In comparison to synthetically derived surfactants, biosurfactants produced from microbial culture are generally regarded by industry as being more sustainable and possess lower toxicity. One major class of rhamnolipids primarily Pseudomonas aeruginosa. Due its pathogenicity rhamnolipid synthesis this species is viewed commercially nonviable, such there a significant focus identify alternative producers rhamnolipids.To achieve this, we phenotypically screened marine bacteria for...

This study aimed to identify and characterise biosurfactant compounds produced by bacteria associated with a marine eukaryotic phytoplankton bloom. One strain, designated MCTG214(3b1), was isolated enrichment polycyclic aromatic hydrocarbons based on 16S rDNA, gyrB sequencing found belong the genus Pseudomonas, however not related P. aeruginosa. Cell-free supernatant samples of strain MCTG214(3b1) at stationary phase showed significant reductions in surface tension. HPLC-MS NMR analysis...

Chronic Kidney Disease (CKD) is considered as a major public health problem it can lead to end-stage kidney failure, which requires replacement therapy. A prompt and accurate diagnosis, along with the appropriate treatment, delay CKD's progression, significantly. Herein, we sought determine whether CKD etiology be reflected in urine metabolomics during its early stage. This achieved through analysis of metabolic fingerprint from 108 patients by means Nuclear Magnetic Resonance (NMR)...

Pleiotrophin (PTN) is a heparin-binding growth factor that plays significant role in tumor and angiogenesis. We have previously shown order for PTN to induce migration of endothelial cells, binding both α(ν) β(3) integrin its receptor protein tyrosine phosphatase beta/zeta (RPTPβ/ζ) required. In the present study we show synthetic peptide corresponding last 25 amino acids C-terminal region (PTN(112-136) ) inhibited angiogenesis vivo chicken embryo chorioallantoic membrane (CAM) assay...

ABSTRACT Amyloid deposits to the islets of Langerhans are responsible for gradual loss pancreatic β‐cells leading type II diabetes mellitus. Human mature islet amyloid polypeptide (hIAPP), a 37‐residue hormone, has been identified as primary component fibrils forming these deposits. Several individual segments along entire sequence length hIAPP have nominated regions with increased amyloidogenic potential, such 8–20, 20–29, and 30–37. A smaller fragment 8–20 region, spanning residues 8–16...

Biosurfactants produced from microbial sources are increasingly viewed by industry as more sustainable and less toxic alternatives to their chemically derived counterparts. One major class of biosurfactant that has the potential for commercial exploitation rhamnolipids. Rhamnolipids composed one or two rhamnose monosaccharides covalently bonded fatty acid chains varying molecular weights. The producer rhamnolipid is Pseudomonas aeruginosa, however this a known human pathogen many industries...

The heat stability of egg white stabilised emulsions was studied in the presence monoglycerides, lecithin and rhamnolipid biosurfactants. Rhamnolipids have a greater effect on reduction emulsion droplet particle size than either or with both latter having virtually no even at high concentration. all surfactants is concentration dependent. There trend towards minimum (represented by first order rate constant, k) for molar ratio (R) protein to surfactant R = 50 34 61 rhamnolipids. However,...

Synthetic surfactants are used in several industries, including manufacturing, pharmaceutical and cosmetic’s, food feed, agriculture, petroleum environmental remediation for their ability to adsorb fluid solid-water interfaces. However, widespread use synthetic preparation through environmentally unfavorable processes counterbalances the value of this class reagents. This fact has stimulated new efforts exploit natural sources surfactants, such as classes bacterial systems or manipulation...

Analogs of GnRH, including [DLeu6, desGly1o]-GnRH-NHEt (leuprolide, commercial product), have been widely used in oncology to induce reversible chemical castration. Several studies provided evidence that, besides their pituitary effects, GnRH analogs may exert direct antiproliferative effects on tumor cells. To study the effect modifications positions 4 and 6 leuprolide prostate cancer cell proliferation, we synthesized 12 new analogs. All lacked carboxy-terminal Gly10-amide an ethylamide...

Abstract The self‐assembling capacity of certain molecules can be exploited for a diverse range biomedical applications. ionic complementary peptide RADA‐16 is well‐known its propensity to self‐assemble, which derives from architectural arrangement. Herein, we describe rational synthetic strategies synthesis based on fragment condensation, and subsequent purification through optimized methods. Our methodology should prove suitable the preparation other peptides prone self‐assembly.

Abstract Lamprey gonadotropin‐releasing hormone type III (lGnRH‐III) is an isoform of GnRH isolated from the sea lamprey (Petromyzon marinus) with negligible endocrine activity in mammalian systems. Data concerning superior direct anticancer lGnRH‐III have been published, raising questions on structure–activity relationship. We synthesized 21 analogs rational amino acid substitutions and studied their effect PC3 LNCaP prostate cancer cell proliferation. Our results question importance acidic...

Soluble guanylate cyclase (sGC) is the main receptor of nitric oxide (NO) and by converting GTP to cGMP regulates numerous biological processes. The β 1 subunit most abundant, α heterodimer, harbors an N-terminal domain called H-NOX, responsible for heme NO binding thus sGC activation. Dysfunction NO/sGC/cGMP axis causally associated with pathological states such as heart failure pulmonary hypertension. Enhancement enzymatic function can be effected a class drugs “stimulators,” which depend...

Abstract A novel liposomal formulation was developed for the encapsulation of oligopeptide leuprolide (GlpHisTrpSerTyr‐ D ‐LeuLeuArgProNHEt), a potent analogue gonadotropin releasing hormone used in treatment advanced prostate cancer, endometriosis and precocious puberty. Leuprolide synthesized using solid phase methodology on {3‐[(ethyl‐Fmoc‐amino)‐methyl]‐1‐indol‐1‐yl}‐acetyl AM resin Fmoc/ t Bu chemistry. The new formulation, called ‘liposomes liposomes’ is composed egg...

Abstract: Analogues of GnRH have been widely used in oncology and gynaecology to induce reversible chemical castration. In addition the classic hypophysiotropic action GnRH, it has shown that many malignant cells, such as breast cancer secrete express receptor/s. order study effect modifications position 3 6 on both pituitary binding affinity cell proliferation, we synthesized eight new analogues. All analogues lacked carboxy–terminal Gly 10 –amide an ethylamide residue was added Pro 9 ....

Organic and biological compounds (especially those related to the pharmaceutical industry) have always been of great interest for researchers due their importance development new drugs diagnose, cure, treat or prevent disease. As many API (active ingredients) polymorphs are in nanocrystalline amorphous form blended with polymeric matrix (known as solid dispersion-ASD), structural identification characterization at nm scale conventional X-Ray/Raman/IR techniques becomes difficult. During any...

ABSTRACT Glutathione (GSH) structure‐guided tripeptide analogues were designed and synthesized by solid phase technology, purified (≥95%) RP and/or GF column chromatography, to identify those that, compared with GSH, exhibited similar or higher binding catalytic efficiency toward the MDR‐involved human GSTP1‐1 isoenzyme, could discriminate between allozymic expression products of polymorphic GSTP1 gene locus, designated as hGSTP1*A (Ile 104 /Ala 113 ), hGSTP1*B (Val hGSTP1*C /Val ). The bear...

Sea buckthorn berries (Hippophaë rhamnoides L.) (SB) are considered as a fruit with high nutritional value plethora of bioactive ingredients. The present work focusses on the analysis whole NMR metabolic profile SB grown in an organic orchard Meteora/Greece. In parallel, this study validates/highlights qualitative characteristics osmotic processed according to fresh fruit. composition metabolites was elucidated through sophisticated high-resolution spectroscopy. lipophilic maintains...

ABSTRACT Over the last 20 years, proinsulin C‐peptide emerged as an important player in various biological events. Much time and effort has been spent exploring all functional features of recording its implications Diabetes mellitus. Only a few studies, though, have addressed oligomerization link this procedure with Diabetes. The aim our work was to examine aggregation propensity C‐peptide, utilizing Transmission Electron Microscopy, Congo Red staining, ATR‐FTIR, X‐ray fiber diffraction at...