The current emergency due to the worldwide spread of COVID-19 caused by new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a great concern for global public health. Already in past, outbreak (SARS) 2003 and Middle Eastern (MERS) 2012 demonstrates potential coronaviruses cross-species borders further underlines importance identifying new-targeted drugs. An ideal antiviral agent should target essential proteins involved lifecycle SARS-CoV. Currently, some HIV protease...

Due to their antimicrobial and antiviral activity potential in vitro, polyphenols are gaining a lot of attention from the pharmaceutical healthcare industries. A novel approach could be based on use obtained natural sources. Here, we tested antibacterial effect mix present almond skin (NS MIX). The was evaluated against standard American Type Culture Collection (ATCC) clinical strains Staphylococcus aureus, including methicillin-resistant (MRSA) strains, by minimum inhibitory concentration...

The current emergency due to the worldwide spread of COVID-19 caused by new SARS-CoV-2 is a great concern for global public health. Already in past, outbreak severe acute respiratory syndrome (SARS) 2003 and Middle Eastern (MERS) 2012 demonstrates potential coronaviruses cross-species borders further underlines importance identifying new-targeted drugs. An ideal antiviral agent should target essential proteins involved lifecycle SARS-CoV. Currently, some HIV protease inhibitors (i.e.,...

The present study aims to assess the antioxidant and antiviral effectiveness of leaf extracts obtained from Olea europaea L. var. sativa sylvestris. total activity was determined via both an ammonium phosphomolybdate assay a nitric oxide radical inhibition assay. Both showed reducing abilities in vitro system human HeLa cells. Indeed, after oxidative stress induction, we found that exposition olive protects cells lipid peroxidation increases concentration enzyme antioxidants such as catalase...

The topic dealt with in this paper concerns an experimental study, performed by means of ultrasonic techniques, on aqueous solutions α,α-trehalose, effective bioprotector against dehydration. velocity and density data show that, these solutions, the mixing process is not ideal. behavior excess compressibility as a function concentration can be interpreted supposing that α,α-trehalose molecules form intramolecular hydrogen bonds folding over at high concentration. When water are added, they...

An earlier report has shown that herpes simplex virus 1 virions package RNA. Experiments designed to reveal the identity of virion proteins capable binding RNA and show whether mRNA carried in newly infected cells was expressed showed following: (i) (32)P-labeled riboprobe generated by vitro transcription U(S)8.5 ORF bound three identified as products U(S)11, U(L)47, U(L)49 (VP22) genes. (ii) Viral U(L)47 or U(S)11 immune precipitated from transduced with baculoviruses expressing then...

The aim of the present research was to determine effect almond skin extracts on herpes simplex virus 1 (HSV-1) replication. Drug-resistant strains HSV frequently develop following therapeutic treatment. Therefore, discovery novel anti-HSV drugs deserves great effort. Here, we tested both natural (NS) and blanched (BS) polyphenols-rich against HSV-1. HPLC analysis showed that prevalent compounds in NS BS contributing their antioxidant activity were quercetin, epicatechin catechin. Results...

In the development of new antibacterial therapeutic approaches to fight multidrug-resistant bacteria, antimicrobial photodynamic therapy (aPDT) represents a well-known alternative treat local infections caused by different microorganisms. Here we present polypropylene (PP) fabric finished with citrate-hydroxypropyl-βCD polymer (PP-CD) entrapping tetra-anionic 5,10,15,20-tetrakis(4-sulfonatophenyl)-21H,23H-porphine (TPPS) as photosensitizer-eluting scaffold (PP-CD/TPPS) for aPDT. The concept...

Since the onset of COVID-19 (COronaVIrus Disease 19) pandemic, SARS-CoV-2 has exhibited a high transmission rate, further enhanced by new variants able to better adapt humans. Addressing this issue been challenging due viral resistance and side effects associated with antiviral drugs vaccines. As result, there growing interest in plant-derived compounds properties. Our study revealed that pistachio extracts significantly inhibited entry. Employing pseudotyped particles bearing S protein...

Signals involved in protection against apoptosis by herpes simplex virus 1 (HSV-1) were investigated. Using U937 monocytoid cells as an experimental model, we have demonstrated that HSV-1 rendered these resistant to Fas-induced promptly after infection. UV-inactivated well the envelope glycoprotein D (gD) of HSV-1, itself, exerted a protective effect on apoptosis. NF-κB was activated gD, and Fas-mediated gD abolished stably transfected with dominant negative mutant I-κBα, indicating...

ABSTRACT Following the lead of recent studies on presence RNA in virions human cytomegalovirus, we investigated and identity RNAs from purified herpes simple virus 1. To facilitate these studies, designed primers for all known open reading frames (ORFs) also constructed cDNA arrays containing probes to detect transcripts. In first series experiments, labeled DNA made by reverse transcription poly(A) + extracted infected HEp-2 or rabbit skin cells hybridized but two array. A similar analysis...

Phosphonated carbocyclic 2'-oxa-3'-aza-nucleosides have been synthesized in good yields by 1,3-dipolar cycloaddition methodology. The cytotoxicity and the reverse transcriptase inhibitory activity of obtained compounds investigated. 2'-oxa-3'-aza-nucleosides, while showing low levels cytotoxicity, exert a specific inhibitor on two different transcriptases, which is comparable with that AZT, opening new perspectives their possible use as therapeutic agents, anti-retroviral anti-HBV chemotherapy.

The virion host shutoff (vhs) protein encoded by the U L 41 gene of herpes simplex virus 1 is an endoribonuclease. enzyme introduced into cell during unpackaging upon entry and selectively degrades mRNA for several hours. RNase activity ceases after onset synthesis late (γ) viral proteins. Here we report that vhs does not accumulate in cells transiently transfected with only a plasmid encoding gene. However, cotransfected plasmids expressing two other tegument proteins, VP16 VP22. directly...

Abstract We have developed a supramolecular nanoassembly capable of inducing remarkable levels cancer cell mortality through bimodal action based on the simultaneous photogeneration nitric oxide (NO) and singlet oxygen ( 1 O 2 ). This was achieved appropriate incorporation an anionic porphyrin (as photosensitizer) tailored NO photodonor in different compartments biocompatible nanoparticles cationic amphiphilic cyclodextrins. The combination steady‐state time‐resolved spectroscopic techniques...

Abstract Autophagy is a cellular degradation pathway that exerts numerous functions in vital biological processes. Among these, it contributes to both innate and adaptive immunity. On the other hand, pathogens have evolved strategies manipulate autophagy for their own advantage. By monitoring autophagic markers, we showed HSV-1 transiently induced autophagosome formation during early times of infection monocytic THP-1 cells human monocytes. by mechanism independent viral gene expression or...

Natural compounds are a prominent source of novel antiviral drugs. Several reports have previously shown the antimicrobial activity pistachio polyphenol extracts. Therefore, aim our research was to investigate polyphenol-rich extracts natural shelled (NPRE) pistachios kernels (Pistacia vera L.) on herpes simplex virus type 1 (HSV-1) replication. The Vero cell line used assess cytotoxicity and activity. viability calculated by detection cellular ATP after treatment with various concentrations...

The synthesis of α-fluorinated methyl ketones has always been challenging. New methods based on the homologation chemistry via nucleophilic halocarbenoid transfer, carried out recently in our labs, allowed us to design and synthesize a target-directed dipeptidyl α,α-difluoromethyl ketone (DFMK) 8 as potential antiviral agent with activity against human coronaviruses. ability newly synthesized compound inhibit viral replication was evaluated by cytopathic effect (CPE)-based assay performed...