Garcinol, a polyisoprenylated benzophenone, is extracted from the rind of fruit Garcinia indica , plant found extensively in tropical regions. Although has been consumed traditionally over centuries, its biological activities, specifically anticancer potential result recent scientific investigations. The anticarcinogenic properties garcinol appear to be moderated via antioxidative, anti-inflammatory, antiangiogenic, and proapoptotic activities. In addition, displays effective epigenetic...

Lung cancer is the leading cause of death among all cancers. Non-small cell lung accounts for 80% with a 5-year survival rate 16%. Notch pathway, especially Notch-1 up-regulated in subgroup non-small patients. Since signaling plays an important role proliferation, differentiation, and apoptosis, down-regulation may exert anti-tumor effects. The objective this study was to investigate whether delta-tocotrienol, naturally occurring isoform Vitamin E, inhibits growth via signaling. Treatment...

MicroRNAs (miRNAs) are small noncoding RNAs that play critical roles in regulating various cellular functions by transcriptional silencing. miRNAs can function as either oncogenes or tumor suppressors (oncomirs), depending on cancer types. In our study, using miRNA microarray, we observed downregulation of the Notch-1 pathway, delta-tocotrienol, correlated with upregulation miR-34a, nonsmall cell lung cells (NSCLC). Moreover, re-expression miR-34a transfection NSCLC resulted inhibition...

Pancreatic cancer (PaCa) is a major health concern due to its aggressiveness and early metastasis. Current treatments for PaCa are limited by development of resistance against therapy. As an alternative strategy, we assessed the combinatorial effect dietary compounds, garcinol curcumin, on human cells (BxPC-3 Panc-1). A significant (<svg style="vertical-align:-0.30096pt;width:56.150002px;" id="M1" height="11.25" version="1.1" viewBox="0 0 56.150002 11.25" width="56.150002"...

Garcinol, or polyisoprenylated benzophenone, isolated from the rind of fruiting bodies Garcinia indica, has been used in traditional medicine for its potential antiinflammatory and antioxidant properties. The objective this study was to investigate effect garcinol on pancreatic cancer (PaCa) cell viability proliferation. For this, 2 human PaCa lines, BxPC-3 Panc-1, with wild mutant k-ras, respectively, were treated (0–40 μM). Garcinol significantly (P < 0.05) inhibited growth (trypan blue...

Delta-tocotrienol (δT), an isomer of vitamin E, exhibits anticancer properties in different cancer types including non-small-cell lung (NSCLC). Yet, anti-invasive effects δT and its underlying cellular mechanism NSCLC have not been fully explored. Matrix metalloproteinase 9 (MMP-9)-based cell migration invasion are critical mechanisms development. The current evidence indicates that MMP-9 is upregulated most patients, the inhibition MMPs involved decreasing metastasis NSCLC. Therefore,...

The growth and development of non-small cell lung cancer (NSCLC) primarily depends on glutamine. Both glutamine essential amino acids (EAAs) have been reported to upregulate mTOR in NSCLC, which is a bioenergetics sensor involved the regulation growth, survival, protein synthesis. Seen as novel concepts development, ASCT2 LAT transporters allow EAAs enter proliferating tumors well send regulatory signal mTOR. Blocking or downregulating these order inhibit uptake would be an excellent...

The triple-negative breast cancer (TNBC) subtype, regardless of their BRCA1 status, has the poorest outcome compared with other subtypes, and currently there are no approved targeted therapies for TNBC. We have previously demonstrated importance RAD6-mediated translesion synthesis pathway in TNBC development/progression chemoresistance, potential therapeutic benefit targeting RAD6 a RAD6-selective small-molecule inhibitor, SMI#9. To overcome SMI#9 solubility limitations, we recently...

Abstract Nuclear factor‐κB (NF‐κB) is a ubiquitous redox‐sensitive transcription factor involved in the pro‐inflammatory response to several factors, including cytokines and oxidative stress. Upon activation, NF‐κB translocates into nucleus binds specific nucleotide sequences. The cellular responses inflammatory stress signals have been implicated disease conditions, such as atherosclerosis, cancer, diabetes, Alzheimer's disease. conventional method for detection of ‐DNA binding activity...

Pancreatic cancer (PC) patients have poor prognosis and survival rate. Gemcitabine, the drug of choice has a dismal 15% response Earlier, we reported that Garcinol alone in combination with gemcitabine showed dose-dependent favorable on PC cell lines. This study probes vivo effects dietary progression transgenic mice (KPC; K-ras p53 conditional mutant). KPC male were divided into: KC- Control diet; KGr-0.05% KGm-Gemcitabine injected; KGG – diet + Gemcitabine injected groups. Changes tumor...

Leptin, a protein hormone secreted by adipose tissue, plays an important role in regulating energy metabolism and the immune response. Despite similar extremes of adiposity, mutant mouse models, db/db, carrying spontaneous deletion active form leptin receptor (LEPR-B) intracellular signaling domain, s/s, specific point mutation leading to dysfunctional LEPR-B-STAT3 pathway, have been shown robust differences glucose homeostasis. This suggests effects leptin, mediated non-STAT3 LEPR-B...

Abstract: Lung cancer is one of the leading causes deaths. Non-small cell lung (NSCLC), with a 5-year survival rate 5% at stage IIIB, accounts for 80%–85% all cancers. Aberrant Notch-1 expressions have been reported in patients and could potentially be beneficial molecular/therapeutic target against NSCLC. Tocotrienols, isomers vitamin E, shown to exhibit antitumor activity via inhibition different signaling pathways tumor cells. Previously, we that delta-tocotrienol downregulates NF-κB....

Non‐small cell lung cancer (NSCLC), accounting for 87% of all cancers with a 5‐year survival rate 16%, is the leading cause global deaths. Tocotrienols, isomers Vitamin E have been shown to exhibit anti‐tumor activity via inhibition different signaling pathways in tumors. The Notch ‐1 pathway has reported be up regulated patients vivo and vitro studies. Therefore, objective this study was investigate interactions effects commercially available tocorienol on Notch‐1 adenocarcinoma (A549)...

Delta-tocotrienol (δT) has been shown to exhibit anti-tumor activity via inhibition of different signaling pathways in tumors including NSCLC. Previously we showed that δT reduced cell invasion the Notch-1 and NF-κb pathway. Matrix metallopeptidase 9 (MMP9) dependent migration are key processes cancer metastasis. Hence its suppression is a promising strategy for therapeutics. The objective this study was investigate possibility MMP9 as underlying mechanism behind anti-metastatic effects on...

Lung cancer, with the majority of cases being non‐small cell lung cancer (NSCLC), is one leading causes death among cancers. NSCLC has exhibited high rate glutamine dependency during its growth and development. It also been shown that uptake plays a mandatory role in essential amino acid activation mTOR kinase. SLC1A5 SLC7A5, transporters, exhibit an important development by transporting into proliferating tumors. Therefore, SLC7A5 are key drug targets potential pharmacological importance....

P-glycoprotein (P-gp) and multidrug resistance-associated protein (MRP) are members of the superfamily ATP-binding cassette transporter proteins. Because ATP-dependent export system has been implicated in release leukotriene C4 (LTC4), we examined roles P-gp MRP LTC4 from normal murine mast cells (MC-9). We have previously shown that MC-9 express at level mRNA. In present study, expression was by anti-MRP Ab, using flow cytometry mRNA PCR Northern blot analyses. were stimulated with calcium...

Oat bran protein isolate was fractionated into Osborne fractions: albumin, globulin, prolamin, and glutelin. Globulin glutelin were the major fractions. The oat globulin (OBG) fraction purified by ammonium sulfate [(NH4)2SO4, 30−80% saturation] precipitation. Purification achieved at 50% saturation of (NH4)2SO4. Purified analyzed gel filtration, sodium dodecyl sulfate−polyacrylamide electrophoresis (SDS−PAGE), circular dichroism (CD) to determine molecular size, subunit weights, secondary...