A5010313168- Physiological and biochemical adaptations
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Neurobiology and Insect Physiology Research
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Aquaculture Nutrition and Growth
- Renin-Angiotensin System Studies
- Selenium in Biological Systems
- Regulation of Appetite and Obesity
- Redox biology and oxidative stress
- Sulfur Compounds in Biology
- Metabolism and Genetic Disorders
- High Altitude and Hypoxia
- Analytical Chemistry and Sensors
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Adipokines, Inflammation, and Metabolic Diseases
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Cephalopods and Marine Biology
- Nicotinic Acetylcholine Receptors Study
- Veterinary Pharmacology and Anesthesia
- Pregnancy and preeclampsia studies
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Ovarian function and disorders
University of Calabria
2015-2025
Istituto Nazionale per le Ricerche Cardiovascolari
2017
University of Naples Federico II
1991-2012
Azienda Ospedaliera S.Maria
2012
University of Modena and Reggio Emilia
2012
Magna Graecia University
2011
Inserm
2007
Université de Strasbourg
2007
University of Padua
1987-1991
King's College Hospital
1963-1964
Previous experimental and clinical data suggest impaired decidual trophoblast invasion in patients with polycystic ovarian syndrome (PCOS).The objective of the study was to test hypothesis that endovascular pregnant PCOS is clarify potential mechanisms involved.This an case-control study.The conducted at academic Departments Obstetrics Gynecology Unit Pathology (Italy).Forty-five subjects screened from a wide population women waiting for legal pregnancy termination were included final...
Abstract Aim Selenoprotein T (SelT or SELENOT ) is a novel thioredoxin‐like enzyme whose genetic ablation in mice results early embryonic lethality. SelT exerts an essential cytoprotective action during development and after injury through its redox‐active catalytic site. This study aimed to determine the expression regulation of mammalian heart normal pathological conditions evaluate cardioprotective effect SelT‐derived peptide, SelT43‐52( PSELT encompassing redox motif which key function,...
Inspired by the recent advancements in understanding binding mode of sulfonylurea-based NLRP3 inhibitors to sensor protein, we developed new replacing central sulfonylurea moiety with different heterocycles. Computational studies evidenced that some designed compounds were able maintain important interaction within NACHT domain target protein similarly most active inhibitors. Among studied compounds, 1,3,4-oxadiazol-2-one derivative 5 (INF200) showed promising results being prevent...
Cardiac lipotoxicity is an important contributor to cardiovascular complications during obesity. Given the fundamental role of endoplasmic reticulum (ER)-resident Selenoprotein T (SELENOT) for cardiomyocyte differentiation and protection regulation glucose metabolism, we took advantage a small peptide (PSELT), derived from SELENOT redox-active motif, uncover mechanisms through which PSELT could protect cardiomyocytes against lipotoxicity. To this aim, modeled cardiac by exposing H9c2...
Under low O2, the heart of Carassius auratus (goldfish) shows an enhanced hemodynamics. This is observed in ex vivo cardiac preparations from animals acclimated to both normoxia and short-term (4 days) moderate hypoxia perfused for 90 min with a hypoxic medium. hypoxia, this associated higher ventricular muscularity expanded mitochondrial compartment. To date, little known about putative influence on contribution energy metabolism. Similarly, it remains unexplored whether exposure...
The catecholamine release-inhibitory catestatin [Cts; human chromogranin (Cg) A 352-372 , bovine CgA 344-364 ] is a vasoreactive and anti-hypertensive peptide derived from CgA. Using the isolated avascular frog heart as bioassay, in which interactions between endocardial endothelium subjacent myocardium can be studied without confounding effects of vascular endothelium, we tested direct cardiotropic Cts its interaction with β-adrenergic (isoproterenol, ISO) endothelin-1 (ET-1) signaling....
Abstract The exceptional hypoxia tolerance of the goldfish heart may be achieved through activation an alternative mechanism recruiting first product anaerobic glycolysis (i.e. piruvate). This hypothesis led to design a classical mass spectrometry based proteomic study identify in cardiac proteins that associated with maintaining function under normoxia and hypoxia. A selective protein solubilization, SDS PAGE, trypsin digestion MALDI MS/MS analysis allowed identification 12 most stable...
ABSTRACT Nothing is known about the effects of nitric oxide (NO) on cardiac performance in fish. Using an vitro working heart preparation that generates physiological values output pressure, and ventricular work power, we assessed NO eel Anguilla anguilla. We examined basal (at constant preload, afterload rate), cholinergic stimulation Frank–Starling response (preload-induced increases at rate). The synthase (NOS) inhibitors NG -monomethyl-L-arginine (L-NMMA) L-N5 (1-iminoethyl)ornithine...
This study was designed to evaluate the effect of severe peripheral arterial insufficiency on carnitine concentrations and acetyltransferase palmitoyltransferase activities in ischemic skeletal muscles patients with vascular disease.Nine biopsy specimens were obtained from five undergoing reconstructive surgery. Biopsies 35 normal subjects served as controls. Ischemic showed a significant reduction total control value 20.9 +/- 5.2 11.6 6.2 nmol/mg noncollagen protein (p less than 0.01). A...
Abstract The goldfish ( Carassius auratus ) is known for its physiologic ability to survive even long periods of oxygen limitation (hypoxia), adapting the cardiac performance requirements peripheral tissue perfusion. We here investigated effects short-term moderate hypoxia on heart, focusing ventricular adaptation, in terms hemodynamics and structural traits. Functional evaluations revealed that animals exposed 4 days environmental increased hemodynamic evaluated ex vivo preparations. This...
Abstract Maladaptive cardiac hypertrophy contributes to the development of heart failure (HF). The oxidoreductase Selenoprotein T (SELENOT) emerged as a key regulator during rat cardiogenesis and acute protection. However, its action in chronic settings dysfunction is not understood. Here, we investigated role SELENOT pathophysiology HF: (i) by designing small peptide (PSELT), recapitulating activity via redox site, assessed beneficial preclinical model HF [aged spontaneously hypertensive...
Hydrogen sulfide (H₂S) has recently emerged as an important mediator of mammalian cardiovascular homeostasis. In nonmammalian vertebrates, little is known about the cardiac effects H₂S. This study aimed to evaluate, in avascular heart frog, Rana esculenta, whether and what extent H₂S affects performance, mechanism action responsible for observed effects. Results were analyzed relation those obtained rat heart, used model. Isolated perfused (working Langendorff) hearts, Western blot analysis,...
Evidence from both mammalian and nonmammalian vertebrates indicates that intracardiac nitric oxide (NO) facilitates myocardial relaxation, ventricular diastolic distensibility, and, consequently, the Frank-Starling response, i.e., preload-induced increase of cardiac output. Since nitrite ion (NO(2)(-)), major storage pool bioactive NO, recently emerged as a cardioprotective endogenous modulator, we explored its influence on response in eel, frog, rat hearts, used paradigms fish, amphibians,...
ACh exerted a biphasic effect in the vitro working heart of Rana esculenta. High concentrations (10 −7 M) depressed stroke volume (SV) and work (SW) by ∼30% with shorter systolic phase reduced peak pressure. Doses from 10 −10 M induced positive response peaking at −8 (SV: +8.6%; SW: +6.5%) prolonged without affecting Atropine pirenzepine blocked both negative effects ACh. Pretreatment Triton X-100 (0.1 ml, 0.05%) or nitric oxide (NO)-cGMP pathway antagonists ( N G -nitro-l-arginine,...
ABSTRACT Endocardial endothelial morphology and the physiological modulatory role of nitric oxide (NO) were studied in an vitro preparation working intact heart frog Rana esculenta, which lacks coronary vasculature is thus devoid a vascular endothelium. En face confocal scanning laser microscopy samples perfused fixed hearts demonstrated presence NO synthase as cytoplasmic constituent endocardial cells. Stroke volume (as measure performance paced hearts) stroke work index systolic function)...