A5036014654- Metabolomics and Mass Spectrometry Studies
- Click Chemistry and Applications
- Chemical Synthesis and Analysis
- Traditional Chinese Medicine Analysis
- Genetics and Neurodevelopmental Disorders
- Autism Spectrum Disorder Research
- Genomics and Chromatin Dynamics
- Traditional Chinese Medicine Studies
- GABA and Rice Research
- Epigenetics and DNA Methylation
- Protein Degradation and Inhibitors
- Photochromic and Fluorescence Chemistry
- Cancer-related gene regulation
- Natural product bioactivities and synthesis
- Plant nutrient uptake and metabolism
- Advanced biosensing and bioanalysis techniques
- Legume Nitrogen Fixing Symbiosis
- Plant biochemistry and biosynthesis
- Catalytic Processes in Materials Science
- Plant Molecular Biology Research
- Microbial Metabolic Engineering and Bioproduction
- Soybean genetics and cultivation
- Acupuncture Treatment Research Studies
- Nuclear Receptors and Signaling
- Bacterial Genetics and Biotechnology
Center for Excellence in Molecular Plant Sciences
2013-2024
Chinese Academy of Sciences
2013-2024
University of Science and Technology of China
2023-2024
Changchun Institute of Applied Chemistry
2023-2024
South China Agricultural University
2022
National Center for Gene Research
2021
Sichuan University
2019-2021
Shanghai Institutes for Biological Sciences
2013-2020
Chengdu University
2019-2020
Harbin Medical University
2010-2013
Abstract Hydrocracking catalysis is a key route to plastic waste upgrading, but the acid site‐driven C−C cleavage step relatively sluggish in conventional bifunctional catalysts, dramatically effecting overall efficiency. We demonstrate here facile and efficient way boost reactivity of sites by introducing Ce promoters into Pt/HY thus achieving better metal‐acid balance. Remarkably, 100 % low‐density polyethylene (LDPE) can be converted with 80.9 selectivity liquid fuels over obtained...
Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao (A. mongolicus, family Leguminosae) is one of the most important traditional Chinese herbs. Among many secondary metabolites it produces, effective bioactive constituents include isoflavonoids and triterpene saponins. The genomic resources regarding biosynthesis these in A. are limited. Although roots primary material harvested for medical use, compounds its regulation not well understood. Therefore, a global transcriptome analysis on...
Seed development is an important process of reproductive and consists embryo endosperm development; both comprise several key processes. To determine investigate the functions dynamic DNA methylome during seed development, we profiled methylation genome wide in a series developmental stages rice (Oryza sativa) by methylcytosine immunoprecipitation followed Illumina sequencing. The results showed that hypermethylated predominantly around non-transposable element (TE) genes, short DNA-TEs,...
Abstract Traditional Chinese medicines (TCMs) have important therapeutic value in long-term clinical practice. However, because TCMs contain diverse ingredients and complex effects on the human body, molecular mechanisms of are poorly understood. In this work, we determined gene expression profiles cells response to TCM components investigate activities at cellular levels. MCF7 were separately treated with 102 different molecules from TCMs, their compared Connectivity Map (CMAP). To...
Abstract The reverse water‐gas shift reaction (RWGS) has been regarded as a promising approach for fighting climate change caused by the excessive emission of greenhouse gas CO 2 , but it still suffers from relatively poor low‐temperature reactivity. Herein, high‐performance RWGS catalyst composed ultrasmall Pt clusters (1.38 nm on average) anchored La O 3 support (Pt NC /LOC), which possesses record production rate 2678 mol −1 h with nearly 100% selectivity at 300 °C, is reported. specific...
We constructed a library of diarylsydnone (DASyd) candidates in search photoclickable reaction toward alkynes, enabling an ultra-accelerated reactivity, while suppressing the background cycloaddition dark. The vitro and vivo protein labelling experiments revealed that photo-accelerated DASyd-alkyne exhibits robust selectivity.
Abstract By exploiting the scope of dipolarophiles, we discovered that endo ‐bicyclo[6.1.0]non‐4‐yn‐9‐ylmethanol (BCN) is an appropriate chemical reporter for 2,5‐diaryltetrazole (DATet) based photoclick reaction, which proceeds via ultrafast cycloaddition rate (∼10 5 M −1 s ). Introduction a 2,6‐(CF 3 ) 2 phenyl moiety on C ‐position tetrazole core brings significant improvement selectivity toward BCN without loss reactivity. The high benefits from combination steric and electrostatic...
Ultra-fast and selective covalent-bond forming reactions with spatiotemporal controllability are foundational for developing a bioorthogonal approach high manipulability. However, it is challenging to exploit reporter functional group achieve these requirements simultaneously. Here, 11H-Dibenzo[c,f][1,2]diazepine set of heterocyclic analogues investigated both their photo-switching natures ability serve as dipolarophiles in photo-click diarylsydnone. Sulfur-containing dibenzothiadiazepine...
Abstract Plants are unique with tremendous chemical diversity and metabolic complexity, which is highlighted by estimates that green plants collectively produce metabolites numbering in the millions. Plant play crucial roles all aspects of plant biology, like growth, development, stress responses, etc. However, lack a reference metabolome for plants, paucity high-quality standard compound spectral libraries related analytical tools, have hindered discovery functional study phytochemicals...
A photo-induced 1,3-dipolar cycloaddition between nitrile imine and highly ring-strained N[double bond, length as m-dash]N double bond a dipolarophile was discovered. The photo-isomerization of carbon-bridged octocyclic azobenzene (CBOA) into its trans-configuration accelerates the ligation reaction at very rapid rate (28 400 M-1 s-1). CBOA-based photo-click proved to be bioorthogonal. In addition, NoxaB peptide successfully cross-linked by CBOA stapler which plays dual role: photo-control...
Abstract Background Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is one of the most common herbs widely used in South and East Asia, to enhance people’s health reinforce vital energy. Despite its prevalence, however, knowledge about phytochemical compositions metabolite biosynthesis very limited. Results An integrated metabolomics transcriptomics analysis using state-of-the-art UPLC-Q-Orbitrap mass spectrometer advanced bioinformatics pipeline were conducted study global...
GOLDEN2-LIKE (GLK) transcription factors are a subfamily of GARP family factors, which play an essential function in plant growth and development as well stress response during abiotic biotic conditions. This study reports GLK genes the Arabidopsis thaliana genome in-depth identified 55 AtGLK genome. Phylogenetic analyses resolved these gene clusters into seven groups. A Ka/Ks ratios analysis indicated that they had experienced purifying selection. Many cis elements present promoter regions...
Fragile X syndrome (FXS) is a prevailing genetic disorder of intellectual disability and autism. There no efficacious medication for FXS. Through in silico screening with public database, computational analysis transcriptome profile FXS mouse neurons predicts therapeutic value an FDA-approved drug trifluoperazine. Systemic administration low-dose trifluoperazine at 0.05 mg/kg attenuates multiple FXS- autism-related behavioral symptoms. Moreover, alteration caused by suggests new mechanism...
Herein, we design and synthesize a series of photoactivatable β-diarylsydnone-l-alanines (DASAs), which have excellent photo-reactivity with high fluorescence turn-on toward alkenes in biocompatible environment. The environmental sensing properties the resulting fluorescent pyrazoline-alanine facilitate its probing capability. By introducing DASA residue on side chain linear peptides, macrocyclic peptides from situ photo-cyclization alkene exhibited fluorogenic translocation through live...
Rice (Oryza sativa) is one of the most important grain crops, which serves as food source for nearly half world population. The study rice development process well related strategies production has made significant progress. However, comprehensive on different tissues at both transcriptomic and metabolic flux level across stages was lacked. In this study, we performed RNA-Seq characterized expression profiles differentiated from Oryza sativa Zhonghua 11, including leaves, sheath, stamen,...
SUMMARY Chemical compositions of crops are great agronomical importance, as serve resources for nutrition, energy, and medicines human livestock. For crop metabolomics research, the lack reference metabolome high‐quality compound mass spectra, well utilities metabolic profiling, has hindered discovery functional study phytochemicals in crops. To meet these challenging needs, we have developed Crop Metabolome database (abbreviated CropMetabolome) that is dedicated to construction metabolome,...
Background Eukaryotic transcription is accompanied by combinatorial chromatin modifications that serve as functional epigenetic markers. Composition of specifies histone codes regulate the associated gene. Discovering novel regulatory relationships are general interest. Methodology/Principal Findings Based on premise interaction hypothesized to influence CpG methylation, we present a closeness measure characterize interactions epigenomic features. The applied genome-wide methylation and...
The GIF gene family is one of the plant transcription factors specific to seed plants. members are expressed in all lateral organs produced by apical and floral meristems contribute development leaves, shoots, flowers, seeds. This study identified eight genes soybean genome clustered them into three groups. Analyses Ka/Ks ratios divergence times indicated that they had undergone purifying selection during species evolution. RNA-sequence relative expression patterns these GmGIF tended be...
Fragile X syndrome (FXS) is caused by mutations in the FMR1 gene. It a form of heritable intellectual disability and autism. Despite recent advance elucidating disease mechanisms, there no efficacious medication. Because de novo drug development lengthy process, repurposing existing FDA-approved drugs offers an opportunity to clinical intervention for FXS. Our previous study with transcriptome analysis predicts potential therapeutic effects vorinostat on
Abstract A set of photo‐switchable monopeptides derived from cis ‐β‐dibenzodiazocine‐ l ‐alanine ( ‐DBDAA) have been designed and synthesized, which are capable photo‐click reacting with diaryltetrazoles or diarylsydnones in a hydrophobic phospholipid bilayer environment. The DBDAA include both tail on C ‐terminal, providing high affinity toward lipid membrane, modularized functional moiety N enabling rapid optimization the self‐assembly strength to form multifunctional supramolecules. With...