A5049602345- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- PI3K/AKT/mTOR signaling in cancer
- Genetic factors in colorectal cancer
- Cancer Immunotherapy and Biomarkers
- Lung Cancer Research Studies
- Pancreatic and Hepatic Oncology Research
- Genomics and Rare Diseases
- Metastasis and carcinoma case studies
- Inflammatory mediators and NSAID effects
- Hypothalamic control of reproductive hormones
- Chronic Lymphocytic Leukemia Research
- Radiomics and Machine Learning in Medical Imaging
- Angiogenesis and VEGF in Cancer
- Pulmonary Hypertension Research and Treatments
- Cancer Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Pain Mechanisms and Treatments
- Neuroendocrine regulation and behavior
- Health Systems, Economic Evaluations, Quality of Life
- Cancer therapeutics and mechanisms
- Pharmaceutical studies and practices
- Lung Cancer Diagnosis and Treatment
- Melanoma and MAPK Pathways
Austin Health
2006-2024
University of Minnesota
2024
Freeman Hospital
2024
Vanderbilt University Medical Center
2021-2024
Royal Brisbane and Women's Hospital
2023
Vanderbilt University
2022-2023
University of Manchester
2007-2021
Cancer Research UK Manchester Institute
2007-2021
CellCentric (United Kingdom)
2021
The Christie NHS Foundation Trust
2011-2018
Lung cancer is the leading cause of cancer-related death worldwide. Non-small-cell lung (NSCLC) lacks validated biomarkers to predict treatment response. This study investigated whether circulating tumor cells (CTCs) are detectable in patients with NSCLC and what their ability might be provide prognostic information and/or early indication patient response conventional therapy.In this single-center prospective study, blood samples for CTC analysis were obtained from 101 previously untreated,...
Abstract Small cell lung cancer (SCLC) is characterized by prevalent circulating tumour cells (CTCs), early metastasis and poor prognosis. We show that SCLC patients (37/38) have rare CTC subpopulations co-expressing vascular endothelial-cadherin (VE-cadherin) cytokeratins consistent with vasculogenic mimicry (VM), a process whereby form ‘endothelial-like’ vessels. Single-cell genomic analysis reveals characteristic changes in both VE-cadherin-positive -negative CTCs. Higher levels of VM are...
Importance Despite consistent public health recommendations, obesity rates in the US continue to increase. Physical activity recommendations do not account for individual genetic variability, increasing risk of obesity. Objective To use activity, clinical, and data from All Us Research Program (AoURP) explore association higher body mass index (BMI) with level physical needed reduce incident Design, Setting, Participants In this population–based retrospective cohort study, participants were...
There is no consensus on the optimal protocol for isolation of circulating tumor DNA. We report our comparison several extraction methods and variables that may affect yield, quality, contamination DNA was extracted from plasma serum five healthy volunteers by means four different commercially available kits yield quantified real‐time PCR. using optimum kit an additional 10 patients with small cell lung cancer (SCLC) to compare fragment size in versus those SCLC controls. Time sample...
This study investigated the potential clinical utility of circulating free DNA (cfDNA) as a source BRAF mutation detection in patients enrolled into phase II AZD6244, specific MEK1/2 inhibitor, with advanced melanoma. mutations were detected using Amplification Refractory Mutation System allele-specific PCR. status was assessed serum-derived cfDNA from 126 and 94 matched tumour samples. Of samples, 45 (47.9%) found to be positive (BRAF+). Serum-derived BRAF+ 33 (26.2%) including five samples...
We previously demonstrated that circulating tumour cells (CTCs) are detectable by the MelCAM and high molecular weight melanoma-associated antigen (HMW-MAA)-dependent CellSearch platform. However, CTCs which do not express these capture detection markers CellSearch. Consequently, we explored use of isolation size epithelial (ISET), a marker independent, filtration-based device to determine prevalence heterogeneity in metastatic cutaneous melanoma patients. Ninety patients were prospectively...
Mutations in the PIK3CA gene (phosphoinositide-3-kinase, catalytic, alpha polypeptide) have recently been described a number of cancers, and their detection is currently limited because low sensitivity conventional sequencing techniques.We combined Amplification Refractory Mutation System (ARMS; AstraZeneca) allele-specific PCR Scorpions (DxS) to develop assays for tumor-borne mutations used real-time high-throughput multiplexed most commonly reported mutants (H1047L, H1047R, E542K,...