A5003124115- RNA Research and Splicing
- Nuclear Structure and Function
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- RNA regulation and disease
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- interferon and immune responses
- Plant Molecular Biology Research
- Heat shock proteins research
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- Invertebrate Immune Response Mechanisms
- Neurobiology and Insect Physiology Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Endoplasmic Reticulum Stress and Disease
- Cancer-related gene regulation
- Insect Resistance and Genetics
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Genetic Neurodegenerative Diseases
- Signaling Pathways in Disease
- Neuroblastoma Research and Treatments
Russian Academy of Sciences
2005-2024
Engelhardt Institute of Molecular Biology
2013-2024
Institute of Gene Biology
2008-2021
Institute of Molecular Genetics
2015
Metazoan E(y)2/ENY2 is a multifunctional protein important for transcription activation and mRNA export, being component of SAGA/TFTC the export complex AMEX. Here, we show that ENY2 in Drosophila also stably associated with THO, involved mRNP biogenesis. The ENY2–THO required normal development, functioning independently on SAGA THO arrive transcribed region hsp70 gene after its activation, plays an role recruitment. no direct association elongating RNA polymerase II. Recruitment occurs by...
Despite increasing data on the properties of replication origins, molecular mechanisms underlying origin recognition complex (ORC) positioning in genome are still poorly understood. The Su(Hw) protein accounts for activity best-studied Drosophila insulators. Here, we show that recruits histone acetyltransferase SAGA and chromatin remodeler Brahma to Su(Hw)-dependent insulators, which gives rise regions with low nucleosome density creates conditions ORC binding. Depletion leads a dramatic...
The origin recognition complex (ORC) of eukaryotes associates with the replication origins and initiates pre-replication assembly. In literature, there are several reports interaction ORC different RNAs. Here, we demonstrate for first time a direct THSC/TREX-2 mRNA nuclear export complex. was purified from Drosophila embryonic extract found to bind fraction ORC. This occurred via subunits essential viability. Also, associated mRNP, which facilitated by TREX-2. interacted Nxf1 receptor...
AbstractThe Drosophila TATA box-binding protein (TBP)-related factor 2 (TRF2 or TLF) was shown to control a subset of genes different from that controlled by TBP. Here, we have investigated the structure and functions trf2 gene. We demonstrate it encodes two isoforms: previously described 75-kDa TRF2 newly identified 175-kDa version in which same sequence is preceded long N-terminal domain with coiled-coil motifs. Chromatography embryo extracts revealed part multiprotein complex also...
SAGA/TFTC is a histone acetyltransferase complex that has second enzymatic activity because of the presence deubiquitination module (DUBm). Drosophila DUBm consists Sgf11, ENY2 and Nonstop proteins. We show Sgf11 other DUBm-independent functions. It associates with Cbp80 component cap-binding thereby recruited onto growing messenger ribonucleic acid (mRNA); it also interacts AMEX mRNA export essential for hsp70 export, as well general from nucleus. Thus, functions both SAGA biogenesis machinery.
The germline-specific role of telomeres consists chromosome end elongation and proper segregation during early developmental stages. Despite the crucial in germ cells, little is known about telomere biology germline. We analyzed homeostasis Drosophila female germline embryos. A novel function deadenylase complex Ccr4-Not telomeric transcript surveillance mechanism reported. Depletion components causes strong derepression retroelement HeT-A accompanied by poly(A) tail. Dysfunction...
ENY2/Sus1, a protein involved in the coupling of transcription with mRNA export, is component SAGA/TFTC and TREX-2/AMEX complexes. Recently, we have described association ENY2 third complex, THO. Moreover, our data indicate that also associated other factors, both nucleus cytoplasm. Thus, being shared components several complexes, appears to function as an adapter molecule integration cellular processes, particular, subsequent stages gene expression.
The different steps of gene expression are intimately linked to coordinate and regulate this complex process. During transcription, numerous RNA-binding proteins already loaded onto the nascent mRNA package into a messenger ribonucleoprotein particle (mRNP). These often also involved in other than packaging. For example, TREX functions mRNP packaging nuclear export. Previously, we showed that Prp19 splicing (Prp19C) is needed for efficient transcription as well occupancy at transcribed...
Abstract The development of drug resistance reduces the efficacy cancer therapy. Tumor cells can acquire to MDM2 inhibitors, which are currently under clinical evaluation. We generated RG7388-resistant neuroblastoma cells, became more proliferative and metabolically active were less sensitive DNA-damaging agents in vitro vivo, compared with wild-type cells. was associated a mutation p53 protein (His193Arg). This abated its transcriptional activity via destabilization tetrameric p53-DNA...
The TREX-2 complex is essential for the general nuclear mRNA export in eukaryotes. interacts with pore and transcriptional apparatus links transcription to export. However, it remains poorly understood how TREX-2-dependent connected subsequent stages of trafficking. Here, we show that PCID2 subunit Drosophila present cytoplasm cell. cytoplasmic directly NudC protein this interaction maintains its stability cytoplasm. Moreover, associated microtubules. knockdown blocks also affects transport...
The general snRNA gene transcription apparatus has been extensively studied. However, the role of coactivators in this process is far from being clearly understood. Here, we have demonstrated that Drosophila SAGA complex interacts with PBP complex, key component apparatus, and present at promoter regions genes transcribed by both RNA polymerase II III (U6 snRNA). We show Brf1 factor, which a part promoters number Pol III-transcribed genes. Mutations inactivating several subunit resulted...
Abstract Following the transcription step, newly synthesized mRNA is exported from nucleus to cytoplasm and further translation site. The TREX-2 complex involved in step of export cytoplasm. This Drosophila melanogaster consists four proteins: Xmas-2, PCID2, ENY2, Sem1p. In our work, we have shown that deletion C-terminal sequence PCID2 leads a decrease interaction protein with RNA impaired D. .
The Drosophila melanogaster Maleless (MLE) protein is a conserved helicase involved in wide range of gene expression regulation processes. A MLE ortholog, named DHX9, was found many higher eukaryotes, including humans. DHX9 diverse processes, such as genome stability maintenance, replication, transcription, splicing, editing and transport cellular viral RNAs, translation regulation. Some these functions are understood detail today, while most them remain uncharacterized. Study the ortholog...
Abstract In previous studies, we found that the zinc finger proteins Su(Hw) and CG9890 interact with Drosophila SAGA complex participate in formation of active chromatin structure transcription regulation. this research, discovered interaction DUB module another protein, CG9609. ChIP-Seq analysis was performed, CG9609 binding sites genome were identified. Analysis showed they are localized predominantly at gene promoters. The protein has been shown to be involved regulation expression.