distintos grupos/ especies microbianas tienen capacidad invadir el epitelio (simulado células Caco-2). Los resultados...
Fractionation of the extract marine cyanobacterium Lyngbya majuscula collected from Panama led to isolation malyngolide dimer (1). The planar structure 1 was determined using 1D and 2D NMR spectroscopy HRESI-TOFMS. absolute configuration established by chemical degradation followed chiral GC-MS analyses comparisons with an authentic sample seco-acid (4). Compound showed moderate in vitro antimalarial activity against chloroquine-resistant Plasmodium falciparum (W2) (IC(50) = 19 microM) but...
Fractionation of the ethyl acetate extract marine bacterium Bacillus pumilus isolated from black coral Antipathes sp. led to isolation five compounds: cyclo-(L-Leu-L-Pro) (1), 3-hydroxyacetylindole (2), N-acetyl-b-oxotryptamine (3), cyclo-(L-Phe-L-Pro) (4), and 3-formylindole (5). The structures compounds 1−5 were established by spectroscopic analyses, including HRESITOF-MS NMR (1H, 13C, HSQC, HMBC COSY). Compounds 2, 3 5 caused inhibition on growth Trypanosoma cruzi (T. cruzi), with IC50...
Bioassay-guided fractionation of the MeOH extract from octocoral Muricea austera collected in Pacific coast Panama led to isolation eight compounds, including three tyramine derivatives (1−3), two steroidal pregnane glycosides (4, 5), and sesquiterpenoids (6−8). Compounds 2−5 are new natural products, their structures were determined on basis spectroscopic data (HRMS, 1D 2D NMR, CD studies). The antiprotozoal activities compounds 1−8 as well those a series synthetic (11−22) (23−35) evaluated...
Tanikolide seco-acid 2 and tanikolide dimer 3, the latter a novel selective SIRT2 inhibitor, were isolated from Madagascar marine cyanobacterium Lyngbya majuscula. The structure of 2, as pure R enantiomer, was elucidated by X-ray experiment in conjunction with NMR optical rotation data, whereas depside molecular 3 initially thought to be meso compound established NMR, MS, chiral HPLC analyses. Subsequent total synthesis three stereoisomers 4, 5, ent-5, followed GC−MS comparisons natural...
NMR-guided fractionation of two independent collections the marine cyanobacteria Lyngbya majuscula obtained from Papua New Guinea and Oscillatoria sp. collected in Panama led to isolation new lipids serinolamide A (3) propenediester (4). Their structures were determined by NMR MS data analysis. Serinolamide exhibited a moderate agonist effect selectivity for CB1 cannabinoid receptor (Ki = 1.3 μM, >5-fold) represents newest addition known cannabinomimetic natural products origin.
Alzheimer's disease (AD) is the most common neurodegenerative disorder affecting elderly population worldwide. Brain inflammation plays a key role in progression of AD. Deposition senile plaques brain stimulates an inflammatory response with overexpression pro-inflammatory mediators, such as neuroinflammatory cytokine. interleukin-6. Curcumin has been revealed to be potential agent for treating AD following different neuroprotective mechanisms, inhibition aggregation and decrease...
Abstract Amphibian populations worldwide have declined and in some cases become extinct due to chytridiomycosis, a pandemic disease caused by the fungus Batrachochytrium dendrobatidis ; however, species survived these fungal epidemics. Previous studies suggested that resistance of is presence cutaneous bacteria producing antifungal metabolites. As our understanding metabolites still limited, we assessed potential such compounds against human-relevant fungi as Aspergillus . In this work...
Six furanocembranolides (1−6) and one pseudopterolide (7) have been isolated from the octocorals Leptogorgia alba rigida, collected on Pacific coast of Panama. Compound 1, named leptolide, has a new structure closely related to neurotoxin lophotoxin (3). The X-ray structures 1−3 were determined, absolute configurations 2−7 are discussed. Compounds 1−7 evaluated in vitro against drug-resistant Plasmodium falciparum.
The present research aimed to evaluate the antibacterial activity of volatile organic compounds (VOCs) produced by octocoral-associated bacteria Bacillus sp. BO53 and Pseudoalteromonas GA327. volatilome bioactivity both species was evaluated against human pathogenic antibiotic-resistant bacteria, methicillin-resistant Staphylococcus aureus, Acinetobacter baumanni, Pseudomonas aeruginosa. In this regard, in vitro tests showed that VOCs inhibited growth P. aeruginosa reduced S. aureus A....
From the aereal part of Salvi a ballotaeflora, Benth (Lamiaceae) three new diterpene quinones were isolated, all with an abietane skeleton. One them, romulogarzone is ortho-quinone, other two, conacytone and icetexone are para–quinones, their structures elucidated on chemical physical data.
Pseudoalteromonas is a genus of marine bacteria often found in association with other organisms. Although several studies have examined diversity and their antimicrobial activity, its tropical environments largely unexplored. We investigated the Panama using multilocus phylogenetic approach. Furthermore we tested capacity evaluated effect recombination mutation shaping relationships. The reconstruction clonal relationships among 78 strains including 15 reference species revealed 43 lineages,...
Herein, we present a protocol for the use of Global Natural Products Social (GNPS) Molecular Networking, an interactive online chemistry-focused mass spectrometry data curation and analysis infrastructure. The goal GNPS is to provide as much chemical insight untargeted tandem set possible connect this underlying biological questions user wishers address. This can be performed within one experiment or at repository scale. not only serves public with sample information (metadata), it also...