A5043153261- Marine Biology and Environmental Chemistry
- Developmental Biology and Gene Regulation
- Echinoderm biology and ecology
- Marine and coastal plant biology
- RNA Research and Splicing
- Genetics, Aging, and Longevity in Model Organisms
- Animal Genetics and Reproduction
- Reproductive Biology and Fertility
- Molecular Biology Techniques and Applications
- Parasite Biology and Host Interactions
- Marine Ecology and Invasive Species
- Chromosomal and Genetic Variations
- Invertebrate Immune Response Mechanisms
- Nematode management and characterization studies
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Sperm and Testicular Function
- Transgenic Plants and Applications
- Marine Bivalve and Aquaculture Studies
- Marine Invertebrate Physiology and Ecology
- Marine Sponges and Natural Products
- Marine Toxins and Detection Methods
- Insect Resistance and Genetics
- Aquatic Invertebrate Ecology and Behavior
- Nicotinic Acetylcholine Receptors Study
- Pluripotent Stem Cells Research
A.V. Zhirmunsky National Scientific Center of Marine Biology Far Eastern Branch of the Russian Academy of Sciences
2013-2023
Institute of Chemistry, Far Eastern Branch of the Russian Academy of Sciences
2023
Institute of Gene Biology
2016-2021
Far Eastern Branch of the Russian Academy of Sciences
2016
Russian Academy of Sciences
2010
Far Eastern Federal University
2003
Agrobacterium-mediated transformation of higher plants is a well-known and powerful tool for transgene delivery to plant cells. In the present work, we studied whether Agrobacterium can transfer genetic information animal (sea urchin) embryos. Sea urchin embryos were co-cultivated with A. tumefaciens strains carrying binary vectors containing nptII marker gene agrobacterial rolC rolB oncogenes. Bacterial plasmid T-DNA-sea DNA junction sites identified in genome these embryos, thus indicating...
Animal germ cells have specific organelles that are similar to ribonucleoprotein complex, called plasm, which is accumulated in eggs. Germ plasm essential for inherited mechanism of line segregation early embryogenesis. Sea urchins Nevertheless, organization plasm-related and their molecular composition still unclear. Another issue whether maternally exists the sea urchin eggs.I analyzed intracellular localization during oogenesis Strongylocentrotus intermedius by using morphological...
orb is a founding member of the CPEB family translational regulators and required at multiple steps during Drosophila oogenesis. Previous studies showed that mid-oogenesis for translation posterior/germline determinant oskar mRNA dorsal-ventral gurken mRNA. Here, we report also functions upstream these axes determinants in polarization microtubule network (MT). Prior to activation, oocyte MT repolarized. The organizing center posterior disassembled, new established anterior. Repolarization...
Nemertea is a phylum of marine worms whose members bear various toxins, including tetrodotoxin (TTX) and its analogues. Despite the more than 30 years studying TTXs in nemerteans, many questions regarding their functions mechanisms ensuring accumulation usage remain unclear. In nemertean Kulikovia alborostrata, we studied TTX 5,6,11-trideoxyTTX concentrations body extracts released mucus, as well aspects TTX-positive-cell excretion system voltage-gated sodium (Nav1) channel subtype 1...
ABSTRACT CPEB proteins are conserved translation regulators involved in multiple biological processes. One of these Drosophila, Orb2, is a principal player spermatogenesis. It required for meiosis and spermatid differentiation. During the later process, orb2 mRNA protein localized within developing spermatid. To evaluate role 3′UTR spermatogenesis, we used CRISPR/Cas9 system to generate deletion 3′UTR, orb2R. This disrupts process differentiation but has no apparent effect on meiosis....
Summary Germ plasm, a cytoplasmic factor of germline cell differentiation, is suggested to be perspective tool for in vitro meiotic differentiation. To discriminate between the: (1) germ plasm-related structures (GPRS) involved meiosis triggering; and (2) GPRS the plasm storage phase, we investigated gametogenesis marine medaka Oryzias melastigma . The mitosis-to-meiosis period are similar males females. In both sexes, five events typically occur: turning primary Vasa-positive granules into...
The sea urchin egg cortex is a peripheral region of eggs comprising cell membrane and adjacent cytoplasm, which contains actin tubulin cytoskeleton, cortical granules some proteins required for early development. Method isolation cortices from embryos was developed in 1970s. Since then, this method has been reliable tool to study protein localization cytoskeletal organization unfertilized during first cleavages. This aimed estimate the reliability RT-qPCR analyze levels maternal transcripts...
Unfertilized eggs contain maternal mRNAs and proteins, which required for maintenance of metabolism regulation development during first stages embryogenesis. are transcriptionally translationally quiescent. After fertilization, activated translation is one major forces that direct early embryogenesis before activation zygotic genome. Here, I discuss data obtained on sea urchins, directly or indirectly indicate can occur in the unfertilized urchin eggs. speculate may be necessary to maintain...
Abstract The sea urchin egg cortex is a peripheral region of eggs consisting cell membrane and adjacent cytoplasm, which contains actin tubulin cytoskeleton, cortical granules some proteins required for early development. Method isolation cortices from embryos has been developed in 70s 20th Century. Since that time this method reliable tool to study protein localization cytoskeletal organization unfertilized during first cleavages. This an estimation reliability RT-qPCR analyze levels...
Abstract Major sperm proteins (MSP) are a nematode-specific system of motor required for amoeboid movement. A number MSP genes vary in different nematode species, but encoded protein sequences reveal high homology between these proteins. In fact, all studies MSPs localization and functions based exclusively on the representatives order Rhabditida belonging to class Chromadorea, while MSP-driven movement Enoplea, another major clade phylum Nematoda is still unconfirmed. this study, we found...