A5034839806- Metabolomics and Mass Spectrometry Studies
- Birth, Development, and Health
- Pancreatic function and diabetes
- Microbial bioremediation and biosurfactants
- Metabolism, Diabetes, and Cancer
- Diet and metabolism studies
- Diabetes Treatment and Management
- Neonatal and fetal brain pathology
- Photosynthetic Processes and Mechanisms
- Environmental Chemistry and Analysis
- Effects of Radiation Exposure
- Hemoglobin structure and function
- Fermentation and Sensory Analysis
- Nitric Oxide and Endothelin Effects
- Chronic Kidney Disease and Diabetes
- Metabolism and Genetic Disorders
- Reproductive Physiology in Livestock
- Cancer, Lipids, and Metabolism
- Infant Nutrition and Health
- Liver Disease Diagnosis and Treatment
- Lipid metabolism and biosynthesis
- Dialysis and Renal Disease Management
- Diet, Metabolism, and Disease
- Bioinformatics and Genomic Networks
- Phytochemical and Pharmacological Studies
University of Patras
2017-2025
Abstract Introduction The diagnosis of Growth Hormone Deficiency (GHD) during childhood has been the subject much controversy over last few years. Aiming to accurate medical treatment, there is a need for biomarker discovery. Objective To characterize metabolic profile GHD children, examine effect GH administration on signature, and investigate correlations between metabolites IGF-1. Methods Nuclear Magnetic Resonance (NMR)-based untargeted targeted metabolomic approach applied study...
Blood is one of the most used biofluids in metabolomics studies, and serum plasma fractions are routinely as a proxy for blood itself. Here we investigated association networks an array 29 metabolites identified quantified via NMR samples two cohorts ∼1000 healthy donors each. A second study 377 individuals was to extract from same individual on which set 122 were detected using FIA-MS/MS. Four different inference algorithms (ARANCE, CLR, CORR, PCLRC) obtain consensus networks. The obtained...
APOC3 is produced mainly by the liver and intestine approximately half of plasma associates with HDL. Though it was believed that HDL simple binding to preexisting particles, recent data support biogenesis APOC3-containing (APOC3-HDL) requires Abca1. Moreover, APOC3-HDL contributes triglyceride homeostasis preventing association triglyceride-rich lipoproteins. Interestingly, also shows positive correlation morbidly obese phenotype. However, roles in functionality adipose tissue metabolic...
Abstract Context Inhibitors of sodium-glucose cotransporters-2 have cardio- and renoprotective properties. However, the underlying mechanisms remain indeterminate. Objective To evaluate effect dapagliflozin on renal metabolism assessed by urine metabolome analysis in patients with type 2 diabetes. Design Prospective cohort study. Setting Outpatient diabetes clinic a tertiary academic center. Patients Eighty hemoglobin A1c > 7% metformin monotherapy were prospectively enrolled....
Chronic Kidney Disease (CKD) is considered as a major public health problem it can lead to end-stage kidney failure, which requires replacement therapy. A prompt and accurate diagnosis, along with the appropriate treatment, delay CKD's progression, significantly. Herein, we sought determine whether CKD etiology be reflected in urine metabolomics during its early stage. This achieved through analysis of metabolic fingerprint from 108 patients by means Nuclear Magnetic Resonance (NMR)...
Synthetic surfactants are used in several industries, including manufacturing, pharmaceutical and cosmetic’s, food feed, agriculture, petroleum environmental remediation for their ability to adsorb fluid solid-water interfaces. However, widespread use synthetic preparation through environmentally unfavorable processes counterbalances the value of this class reagents. This fact has stimulated new efforts exploit natural sources surfactants, such as classes bacterial systems or manipulation...
Urine metabolomics is gaining traction as a means of identifying metabolic signatures associated with health and disease states. Thirty-one (31) late preterm (LP) neonates admitted to the neonatal intensive care unit (NICU) 23 age-matched healthy LPs maternity ward tertiary hospital were included in study. Proton nuclear magnetic resonance (1H NMR) spectroscopy was employed for urine metabolomic analysis on 1st 3rd days life neonates. The data analyzed using univariate multivariate...
One of the most critical medical conditions occurring after preterm birth is neonatal sepsis, a systemic infection with high rates morbidity and mortality, chiefly amongst neonates hospitalized in Neonatal Intensive Care Units (NICU). sepsis categorized as early-onset (EOS) late-onset (LOS) regarding time disease onset. The accurate early diagnosis or prognosis have hurdles to overcome, since there are not specific clinical signs laboratory tests. Herein, need for biomarkers presents, goals...
Aberrant angiogenesis is a hallmark for cancer and inflammation, key notion in drug repurposing efforts. To delineate the anti-angiogenic properties of amifostine human adult model via 3D cell metabolomics upon stimulant-specific manner, cellular assay that recapitulates physiology action was coupled to untargeted by liquid chromatography–mass spectrometry nuclear magnetic resonance spectroscopy. The early events its most prominent stimulants (vascular endothelial growth factor-A or...
Sea buckthorn berries (Hippophaë rhamnoides L.) (SB) are considered as a fruit with high nutritional value plethora of bioactive ingredients. The present work focusses on the analysis whole NMR metabolic profile SB grown in an organic orchard Meteora/Greece. In parallel, this study validates/highlights qualitative characteristics osmotic processed according to fresh fruit. composition metabolites was elucidated through sophisticated high-resolution spectroscopy. lipophilic maintains...
Premature adrenarche (PA) for long time was considered a benign condition but later has been connected to various diseases in childhood and adulthood which remains controversial.To investigate the effect of premature on metabolic phenotype, correlate clinical biochemical data with profile children PA.Nuclear magnetic resonance (NMR)-based untargeted targeted metabolomic approach combination multivariate univariate statistical analysis applied study profiles PA. Plasma, serum, urine samples...
In this study, non-targeted 1 H NMR fingerprinting was used in combination with multivariate statistical analyses for the classification of Greek currants based on their geographical origins (Aeghion, Nemea, Kalamata, Zante, and Amaliada). As techniques, Principal Component Analysis (PCA) Partial Least Squares Discriminant (PLS-DA) were carried out. To elucidate different components according to PDO (Protected Designation Origin), products from Aeghion (Vostizza) statistically compared each...
The binding of p-coumaroyl glucose to glycogen phosphorylase (GP; a pharmaceutical target for the development antihyperglycaemic drugs) has been studied by kinetics, and X-ray crystallography while its effect HepG2 cells metabolism assessed NMR metabolomics. p-Coumaroyl is potent inhibitor human liver GP with Ki value 213 μΜ that binds at active site enzyme. Comparative structural analysis chemically similar inhibitors reveals basis inhibitory potency. metabolomics revealed in presence...
Abstract Soluble guanylate cyclase (sGC) is considered as the primary NO receptor across several known eukaryotes. The main interest regarding biological role and its function, focuses on H-NOX domain of β1 subunit. This in active form bears a ferrous b type heme prosthetic group, which facilitates binding other diatomic gases. key point that still needs to be answered how protein selectively binds redox state coordination determines upon present genomes both prokaryotes eukaryotes, either...