A5079153689- Forensic and Genetic Research
- Genetic diversity and population structure
- Cancer, Hypoxia, and Metabolism
- 3D Printing in Biomedical Research
- Genetic Associations and Epidemiology
- Metabolism, Diabetes, and Cancer
- Metabolomics and Mass Spectrometry Studies
- Cancer Cells and Metastasis
- Pluripotent Stem Cells Research
- FOXO transcription factor regulation
- Pancreatic function and diabetes
- Cancer, Lipids, and Metabolism
- Virus-based gene therapy research
- Congenital Heart Disease Studies
- Bioinformatics and Genomic Networks
- Neurogenesis and neuroplasticity mechanisms
- Archaeology and ancient environmental studies
- RNA regulation and disease
- Genomics and Phylogenetic Studies
- Cellular Mechanics and Interactions
- Microtubule and mitosis dynamics
- CRISPR and Genetic Engineering
- Mechanical Circulatory Support Devices
- Organ Transplantation Techniques and Outcomes
- interferon and immune responses
Moffitt Cancer Center
2023
Molecular Oncology (United States)
2023
National Cancer Institute
2022
Center for Cancer Research
2022
University of Wisconsin–Madison
2013-2022
University of Puerto Rico-Mayaguez
2010-2013
The Caribbean basin is home to some of the most complex interactions in recent history among previously diverged human populations. Here, we investigate population genetic this region by characterizing patterns genome-wide variation 330 individuals from three Greater Antilles (Cuba, Puerto Rico, Hispaniola), two mainland (Honduras, Colombia), and Native South American (Yukpa, Bari, Warao) We combine these data with a unique database genomic over 3,000 diverse European, African, use local...
BackgroundDuctal carcinoma in situ (DCIS) is the earliest stage of breast cancer. During DCIS, tumor cells remain inside mammary duct, growing under a microenvironment characterized by hypoxia, nutrient starvation, and waste product accumulation; this harsh promotes genomic instability eventually cell invasion. However, there lack biomarkers to predict what patients will transition more invasive or how DCIS manage survive microenvironment.MethodsIn work, we have developed microfluidic model...
Abstract There is a vital need to develop in vitro models of the developing human brain recapitulate biological effects that toxic compounds have on brain. To model perineural vascular plexus (PNVP) vitro, which key stage embryonic development, stem cells (hESC)‐derived endothelial (ECs), neural progenitor cells, and microglia (MG) with primary pericytes (PCs) synthetic hydrogels custom‐designed microfluidics device are cocultured. The formation includes networks ECs (CD31+, VE‐cadherin+),...
Ensuring genetic stability in pluripotent stem cell (PSC) cultures is essential for the development of successful therapies. Although most instances lead to failed experiments and go unreported literature, many laboratories have found emergence abnormalities PSCs when cultured vitro prolonged amounts time. These cells are primarily non-physiological stiff substrates like tissue culture polystyrene (TCPS) which raises possibility that cause these may be influenced by substrate mechanics. In...
Breast cancer metastasis occurs via blood and lymphatic vessels. cells 'educate' endothelial (LECs) to support tumor vascularization growth. However, despite known metabolic alterations in breast cancer, it remains unclear how cell metabolism is altered the microenvironment its effect lymphangiogenic signaling LECs. We analyzed metabolites inside LECs co-culture with MCF-7, MDA-MB-231, SK-BR-3 lines using [Formula: see text] nuclear magnetic resonance (NMR) metabolomics, Seahorse, spatial...
Abstract As important as the fasting response is for survival, an inability to shut it down once nutrients become available can lead exacerbated disease and severe wasting. The liver central transitions between feeding states, with glucagon being a key initiator of hepatic response. However, precise mechanisms controlling are not well defined. One potential mediator these kinase B1 (LKB1), given its role in nutrient sensing. Here, we show LKB1 knockout mice have wasting prolonged phenotype...
Abstract Breast cancer is the second most commonly diagnosed among women worldwide. Patient death typically caused by metastasis development rather than primary tumor. Metastasis in breast has been shown to occur via blood and lymphatic vessels. Research shows that cells ‘educate' endothelial support tumor growth stimulating factor secretion directing dissemination. In addition, cell metabolism altered during malignant transformation. Cancer have increased energy macromolecule biosynthesis...
Ovarian cancer frequently metastasizes to the omentum, which is primarily comprised of adipocytes. Our previous study found that sucrose nonfermenting-related kinase (SNRK) expression lower in advanced-stage compared with early-stage ovarian tissue. In this study, SNRK knockdown was performed cell lines using lentiviral transduction and resulted decreased proliferation, increased invasion, a switch metabolism fatty acid oxidation (FAO). data suggest works as metabolic checkpoint allows for...
The Caribbean basin is home to some of the most complex interactions in recent history among previously diverged human populations. Here, by making use genome-wide SNP array data, we characterize ancestral components populations on a sub-continental level and unveil fine-scale patterns population structure distinguishing insular from mainland as well other Hispanic/Latino groups. We provide genetic evidence for an inland South American origin Native component island extensive pre-Columbian...
Abstract Breast cancer is the second most commonly diagnosed among women worldwide. Patient death typically caused by metastasis development rather than primary tumor. Metastasis in breast has been shown to occur via blood and lymphatic vessels. Research shows that cells ‘educate’ endothelial support tumor growth stimulating factor secretion; specifically VEGF, PDGF-BB EGF secretion. In addition, cell metabolism altered during malignant transformation. Cancer have increased energy...
Ductal carcinoma in situ (DCIS) is the earliest stage of breast cancer. During DCIS,tumor cells remain inside mammary duct, growing under a microenvironment characterized by hypoxia, nutrient starvation, and waste product accumulation; this harsh promote genomic instability eventually cell invasion. We have developed microfluidic model that recapitulates those aspects DCIS microenvironment. In device, were grown luminal duct model, embedded 3D hydrogel with fibroblasts. metabolism led to...
Abstract As important as the fasting response is for survival, an inability to shut it down once nutrients become available can lead exacerbated disease and severe wasting. The liver central transitions between feeding states, with glucagon being a key initiator of hepatic response. However, precise mechanisms controlling are not well defined. One potential mediator these Liver Kinase B1 (LKB1) given its role in nutrient sensing. Here, we show LKB1 knockout mice have wasting prolonged...