A5041629951- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Peroxisome Proliferator-Activated Receptors
- RNA Interference and Gene Delivery
- Plant Reproductive Biology
- Genetics and Neurodevelopmental Disorders
- Neurobiology and Insect Physiology Research
- Hippo pathway signaling and YAP/TAZ
- Developmental Biology and Gene Regulation
- RNA regulation and disease
- Genomics and Chromatin Dynamics
Institute of Gene Biology
2017-2023
encodes one of the two fly CPEB proteins. These widely conserved proteins bind to 3'UTRs target messenger RNAs (mRNAs) and activate or repress their translation. We show here that a positive autoregulatory loop driven by
Activation of local translation in neurites response to stimulation is an important step the formation long-term memory (LTM). CPEB proteins are a family factors involved LTM formation. The Drosophila protein Orb2 plays role development and function nervous system. Mutations coding region orb2 gene have previously been shown impair We found that deletion 3’UTR similarly results loss Drosophila. As result deletion, content remained same neuron soma, but significantly decreased synapses. Using...
orb is a founding member of the CPEB family translational regulators and required at multiple steps during Drosophila oogenesis. Previous studies showed that mid-oogenesis for translation posterior/germline determinant oskar mRNA dorsal-ventral gurken mRNA. Here, we report also functions upstream these axes determinants in polarization microtubule network (MT). Prior to activation, oocyte MT repolarized. The organizing center posterior disassembled, new established anterior. Repolarization...
ABSTRACT CPEB proteins are conserved translation regulators involved in multiple biological processes. One of these Drosophila, Orb2, is a principal player spermatogenesis. It required for meiosis and spermatid differentiation. During the later process, orb2 mRNA protein localized within developing spermatid. To evaluate role 3′UTR spermatogenesis, we used CRISPR/Cas9 system to generate deletion 3′UTR, orb2R. This disrupts process differentiation but has no apparent effect on meiosis....
Intracellular trafficking plays a critical role in the functioning of highly polarized cells, such as neurons. Transport mRNAs, proteins, and other molecules to synaptic terminals maintains contact between neurons ensures transmission nerve impulses. Cytoplasmic polyadenylation element binding (CPEB) proteins play an essential long-term memory (LTM) formation by regulating local translation synapses. Here, we show that 3'UTR Drosophila CPEB gene orb2 is required for targeting mRNA protein...
Abstract CPEB proteins are conserved translation regulators involved in multiple biological processes. One of these Drosophila , Orb2, is a principal player spermatogenesis. It required for meiosis and spermatid differentiation. During the later process orb2 mRNAs localized within developing spermatid. To evaluate role mRNA 3’UTR spermatogenesis, we used CRISPR/Cas9 system to generate deletion 3’UTR, R . This disrupts differentiation, but has no apparent effect on meiosis. While this appears...