A5062590670- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Ion channel regulation and function
- Cardiovascular Effects of Exercise
- Nitric Oxide and Endothelin Effects
- Viral Infections and Immunology Research
- Protein Kinase Regulation and GTPase Signaling
- Cellular Mechanics and Interactions
- Cardiac Fibrosis and Remodeling
- Cardiac electrophysiology and arrhythmias
- Muscle activation and electromyography studies
- Cardiovascular Function and Risk Factors
- Neuroscience and Neuropharmacology Research
- Adipose Tissue and Metabolism
- Cell Adhesion Molecules Research
- Muscle metabolism and nutrition
- Stress Responses and Cortisol
- Pain Mechanisms and Treatments
- Chronic Lymphocytic Leukemia Research
- Renin-Angiotensin System Studies
- Force Microscopy Techniques and Applications
- Genetic Neurodegenerative Diseases
- Metabolism, Diabetes, and Cancer
- Nicotinic Acetylcholine Receptors Study
- Biotin and Related Studies
University of Cologne
2015-2024
Medizinische Hochschule Brandenburg Theodor Fontane
2024
University Hospital Cologne
2011-2022
Matrix Research (United States)
2019
Munich Cluster for Systems Neurology
2015-2017
Ludwig-Maximilians-Universität München
2015-2017
German Centre for Cardiovascular Research
2017
University of Dundee
2015
Heidelberg University
1990-2011
Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases
2011
Cardiac troponins in blood are the most preferred markers of myocardial damage. The fact that they normally not found circulation provides a high level clinical sensitivity and specificity even when cardiac lesions small. After injury, enter circulation, where can be used for diagnosis acute coronary syndromes. Thus, paramount disease classification risk stratification. However, little is known about long-term effects released on function.In this study we prepared recombinant murine troponin...
Induced pluripotent stem (iPS) cells generated from accessible adult of patients with genetic diseases open unprecedented opportunities for exploring the pathophysiology human in vitro. Catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) is an inherited cardiac disorder that caused by mutations ryanodine receptor 2 gene (RYR2) and characterized stress-induced arrhythmia can lead to sudden death young individuals. The aim this study was generate iPS a patient CPVT1 determine...
Significance Myocardial inflammation leads in many cases to cardiomyopathy and contributes progressive heart failure. The exact pathological mechanism of disease induction progression the setting failure is unknown. High-mobility group box 1 (HMGB1), an evolutionarily abundant highly conserved protein, promotes cardiac inflammation, turn immunity, as a damage-associated molecular pattern. HMGB1 stimulates at least part, through interaction with its principal binding partner RAGE (receptor...
Developmental changes in force-generating capacity and Ca2+ sensitivity of contraction murine hearts were correlated with myosin heavy chain (MHC) troponin (Tn) isoform expression, using Triton-skinned fibres. The maximum Ca2+-activated isometric force normalized to the cross-sectional area (FCSA) increased mainly during embryogenesis continued increase at a slower rate until adulthood. During prenatal development, FCSA about 5-fold from embryonic day (E)10.5 E19.5, while amount MHC total...
Background — Cellular cardiomyoplasty is discussed as an alternative therapeutic approach to heart failure. To date, however, the functional characteristics of transplanted cells, their contribution function, and most importantly, potential benefit this treatment remain unclear. Methods Results Murine ventricular cardiomyocytes (E12.5–E15.5) labeled with enhanced green fluorescent protein (EGFP) were into cryoinjured left walls 2-month-old male mice. Ultrastructural analysis cryoinfarction...
Developmental changes in force-generating capacity and Ca2+ sensitivity of contraction murine hearts were correlated with myosin heavy chain (MHC) troponin (Tn) isoform expression, using Triton-skinned fibres. The maximum Ca2+-activated isometric force normalized to the cross-sectional area (FCSA) increased mainly during embryogenesis continued increase at a slower rate until adulthood. During prenatal development, FCSA about 5-fold from embryonic day (E)10.5 E19.5, while amount MHC total...
Background— Despite the widespread use of cardiac troponins for diagnosis myocyte injury and risk stratification in acute disorders, little is known about long-term effects released on function. Recently, we showed that an autoimmune response to troponin I (cTnI) induces severe inflammation subsequent fibrosis myocardium. This disorder predisposes heart failure death mice. Methods Results— To investigate role cTnI-specific T cells, cells were isolated from splenocytes mice immunized with...
Application of antibodies against cardiac troponin I (cTnI-Ab) can induce dilation and dysfunction the heart in mice. Recently, we demonstrated that immunization with cTnI induces inflammation fibrosis myocardium Others have shown auto-antibodies to are present patients acute coronary syndrome, but little is known about clinical relevance detected cTnI-Ab.First, anti-cTnI anti-cTnT antibody titres were measured sera from 272 dilated- (DCM) 185 ischaemic- (ICM) cardiomyopathy. Secondly, 108...
1. The aim of this study was to determine whether the low molecular mass GTPase RhoA or related proteins are involved in carbachol‐ and high‐K(+)‐induced contractions intact intestinal smooth muscle as well carbachol‐induced increase Ca2+ sensitivity myofilaments permeabilized preparations. 2. force production beta‐escin‐permeabilized enhanced preparations that were loaded with constitutively active mutant RhoA, Val14RhoA, inhibited by exoenzyme C3 from Clostridium botulinum, which...
We examined length changes of individual half-sarcomeres during and after stretch in actively contracting, single rabbit psoas myofibrils containing 10-30 sarcomeres. The were fluorescently immunostained so that both Z-lines M-bands sarcomeres could be monitored by video microscopy simultaneously with the force measurement. Half-sarcomere lengths determined processing images tracking fluorescent Z-line M-band signals. Upon Ca2+ activation, rise force, active predominantly shorten but to...
The α2β1 integrin is a collagen-binding protein with very high affinity for collagen I. It also binds several other collagens and laminins it expressed by many cells, including keratinocytes fibroblasts in the skin. In past, was suggested to be responsible cell attachment, spreading migration on monomeric I contraction of three-dimensional lattices. view these functions, normal development fertility α2-deficient mice, which we generated targeting α2 gene, came as surprise. This existence...
The protective effects of 5′-AMP–activated protein kinase (AMPK) on the metabolic syndrome may include direct resistance artery vasomotor function. However, precise actions AMPK microvessels and their potential interaction are largely unknown. Thus, we set to determine activation vascular smooth muscle tone underlying mechanisms. Resistance arteries isolated from hamster mouse exhibited a pronounced endothelium-independent dilation pharmacological by 2 structurally unrelated compounds (PT1...
Chemically skinned (Lubrol WX) cardiac muscle fibres produce half‐maximum isometric tension at pCa 6.18 (pH 6.7) in presence of MgATP (10 mM). After addition cGMP (5 μM) and cGMP‐depenent protein kinase (0.1 μM), the required for activation is 5.96, while maximum not affected. Similar shifts tension/pCa‐relationship have been observed after incubation with cAMP catalytic subunit cAMP‐dependent kinase. The shift Ca 2+ ‐sensitivity associated an increased incorporation radioactivity into a M r...
Collagen XVIII is an important component of the extracellular matrix and expressed in basement membranes. Its degradation results generation endostatin claimed to possess antiangiogenic activity. To date, only limited knowledge exists with regard cellular signaling this molecule. We show single-cell measurements using Ca 2+ indicator fura-2 acetoxy methylester (fura-2 AM) nitric oxide (NO) 4,5-diaminofluorescein diacetate that application (ES) (5 pmol/L, 100 ng/mL) induced spikes increase NO...
Rationale: This study was performed to gain insights into novel therapeutic approaches for the treatment of autoimmune myocarditis. Objective: Chemical stimulation efferent arm vagus nerve through activation nicotinic acetylcholine receptor subtype-7α (α7-nAChR) has been shown be protective in several models inflammatory diseases. In present study, we investigated potentially effect on Methods and Results: A/J mice were immunized with cardiac troponin I (TnI) induce Mice exposed drinking...