A5068435270- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Cardiac electrophysiology and arrhythmias
- Nicotinic Acetylcholine Receptors Study
- Pain Mechanisms and Treatments
- Adenosine and Purinergic Signaling
- Pharmacological Effects and Toxicity Studies
- Cholinesterase and Neurodegenerative Diseases
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Heme Oxygenase-1 and Carbon Monoxide
- Alzheimer's disease research and treatments
- Trace Elements in Health
- Neuroinflammation and Neurodegeneration Mechanisms
- Cerebral Venous Sinus Thrombosis
- Nitric Oxide and Endothelin Effects
- Neuroscience of respiration and sleep
- Nuclear Receptors and Signaling
- Botulinum Toxin and Related Neurological Disorders
- Cerebrovascular and Carotid Artery Diseases
- Analytical Chemistry and Sensors
- Peroxisome Proliferator-Activated Receptors
- Neurosurgical Procedures and Complications
- Fatty Acid Research and Health
- Cardiac Arrest and Resuscitation
University of Plymouth
2018
Medical University of South Carolina
2009-2013
Clemson University
2010-2013
Universidad Autónoma de Madrid
2006-2011
University of South Carolina
2011
National Institute on Aging
2009-2010
National Institutes of Health
2009-2010
National Institute of Mental Health
2010
Institute on Aging
2009
Universidade Federal de Santa Catarina
2003-2009
Induced pluripotent stem (iPS) cells generated from accessible adult of patients with genetic diseases open unprecedented opportunities for exploring the pathophysiology human in vitro. Catecholaminergic polymorphic ventricular tachycardia type 1 (CPVT1) is an inherited cardiac disorder that caused by mutations ryanodine receptor 2 gene (RYR2) and characterized stress-induced arrhythmia can lead to sudden death young individuals. The aim this study was generate iPS a patient CPVT1 determine...
We have previously shown that an acute administration of adenosine produces antidepressant-like effect in the forced swimming test (FST) and tail suspension mice. In this work we investigated contribution nitric oxide (NO)-cyclic guanosine monophosphate (cGMP) pathway to adenosine's FST since signalling is assumed play important role depression. The (10 mg/kg i.p.) was prevented by pre-treatment with l-arginine (750 i.p.), S-nitroso-N-acetyl-penicillamine (SNAP, 25 µg/site i.c.v), or...
Ca(2+)-independent phospholipase A(2)β (iPLA(2)β) selectively hydrolyzes docosahexaenoic acid (DHA, 22:6n-3) in vitro from phospholipid. Mutations the PLA2G6 gene encoding this enzyme occur patients with idiopathic neurodegeneration plus brain iron accumulation and dystonia-parkinsonism without accumulation, whereas mice lacking show neurological dysfunction neuropathology after 13 months. We hypothesized that DHA metabolism signaling would be reduced 4-month-old iPLA(2)β-deficient overt...
Abstract Activation of neuronal nicotinic acetylcholine receptors (nAChR) provides neuroprotection against different toxic stimuli that often lead to overproduction reactive oxygen species (ROS) and cell death. ROS production has been related with disease progression in several neurodegenerative pathologies such as Alzheimer’s or Parkinson’s diseases. In this context, we investigated here if the exposure bovine chromaffin cells potent nAChR agonist epibatidine protected rotenone (30 μmol/L)...
This study investigated the effect of haeme oxygenase-1 (HO-1) in nociception induced by formalin injection mice hind paw. Intraperitoneal (i.p.) administration cobalt protoporphyrin (CoPP, an HO-1 inducer, 5mg/kg) 24h before test, inhibited nociceptive response during second phase, but not first phase test. The CoPP was prevented treatment with tin (SnPP, inhibitor activity) administered either i.p. (25mg/kg, 30 min test) or intraplantar (400 nmol/paw, 5 routes. Human embryonic kidney (HEK)...
In vitro studies show that docosahexaenoic acid (DHA) can be released from membrane phospholipid by Ca(2+)-independent phospholipase A(2) (iPLA(2)), plasmalogen PLA(2) or secretory PLA(2 (sPLA2)), but not Ca(2+)-dependent cytosolic (cPLA2), which selectively releases arachidonic (AA). Since glutamatergic NMDA (N-methyl-D-aspartate) receptor activation allows extracellular Ca(2+) into cells, we hypothesized brain DHA signaling would altered in rats given NMDA, to the extent vivo was mediated...
Epibatidine has shown antinociceptive effects in various pain models, being 200-fold more potent than morphine. Previous results from our laboratory demonstrated that HO-1 overexpression an effect the formalin test. Furthermore, epibatidine was able to induce haeme oxygenase-1 (HO-1). So, aim of this study investigate induced by nociception elicited injection mice hindpaw. Administration (4 microg/kg) 24h before test reduced nociceptive response during first phase and second This prevented...
Abstract Early white matter (WM) changes are common in dementia and may contribute to functional decline. We here examine this phenomenon an induced model for the first time. report a novel selective form of myelin injury as manifestation tauopathy adult central nervous system. Myelin pathology rapidly followed induction P301 tau mutation associated with fronto‐temporal humans (rTG4510 line). Damage involved focal disruption ad‐axonal lamella internal oligodendrocyte tongue process, by...
Acute and chronic hypoxias are common cardiac diseases that lead often to arrhythmia impaired contractility. At the cellular level it is unclear whether suppression of Ca(2+) channels (Ca(V)1.2) results directly from oxygen deprivation on channel protein or mediated by intermediary proteins affecting channel. To address this question we measured early effects hypoxia (5-60 s, P(O(2)) < 5 mmHg) current (I(Ca)) tested involvement kinase A (PKA) phosphorylation, Ca(2+)/calmodulin-mediated...