A5062027060- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Research Data Management Practices
University of Würzburg
2020
ZB MED - Information Centre for Life Sciences
2020
RNA-protein interactions are the crucial basis for many steps of bacterial gene expression, including post-transcriptional control by small regulatory RNAs (sRNAs). In stark contrast to recent progress in analysis Gram-negative bacteria, knowledge about complexes Gram-positive species remains scarce. Here, we used Grad-seq approach draft a comprehensive landscape such Streptococcus pneumoniae, total determining sedimentation profiles ~ 88% transcripts and 62% proteins this important human...
Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism respect to global protein-protein networks. Yet, there no census RNA/protein complexes in this model species microbiology. Here, we performed Grad-seq establish an complexome, reconstructing sedimentation profiles a glycerol gradient for ∼85% all E. transcripts and ∼49% proteins. These include majority small noncoding RNAs (sRNAs)...
ABSTRACT Stable protein complexes, including those formed with RNA, are major building blocks of every living cell. Escherichia coli has been the leading bacterial organism respect to global protein-protein networks. Yet, there no census RNA/protein complexes in this model species microbiology. Here, we performed Grad-seq establish an complexome, reconstructing sedimentation profiles a glycerol gradient for ~85% all E. transcripts and ~49% proteins. These include majority small noncoding...