- dental development and anomalies
- Dental Trauma and Treatments
- Oral and Maxillofacial Pathology
- Endodontics and Root Canal Treatments
- Dental Radiography and Imaging
- Periodontal Regeneration and Treatments
- Dental materials and restorations
- Mesenchymal stem cell research
- Bone and Dental Protein Studies
- Engineering Applied Research
- Oral and Craniofacial Lesions
- Dental Health and Care Utilization
- Force Microscopy Techniques and Applications
- Cleft Lip and Palate Research
- Dental Implant Techniques and Outcomes
- Oral and gingival health research
- Orthodontics and Dentofacial Orthopedics
- Nuclear Engineering Thermal-Hydraulics
- Bone Tissue Engineering Materials
- Dental Erosion and Treatment
- Tumors and Oncological Cases
- Fluid Dynamics and Turbulent Flows
- Oral microbiology and periodontitis research
- Urological Disorders and Treatments
- Lattice Boltzmann Simulation Studies
Yonsei University
2013-2022
Yonsei University College of Dentistry
2008-2022
Nanyang Technological University
2014-2022
Wonkwang University
2020
MTS Systems (United States)
2020
Yonsei University Dental Hospital
2018-2020
Pusan National University
2007-2017
Hillingdon Hospital
2017
Severance Hospital
2007-2015
Yonsei University Health System
2014
Cell fixation is an essential step to preserve cell samples for a wide range of biological assays involving histochemical and cytochemical analysis. Paraformaldehyde (PFA) has been widely used as cross-linking agent. It empirically recognized in gold standard protocol that the PFA concentration fixation, C PFA, 4%. However, it still not quantitatively clear how conventional optimized. Here, we investigated mechanical properties function by using atomic force microscopy scanning ion...
As a simple and efficient technique, the solvent-assisted lipid bilayer (SALB) formation method offers versatile approach to fabricating planar on solid supports. Corresponding mechanistic aspects role of various governing parameters remain, however, be better understood. Herein, we first scrutinized effect concentration (0.01 5 mg/mL) solvent type (isopropanol, n-propanol, or ethanol) SALB silicon oxide in order identify optimal conditions for this process. The obtained fluid-phase layers...
This paper describes the application of a solvent-exchange method to prepare supported membranes containing high fractions cholesterol (up ∼57 mol %) in an apparent equilibrium. The exploits phenomenon reverse-phase evaporation, which deposition lipids alcohol (e.g., isopropanol) is followed by slow removal organic solvent from water-alcohol mixture. turn induces series lyotropic phase transitions successively producing inverse-micelles, monomers, micelles, and vesicles equilibrium with...
Objective The purposes of this study were to isolate and characterize stem cells from inflamed pulp tissue human functional deciduous teeth ( iSHFD ) evaluate the influence fibroblastic growth factor‐2 (FGF‐2) on regenerative potential. Materials methods We successfully isolated mesenchymal (MSCs) dental demonstrated that their potential could be enhanced by application FGF‐2 (20 ng ml −1 during ex vivo expansion. Isolated expanded in characterized using a colony‐forming assay,...
Abstract Since the invention of atomic force microscope (AFM) three decades ago, there have been numerous advances in its measurement capabilities. Curiously, throughout these developments, fundamental nature force-sensing probe—the key actuating element—has remained largely unchanged. It is produced by long-established microfabrication etching strategies and typically composed silicon-based materials. Here, we report a new class photopolymerizable hydrogel nano-probes that are bottom-up...
The accurate measurement of nanoscale mechanical characteristics is crucial in the emerging field soft condensed matter for industrial applications. An atomic force microscope (AFM) can be used to conduct evaluation Young’s modulus on target surface based site-specific spectroscopy. However, there still a lack well-organized study about nanomechanical interpretation model dependence along with cantilever stiffness and radius tip apex materials. Here, we present fast sample’s entire scan...
Hydroxyapatite is a biologically compatible material and major component of dental enamel bone tissue. Because its biocompatibility structural similarity to human teeth the skeletal system, number studies have evaluated application as substitute or restorative material. This study was evaluate differences in bonding strength resistance demineralization between micro-hydroxyapatite nano-hydroxyapatite added self-cured resin-reinforced/modified glass ionomer cement. RelyX used base cement for...
In many studies, adult stem cells have been found in human periodontal ligament (PDL), but most cases they were the permanent teeth. The aim of present study was to characterize from PDL deciduous teeth (dPDLSCs) and compare them with those (pPDLSCs). Stem cell markers examined by a flow cytometric analysis. results vitro differentiation into adipogenic osteogenic lineages analyzed histochemical staining quantitative reverse transcription-polymerase chain reaction (RT-PCR). vivo...
Tunable nanoplasmonic biosensing for lipid and protein applications is reported based on controlling membrane architecture surfaces. The interaction of a peptide with membranes highly sensitive to the top plasmonic nanodisks, measurement response varies in manner which consistent surrounding environment.
Poly(l-lysine) (PLL) adsorption onto various materials has been widely applied as a surface modification strategy and layer-by-layer fabrication method. Considering the role of electrostatic charges, detailed understanding influence solution pH on PLL process is important for optimization coating protocols. Herein, different polar hydrophilic substrates—silica, an amine-terminated self-assembled monolayer (SAM) gold, carboxyl-terminated SAM gold—across range conditions was investigated using...
Ultraviolet (UV) exposure triggers the abnormal production of reactive oxygen (ROS) species and expression matrix metalloproteinases (MMPs) that are responsible for photoaging. Probiotics widely used in healthcare immune enhancement. One probiotic, Lactobacillus buchneri is found Kimchi. This study was aimed at assessing anti-photoaging effect plant extracts fermented with L. (PELB) to develop functional cosmetics. We investigated PELB a UVB-induced photoaging vitro model selected effective...
Objectives The aim of this study was to determine the clinical efficacy newly developed Ortho MTA and Retro , compared conventionally used ProRoot for pulpotomy in primary teeth. Material Methods In randomized trial, 151 molars from 102 children, who met inclusion criteria were 3–10 years old, enrolled. Ultimately, 143 teeth divided a randomized, single‐blind manner into three groups according planned treatment: ( n = 49 teeth), 47 teeth) or teeth). Clinical radiographic follow‐up...
This study focuses on gene expression patterns and functions in human umbilical cord (UC) dental pulp (DP) containing mesenchymal stem cells (MSCs). DP tissues were collected from 25 permanent premolars. UC tissue samples obtained three newborns. Comparative profiles using cDNA microarray analysis the of tooth development-associated MSC-related genes was assessed by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Genes related to cell proliferation,...
The human dental follicle partially differentiates into the periodontal ligament (PDL), but their biological functions are different. gene-expression profiles of and PDL were compared using cDNA microarray technique. Microarray analysis identified 490 genes with a twofold or greater difference in expression, 365 125 which more abundant PDL, respectively. most strongly expressed those related to bone development remodeling (EGFL6, MMP8, FRZB, NELL1), apoptosis chemotaxis (Nox4, CXCL13, CCL2),...