MicroRNAs (miRNAs) are small noncoding RNAs that play critical roles in regulating various cellular functions by transcriptional silencing. miRNAs can function as either oncogenes or tumor suppressors (oncomirs), depending on cancer types. In our study, using miRNA microarray, we observed downregulation of the Notch-1 pathway, delta-tocotrienol, correlated with upregulation miR-34a, nonsmall cell lung cells (NSCLC). Moreover, re-expression miR-34a transfection NSCLC resulted inhibition...

Pancreatic cancer (PC) patients have poor prognosis and survival rate. Gemcitabine, the drug of choice has a dismal 15% response Earlier, we reported that Garcinol alone in combination with gemcitabine showed dose-dependent favorable on PC cell lines. This study probes vivo effects dietary progression transgenic mice (KPC; K-ras p53 conditional mutant). KPC male were divided into: KC- Control diet; KGr-0.05% KGm-Gemcitabine injected; KGG – diet + Gemcitabine injected groups. Changes tumor...

Poor prognosis in Pancreatic cancer (PC) is attributable to weak diagnostic and treatment options. Surgical resection possible for 20% of diagnosed cases while current chemotherapy (Gemcitabine) has a 15% response rate. Previously, we reported that Garcinol, polyisoprenylated benzophenone, from Garcinia indica, shows favorable dose dependant PC cells, alone combination with Gemcitabine. In this study report the invivo effects dietary Garcinol transgenic pancreatic mouse model (KPC). Methods...

Previously, we reported that ProAlgaZyme (PAZ) and its biologically active fraction improved plasma lipids in hypercholesterolemic hamsters, by significantly increasing the high density lipoprotein cholesterol (HDL-C) while reducing non-HDL ratio of total cholesterol/HDL-C. Moreover, hepatic mRNA expression genes involved HDL/reverse transport were increased, cholesteryl ester transfer protein (CETP) was partially inhibited. In current study, investigated therapeutic efficacy PAZ (BaP) on...

Breast cancer is one of the most commonly diagnosed types among women worldwide. Cytochrome P450 aromatase (CYP19A1) an enzyme in vertebrates that selectively catalyzes biosynthesis estrogens from androgenic precursors. Researchers have increasingly focused on developing non-steroidal inhibitors (NSAIs) for their potential clinical use, avoiding steroidal side effects.The objective present work to search lead compounds ZINC database through various silico approaches.In study, were initially...

Pancreatic ductal adenocarcinoma (PDAC) is an extremely aggressive form of pancreatic cancer with low survival rates partly due to late diagnosis and poor treatment outcomes. The potential curative through surgical resection only limited early stages PDAC while the use chemotherapy drug, gemcitabine alone offers minimal benefits. This study aimed explore in vivo effects oil palm phenolics (OPP), a water‐soluble extract from African palm, Elaeis guineensis , transgenic mouse model PDAC....

Patients of PC have poor prognosis in part due to therapeutic response. Phytochemicals may be beneficial against cancer progression. OPP, a water‐soluble extract from African oil palm, Elaeis guineensis , has known anticancer properties. This study compared the effect OPP with gemcitabine, current drug treatment, mouse model. Methods KPC (KrasLSL.G12D/+; p53R172H/+; PdxCretg/+) mice were divided into groups: KC (control diet), KP (5% KG (gemcitabine), KPG diet + gemcitabine). Littermates...

A recent and major scientific achievement in the field of biotechnology is discovery CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats).CRISPR has become one most modern popular tools, mainly due to its low cost efficiency, which could be used edit genome.As a result, this technology holds key almost every dimension biomedical agricultural sciences, potential applications treatment viral infections, hemophilia, cancer inherited genetic anomalies.However, ethical issues crop...

Pancreatic adenocarcinoma has less than 6% five year survival rate, the lowest in all cancer types. Garcinol is a polyisoprenylated benzophenone derivative from Garcinia Indica fruit extract. Invitro data our lab showed that displayed dose dependent favorable response pancreatic lines. In this study, we investigated invivo effects of dietary transgenic mouse model Cancer. Reduced tumor progression observed MRI scans live animal was through changes micro RNA and mRNA expressions...

Cardiovascular disease (CVD) is the leading cause of death worldwide. Major risk factors for CVD, including elevated LDL and low HDL were investigated, upon ingestion AI. The lipid profile was correlated with change in plasma metabolomic over time. Fifty male Golden Syrian hamsters fed a high fat diet 4 weeks prior to randomization into 6 groups: T0, T3, T7, T10, T14, T21, received 20% AI 0, 3, 7, 10, 14, 21 days, respectively, as their drinking fluid. Plasma assayed enzymatically, while...

The objective of this study was to use the metabolomic approach investigate early potential biomarkers for lung cancer. Golden Syrian hamsters were randomized into 2 groups. Lung cancer induced in treatment group by a chemical carcinogen, N‐nitrosobis‐2‐(oxopropyl) amine (BOP). Proton nuclear magnetic resonance ( 1 H NMR) spectra, acquired using 500 MHz magnet processed ACD labs software. processed, digitized NMR spectral data analyzed multivariate analysis software, SIMCA P+. Principal...

Prognosis of lung cancer, the leading cause cancer mortality in US improves if detected at an early stage. Curcumin, a dietary phytochemical has proven anti‐inflammatory, antioxidant, and anti‐carcinogenic activity several types. This study aims to evaluate effect curcumin induced hamster model, using metabolomics approach. 32 male Golden Syrian hamsters were randomized into treatment (n=20; by injecting N ‐nitrosobis‐2‐(oxopropyl) amine (BOP);12 weeks), control (n=12) groups. Each group was...

Oxidative stress (OS) is associated with many chronic diseases including cancer, CVD and the normal process of aging. Curcumin, a dietary phytochemical has proven antioxidant, anti‐inflammatory anti‐carcinogenic activity. This study aims to utilize metabolomics evaluate signature oxidative in BOP ( N ‐nitrosobis‐2‐(oxopropyl) amine) treated hamsters. Further, effect curcumin on change metabolomic signatures was investigated. 32 male Golden Syrian hamsters were randomized into treatment...

Curcumin, the polyphenolic pigment from rhizome Curcuma longa, is known for its antioxidant, anti‐inflammatory, antibacterial, and recently, anticarcinogenic properties. The aim of this study was to investigate efficacy curcumin in lung cancer induced hamster model. 32 male Golden Syrian hamsters were divided into test (TO, T2 n= 10 each) control (CO, C2 n=6 groups. Test groups received BOP (N‐nitrosobis (2‐oxopropyl) amine; 10mg/kg), a carcinogen, 12 weeks, while animals saline injections....

Increased plasma LDLc and low HDLc are associated with risk of cardiovascular disease (CVD), the leading cause death worldwide. This study investigated effect an algae infusion, ProAlgaZyme(P) its sub‐fractions on lipids in hamster model. Sixty (n=10/group) male Golden Syrian hamsters (8 w old) fed a high fat diet (30% calories from fat) were randomized into 6 groups: control, P subfractions P1, P2, P3, P4. The infusion (20% v/v) was added to drinking water all experimental groups, while...

Disrupted cholesterol homeostasis in the brain and peripheral circulation is associated with etiology of Alzheimer's disease (AD). Since brain's metabolism segregated, attenuated AD unlikely to be a direct consequence elevated plasma cholesterol. Thus, we hypothesized that there should signals derived from secondary chronic hypercholesterolemia (HC) compromises normal metabolism. We induced HC condition young, middle-aged, older male Brown Norway rats by feeding cholesterol+cholic acid...