A5057591029- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Microscopic Colitis
- Education, Psychology, and Social Research
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Respiratory viral infections research
- Protein purification and stability
- Chemical and Physical Properties of Materials
- Pneumonia and Respiratory Infections
- Insect and Pesticide Research
- Carbon Nanotubes in Composites
- Linguistics, Language Diversity, and Identity
- Nosocomial Infections in ICU
- Nanoparticles: synthesis and applications
- Historical Geopolitical and Social Dynamics
- Infections and bacterial resistance
- Taxation and Legal Issues
- Operations Management Techniques
- Essential Oils and Antimicrobial Activity
- EFL/ESL Teaching and Learning
- Catholicism and Religious Studies
- Antimicrobial Peptides and Activities
- Effects of Environmental Stressors on Livestock
- Gastrointestinal motility and disorders
Charles University
2010-2022
University Hospital in Motol
2015-2022
Czech Academy of Sciences
2005
Mycobacterium tuberculosis, the etiologic agent of is an intracellular pathogen alveolar macrophages. These cells avidly take up nanoparticles, even without use specific targeting ligands, making nanotherapeutics ideal for treatment such infections. Methoxy poly(ethylene oxide)- block-poly(ε-caprolactone) nanoparticles several different polymer blocks' molecular weights and sizes (20-110 nm) were developed critically compared as carriers rifampicin, a cornerstone in tuberculosis therapy. The...
The ethanol extracts of 16 Czech medicinal plants, namely, Allium sativum L. (Alliaceae), Artemisia absinthium (Asteraceae), vulgaris Carum carvi (Apiaceae), Consolida regalis Gray (Ranunculaceae), Cucurbita pepo (Cucurbitaceae), Daucus carota Dryopteris filix-mas (L.) Schott (Dryopteridaceae), Erigeron canadensis Hedera helix (Araliaceae), Inula helenium Juglans regia (Juglandaceae), Satureja hortensis (Lamiaceae), Tanacetum vulgare Thymus and Valeriana officinalis (Valerianaceae), have...
In 2014, 18 hospitals in the Czech Republic participated a survey of incidence Clostridium difficile infections (CDI) country. The mean CDI was 6.1 (standard deviation (SD):7.2) cases per 10,000 patient bed-days and 37.8 (SD: 41.4) admissions. testing frequency 39.5 tests 25.4) 255.8 164.0) A total 774 C. isolates were investigated, which 225 (29%) belonged to PCR ribotype 176, 184 (24%) 001. Multilocus variable-number tandem repeat analysis (MLVA) revealed 27 clonal complexes formed by 84%...
The microwave (mw) plasma torch at atmospheric pressure has been studied for carbon nanotube (CNT) synthesis.The depositions were carried out on silicon substrates with 5-15 nm thin iron catalytic overlayers from the mixture of argon, hydrogen and methane.The optical emission spectroscopy showed presence C 2 CH radicals as well excited atoms.The vicinity substrate influenced relative intensities increased .For fixed mw power, temperature strongly depended its position respect to nozzle...
To characterise and compare twenty-eight Finnish Clostridium difficile RT027-like isolates, selected based on the presence of 18 bp deletion in tcdC gene toxin profile (A, B, binary), with eleven RT027 isolates from different geographical areas time periods.Twenty-eight C. 11 comparative strains were characterised by capillary-electrophoresis (CE) ribotyping, multi-locus variable tandem-repeats analysis (MLVA), sequence typing (MLST), sequencing gyrA fragments. Susceptibility to moxifloxacin...
Clostridium difficile is the causative agent of C. infection (CDI) that could be manifested by diarrhea, pseudomembranous colitis or life-threatening toxic megacolon. The spread certain strains represents a significant economic burden for health-care. epidemic successful are also associated with severe clinical features CDI. Therefore, proteomic study has been conducted comprises proteomes released from in vitro cultured panel eight different PCR ribotypes (RTs) and employs combination...
Clostridium difficile infection (CDI) is a major cause of antibiotic-associated diarrhoea. Given an increasing CDI incidence and global spread epidemic ribotypes, 1-year study was performed to analyse the molecular characteristics C. isolates associated clinical outcomes from patients diagnosed with in Internal Medicine department at University Hospital Motol, Prague February 2013 2014.A total 85 unformed stool samples were analysed laboratory confirmed 30 (6.8 cases per 10,000 patient bed...
Aim: To perform a retrospective analysis of the high occurrence Clostridium difficile infection in surgical department Czech tertiary care hospital and to identify weaknesses C. (CDI) prevention control policies. Methods: Clinical epidemiological data on eleven CDI cases were collected. isolates characterized by capillary electrophoresis ribotyping, multilocus variable tandem repeat (MLVA), gyrA gene fragment sequencing, erm(B) PCR amplification. Antibiotic susceptibility metronidazole,...
Clostridioides (Clostridium) difficile infection (CDI) is the most common causative pathogen of health care-associated gastrointestinal infections; however, due to overlap clinical symptoms with those other causes acute gastroenteritis, selection appropriate laboratory test difficult. From April October 2018, 640 stool samples requested for CDI testing were examined using mariPOC and Gastro (ArcDia), which allows detection C. glutamate dehydrogenase (GDH) toxin A/B, norovirus genogroups GI...
Legionnaires’ disease is a severe form of lung infection caused by bacteria belonging to the genus Legionella. The severity depends on both host immunity and L. pneumophila virulence. objective this study was describe pathological spectrum acute pneumonia virulent clinical isolate serogroup 1, sequence type 62. In A/JOlaHsd mice, we compared two infectious doses, namely, 104 106 CFU, their impact mouse status, bacterial clearance, pathology, blood count parameters studied. Acute resembling...