A5029629862- Cancer-related Molecular Pathways
- Virus-based gene therapy research
- Cancer Research and Treatments
- RNA Research and Splicing
- Cancer, Hypoxia, and Metabolism
- Neuroblastoma Research and Treatments
- Molecular Biology Techniques and Applications
- Microtubule and mitosis dynamics
- Viral Infectious Diseases and Gene Expression in Insects
- Telomeres, Telomerase, and Senescence
- Ubiquitin and proteasome pathways
- Cellular transport and secretion
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Renal and related cancers
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Phagocytosis and Immune Regulation
- Cancer, Lipids, and Metabolism
- Heat shock proteins research
- Cancer Genomics and Diagnostics
- Viral gastroenteritis research and epidemiology
- Glioma Diagnosis and Treatment
- DNA Repair Mechanisms
University of Otago
2016-2025
Maurice Wilkins Centre
2015-2024
University of Auckland
2011-2024
Children's Medical Research Institute
2013-2023
The University of Sydney
2013-2023
Malaghan Institute of Medical Research
2020-2021
Genesis Laboratories
2011
Wake Forest University
2011
Asbestos Diseases Research Institute
2011
CSL (Australia)
2011
In a prospective-longitudinal study of representative birth cohort, we tested why stressful experiences lead to depression in some people but not others. A functional polymorphism the promoter region serotonin transporter (5-HT T) gene was found moderate influence life events on depression. Individuals with one or two copies short allele 5-HT T exhibited more depressive symptoms, diagnosable depression, and suicidality relation than individuals homozygous for long allele. This...
MYCN gene amplification in neuroblastoma drives a expression program that correlates strongly with aggressive disease. Mechanistically, trimethylation of histone H3 lysine 4 (H3K4) at target promoters is strict prerequisite for this transcriptional to be enacted. WDR5 H3K4 presenter has been found have an essential role trimethylation. For reason, study, we investigated the relationship between WDR5-mediated and N-Myc programs cells. upregulated Gene analysis revealed genes included those...
Mutants dl312, dl314, hr1, and hr3 with mutations in region E1A of adenovirus type 5 were defective for the induction cell cycle abnormalities detectable by flow cytometry, DNA replication, thymidine kinase production, chromosome aberrations did not synthesize viral DNA-binding protein (E2A) rat cells. dl311, a leaky mutant, induced effects at high multiplicity only one three experiments, synthesized protein. hr7 (E1B) gave wild-type response all tests. dl313 was also positive tests,...
Y-box binding factor 1 (YB-1) has been associated with prognosis in many tumor types. Reduced YB-1 expression inhibits cell growth, but the mechanism is unclear. mRNA levels were compared grade and histology using microarray data from 771 breast cancer patients disease-free survival distant metastasis–free 375 of those who did not receive adjuvant therapy. Microarrays further searched for genes that had correlated mRNA. Small interfering RNA (siRNA) was used to study effects reduced on...
Viscometric titrations of bacteriophage PM2 closed circular DNA, in addition to spectrophotometric and fluorometric methods, were used investigate the mode DNA binding a number antitrypanosomal antitumor compounds. Several classes compounds identified which failed unwind appeared have large site at least 4 base pairs often showed considerable selectivity poly[d(AT)] as opposed poly[d(GC)]. The included antiviral antibiotics distamycin netropsin, bisamidines such trypanocidal drug berenil,...
Abstract ∆122p53 mice (a model of ∆133p53 isoform) are tumour-prone, have extensive inflammation and elevated serum IL-6. To investigate the role IL-6 we crossed with null mice. Here show that loss reduced JAK-STAT signalling, tumour incidence metastasis. We also activates RhoA-ROCK signalling leading to cell invasion, which is IL-6-dependent can be by inhibition pathways. Similarly, Δ133p53 these pathways, resulting in invasive migratory phenotypes colorectal cancer cells. Gene expression...
The p53 tumor suppressor plays a major role in preventing development by transactivating genes to remove or repair potentially tumorigenic cells. Here we show that the Y-box-binding protein, YB1, acts as negative regulator of p53. Using reporter assays YB1 represses transcription promoter sequence-specific manner. We also reduces endogenous levels p53, which turn activity. Conversely, inhibiting variety cell lines induces activity, resulting significant apoptosis via p53-dependent pathway....
Abstract The endocytic protein dynamin II (dynII) participates in cell cycle progression and has roles centrosome cohesion cytokinesis. We have described a series of small-molecule inhibitors [myristyl trimethyl ammonium bromides (MiTMAB)] that competitively interfere with the ability to bind phospholipids prevent receptor-mediated endocytosis. now report dynII functions specifically during abscission phase cytokinesis MiTMABs exclusively block this step cycle. Cells treated (MiTMAB...
The retinoblastoma protein (RB)–E2F1 pathway has a central role in regulating the cell cycle. Several PAX proteins (tissue-specific developmental regulators), including PAX8, interact with RB protein, and thus regulate cycle directly or indirectly. Here, we report that PAX8 expression is frequent renal carcinoma, bladder, ovarian thyroid cancer lines, silencing of lines leads to striking reduction E2F1 its target genes, as well proteasome-dependent destabilization RB1 mRNA level remaining...
The p53 protein is a pivotal tumor suppressor that frequently mutated in many human cancers, although precisely how prevents tumors still unclear. To add to its complexity, several isoforms of have now been reported. Δ133p53 isoform generated from an alternative transcription initiation site intron 4 the gene (Tp53) and lacks N-terminus. Elevated levels observed variety tumors. explore functions Δ133p53, we created mouse expressing N-terminal deletion mutant (Δ122p53) corresponds Δ133p53....
<title>Abstract</title> Mutations in the tumor suppressor, <italic>TP53</italic>, are prevalent human cancers; yet their functional implications remain unclear. Through comprehensive pan-cancer database analysis, we identified H179 mutations <italic>TP53</italic> as significantly associated with poor disease-free survival across multiple cancer types. Functional studies lung, ovarian, and prostate cells overexpressing p53<sup>H179R/Y</sup> mutants revealed that these not only defective...
Abstract The molecular basis for alternative lengthening of telomeres (ALT), a prognostic marker glioma patients, remains unknown. We examined TP53 status in relation to telomere maintenance mechanism (TMM) 108 patients with glioblastoma multiforme and two anaplastic astrocytoma from New Zealand United Kingdom. Tumor samples were analyzed respect telomerase activity, length, ALT-associated promyelocytic leukemia nuclear bodies determine their TMM. mutation was by direct sequencing coding...