A5089200010- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Blood transfusion and management
- Venous Thromboembolism Diagnosis and Management
- Anesthesia and Pain Management
- Enzyme Structure and Function
- Hair Growth and Disorders
- Blood groups and transfusion
- Cancer, Hypoxia, and Metabolism
- melanin and skin pigmentation
- Neonatal Respiratory Health Research
- Alzheimer's disease research and treatments
- Total Knee Arthroplasty Outcomes
- Hops Chemistry and Applications
- vaccines and immunoinformatics approaches
- Pain Management and Opioid Use
- Bacteriophages and microbial interactions
- Epigenetics and DNA Methylation
- Cellular transport and secretion
- Acute Ischemic Stroke Management
- Nausea and vomiting management
- RNA modifications and cancer
- Autophagy in Disease and Therapy
Stony Brook University
2025
Konkuk University
2024
National Health Insurance Service Ilsan Hospital
2024
Stanford University
2022-2023
California Institute of Technology
2015-2021
Cleveland Clinic
2013-2014
The Neurological Institute
2013
Neurology, Inc
2003
The authors tested the primary hypothesis that perioperative IV lidocaine administration during spine surgery (and in postanesthesia care unit for no more than 8 h) decreases pain and/or opioid requirements initial 48 postoperative hours. Secondary outcomes included major complications, nausea and vomiting, duration of hospitalization, quality life.One hundred sixteen adults having complex were randomly assigned to (2 mg·kg·h) or placebo unit. Pain was evaluated with a verbal response scale....
First steps of translocation elucidated Ribosomes synthesizing membrane or secretory proteins are targeted to the endoplasmic reticulum (ER) in eukaryotic cells by signal recognition particle (SRP). Upon reaching ER, SRP interacts with its receptor promote transfer sequence protein-conducting channel translocon. Kobayashi et al. studied ribosomal complex that forms on which and interact newly synthesized protein The observed organization assembly reveals roles multiple eukaryotic-specific...
Abstract Protein biogenesis is essential in all cells and initiates when a nascent polypeptide emerges from the ribosome exit tunnel, where multiple ribosome-associated protein factors (RPBs) direct proteins to distinct fates. How RPBs spatiotemporally coordinate with one another affect accurate an emerging question. Here, we address this question by studying role of cotranslational chaperone, polypeptide-associated complex (NAC), regulating substrate selection signal recognition particle...
Significance Correct protein biogenesis is crucial for all cells. Numerous factors including molecular chaperones, modification enzymes, and protein-targeting machineries bind near the ribosome exit site can access nascent protein. How proteins are accurately selected into correct pathway in such a crowded environment an emerging question central to accurate biogenesis. Using chemical biology biochemical biophysical tools, we show that major cotranslational chaperone, trigger factor,...
<i>Background and Objective:</i> Although percutaneous transluminal angioplasty (PTA) is an effective treatment modality in the coronary peripheral arterial diseases, its efficacy for intracranial atherosclerotic stenosis has not been verified. We assessed long-term outcome of PTA symptomatic middle cerebral artery (MCA) stenosis. <i>Methods:</i> performed 10 patients with high-grade (>70%) on M1 segment MCA, who had either recurrent transient ischemic attacks...
Significance The universally conserved signal recognition particle (SRP) delivers ∼30% of the proteome to appropriate cellular membrane. How SRP achieves efficient and selective protein targeting in eukaryotes remains elusive. Here, we show that a functional sequence on nascent polypeptide confers significant kinetic privilege ribosome-bound during recruitment receptor, thereby enabling rapid membrane SRP-dependent substrates. In addition, single-molecule spectroscopy revealed ribosome-...
Single-molecule and cryo-EM studies reveal molecular acrobatics of signal recognition particle that initiates protein translocation.
Phloroglucinol (PG) is one of the abundant isomeric benzenetriols in brown algae.Due to its polyphenolic structure, PG exhibits various biological activities.However, impact on anagen signaling and oxidative stress human dermal papilla cells (HDPCs) unknown.In this study, we investigated therapeutic potential for improving hair loss.A non-cytotoxic concentration increased anagen-inductive genes transcriptional activities β-Catenin.Since several are regulated by β-Catenin, further experiments...
Volatile anesthetic prices differ substantially. But differences in drug-acquisition cost would be inconsequential if hospitalization were prolonged by more soluble anesthetics. The authors tested the hypothesis that duration of is with isoflurane anesthesia.Initially, queried their electronic records and used propensity matching to generate homogeneous sets adults having inpatient noncardiac surgery who given desflurane, sevoflurane, isoflurane. then conducted a prospective alternating...
Molecular recognition features (MoRFs) provide interaction motifs in intrinsically disordered protein regions to mediate diverse cellular functions. Here we report that a MoRF element, located the linker domain of mammalian signal particle (SRP) receptor and conserved among eukaryotes, plays an essential role sensing ribosome during cotranslational targeting endoplasmic reticulum. Loss SRP (SR) largely abolishes ability activate SRP-SR assembly impairs targeting. These results demonstrate...
Aging and neurodegeneration entail diverse cellular molecular hallmarks. Here, we studied the effects of aging on transcriptome, translatome, multiple layers proteome in brain a short-lived killifish. We reveal that causes widespread reduction proteins enriched basic amino acids is independent mRNA regulation, it not due to impaired proteasome activity. Instead, identify cascade events where aberrant translation pausing leads reduced ribosome availability resulting remodeling independently...
Supplementary Data from Notch Signal Activates Hypoxia Pathway through HES1-Dependent SRC/Signal Transducers and Activators of Transcription 3
Abstract Signal recognition particle (SRP) is a universally conserved targeting machine that couples the synthesis of ~30% proteome to their proper membrane localization 1,2 . In eukaryotic cells, SRP recognizes translating ribosomes bearing hydrophobic signal sequences and, through interaction with receptor (SR), delivers them Sec61p translocase on endoplasmic reticulum (ER) How ensures efficient and productive initiation protein translocation at ER not well understood. Here, single...