A5027413583- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Neurogenesis and neuroplasticity mechanisms
- Cell Adhesion Molecules Research
- Optical Imaging and Spectroscopy Techniques
- Optical Coherence Tomography Applications
- Inflammatory mediators and NSAID effects
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
- Chemokine receptors and signaling
- Hepatitis B Virus Studies
- Peptidase Inhibition and Analysis
- Neuroendocrine regulation and behavior
- Cardiac Imaging and Diagnostics
- Cardiovascular Effects of Exercise
- Monoclonal and Polyclonal Antibodies Research
- Prenatal Screening and Diagnostics
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cardiomyopathy and Myosin Studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Fetal and Pediatric Neurological Disorders
- Graphene and Nanomaterials Applications
- Liver physiology and pathology
- Collagen: Extraction and Characterization
- Liver Disease Diagnosis and Treatment
Hospital General Universitario Gregorio Marañón
2016-2025
Universidad Carlos III de Madrid
2015-2024
Centro de Investigación Biomédica en Red de Salud Mental
2017-2024
Instituto de Salud Carlos III
2021
Comunidad de Madrid
2021
The Francis Crick Institute
2010-2012
Universidad Autónoma de Madrid
2000-2007
Hospital Universitario de La Princesa
2004
Hospital Universitario de Canarias
2000
Universidad de La Laguna
2000
Neural stem cells (NSCs) reside in specialized niches the adult mammalian brain, including subventricular zone and dentate gyrus, which act to control NSC behavior. Among other cell types within these niches, NSCs are found close proximity blood vessels. We carried out an analysis of interaction between endothelial NSCs, show that betacellulin (BTC), a member EGF family one several signaling molecules made by former, induces proliferation prevents spontaneous differentiation culture. When...
Hepatocellular carcinoma is strongly associated with chronic infection by the hepatitis B virus (HBV) and has poor prognosis due to intrahepatic metastasis. HBx often only HBV protein detected in hepatic tumor cells; however, its contribution invasion metastasis not been established so far. In this work, we show that enhances cell invasion, both vivo vitro. The increased invasive capacity induced mediated an upregulation of membrane-type 1 matrix metalloproteinase (MT1-MMP) expression, which...
Abstract Objective To explore the potential involvement of chemokine system in synoviocyte‐mediated tissue destruction rheumatoid arthritis (RA), we studied expression profile receptors and their function migration, proliferation, matrix metalloproteinase (MMP) production cultured fibroblast‐like synoviocytes (FLS) from RA patients. Methods The presence CC CXC on FLS was at messenger RNA (mRNA) level by reverse transcriptase–polymerase chain reaction cell surface flow cytometry. Variations...
Hepatocellular carcinoma is strongly associated with chronic infection by the hepatitis B virus (HBV) and has poor prognosis due to intrahepatic metastasis. HBx often only HBV protein detected in hepatic tumor cells; however, its contribution invasion metastasis not been established so far. In this work, we show that enhances cell invasion, both vivo vitro. The increased invasive capacity induced mediated an upregulation of membrane-type 1 matrix metalloproteinase (MT1-MMP) expression, which...
Abstract A disintegrin and metalloproteinase domain (ADAM) proteins are a family of transmembrane glycoproteins with heterogeneous expression profiles proteolytic, cell-adhesion, -fusion, -signaling properties. One its members, ADAM-8, is expressed by several cell types including neurons, osteoclasts, leukocytes and, although it has been implicated in osteoclastogenesis neurodegenerative processes, little known about role immune cells. In this study, we show that ADAM-8 constitutively...
// Michele Cioffi 1 , Sara M. Trabulo 1, 2 Mireia Vallespinos Deepak Raj Tony Bou Kheir Meng-Lay Lin Julfa Begum Ann-Marie Baker 3 Ala Amgheib Jaimy Saif 4 Manuel Perez 5 Joaquim Soriano Desco 6, 7, 8 Maria Victoria Gomez-Gaviro Lorena Cusso Diego Megias Alexandra Aicher Christopher Heeschen Stem Cells & Cancer Group, Spanish National Research Centre (CNIO), Madrid, Spain in Ageing, Barts Institute, Queen Mary University of London, UK for Tumour Biology, School Clinical Sciences,...
Arrhythmogenic cardiomyopathy/arrhythmogenic right ventricular cardiomyopathy (ARVC) is an inherited cardiac disease characterized by fibrofatty replacement of the myocardium, resulting in heart failure and sudden death. The most aggressive arrhythmogenic cardiomyopathy/ARVC subtype ARVC type 5 (ARVC5), caused a p.S358L mutation TMEM43 (transmembrane protein 43). function localization are unknown, as mechanism which causes disease. Here, we report characterization first transgenic mouse...
Optical tissue clearing techniques can render large-scale samples and whole organs transparent to apply cell-resolution 3D imaging techniques, revolutionizing the fields of biological research pathological diagnosis, providing never-before seen structural information. There are several widely used protocols, but they have shortcomings, both in complexity use suboptimal results certain tissues organs. Here, we describe a new method dubbed "White Clear" (WaC) using 2,2'-thiodiethanol (TDE) as...
Iron-based nanoparticles have emerged as promising candidates for diverse biomedical applications, including cell separation, targeted drug delivery, hyperthermia therapy, and magnetic resonance imaging. This study reports the scalable synthesis of high-magnetization iron-based with controlled anisotropic shapes, achieved via a two-step process. Hematite nanoparticles, featuring nanocube, nanoellipse, nanoneedle morphologies, were synthesized through hydrolysis ferric chloride in presence...
BACKGROUND: Arrhythmogenic right ventricular cardiomyopathy type 5 (ARVC5) is the most aggressive of ARVC, caused by a fully penetrant missense mutation (p.S358L) in TMEM43 (transmembrane protein 43). Pathologically, disease characterized dilation cardiac chambers and fibrofatty replacement myocardium, which results heart failure sudden death. Current therapeutic options are limited, no specific therapies targeting primary cause have been proposed. METHODS: We investigated whether...
Animal behavioral tests are essential to understand the bases of neurologic and psychological disorders, which can be evaluated by different methodological experimental models. However, quantification results is limited considerable amount time needed for manual evaluation high costs automated analysis software. To overcome these limitations, we describe here a new, open source toolbox ImageJ, called Mouse Behavioral Analysis Toolbox (MouBeAT), designed analyze in rodents semi-automatically....
The CUBIC tissue clearing protocol has been optimized to produce translucent immunostained whole chicken embryos and embryo brains. When combined with multispectral light sheet microscopy, the validated presented here provides a rapid, inexpensive reliable method for acquiring accurate histological images that preserve three-dimensional structural relationships single-cell-level resolution in early-stage embryos, brains of late-stage embryos.
The ability to acquire high resolution 3D images of the heart enables study diseases more in detail. In this work, CUBIC (clear, unobstructed brain imaging cocktails and computational analysis) clearing protocol was optimized for thick mouse sections enhance penetration depth confocal microscope lasers into tissue. addition, enhances antibody tissue by a factor five. present deep high-quality image acquisition allowing much accurate assessment cellular structural changes that underlie diseases.
It has been recently described that some non-steroidal anti-inflammatory drugs (NSAIDs) are able to induce the shedding of L-selectin in neutrophils, an adhesion molecule plays essential role inflammatory response. We have found that, according this capability, NSAIDs could be grouped into three categories. A high releaser group (flufenamic, meclofenamic, and mefenamic acids, diclofenac aceclofenac), a moderate releasers (aspirin, indomethacin, nimesulide, flurbiprofen, ketoprofen),...
Abstract Selective Plane Illumination Microscopy (SPIM) has become an emerging technology since its first application for 3D in-vivo imaging of the development a living organism. An extensive number works have been published, improving both speed acquisition and resolution systems. Furthermore, multispectral allows effective separation overlapping signals associated with different fluorophores from spectrum over whole field-of-view analyzed sample. To eliminate need using fluorescent dyes,...