A5063507385- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
- Lymphatic System and Diseases
- MicroRNA in disease regulation
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- Cancer Mechanisms and Therapy
- Receptor Mechanisms and Signaling
- Fibroblast Growth Factor Research
- Protease and Inhibitor Mechanisms
- RNA modifications and cancer
- Calcium signaling and nucleotide metabolism
- Cellular Mechanics and Interactions
- Blood Coagulation and Thrombosis Mechanisms
- Lipid metabolism and disorders
- Cerebrovascular and genetic disorders
- Cancer Immunotherapy and Biomarkers
- Ion channel regulation and function
- Autophagy in Disease and Therapy
- Estrogen and related hormone effects
- Nanoparticle-Based Drug Delivery
- CAR-T cell therapy research
- Vascular Malformations and Hemangiomas
Texas Tech University
2018-2024
Texas Tech University Health Sciences Center
2018-2024
United States Food and Drug Administration
2023
Center for Drug Evaluation and Research
2023
Abstract Imbalanced angiogenesis is a characteristic of several diseases. Rho GTPases regulate multiple cellular processes, such as cytoskeletal rearrangement, cell movement, microtubule dynamics, signal transduction and gene expression. Among the GTPases, RhoA, Rac1 Cdc42 are best characterized. The role endothelial in embryonic development retinal has been studied, however RhoA yet to be explored. Here, we aimed identify functions, explored compensatory mechanisms. In vitro , involved...
Abstract Lung cancer (LC) is the leading cause of cancer-related deaths. Downregulation CDK1, 4 and 6, key regulators cell cycle progression, correlates with decreased LC proliferation. Enforced expression miRNAs (miRs) a promising approach to regulate genes. Here, we study combinatorial treatment miR-143 miR-506 target 4/6 genes, respectively. We analyzed differential CDK genes by qPCR, western blot, evaluated changes in distribution upon treatment. used an antibody microarray analysis...
Several reports have demonstrated that mouse Cx3cr1 signaling promotes monocyte/macrophage survival. In agreement, we previously found that, in a model of systemic candidiasis, genetic deficiency resulted increased mortality and impaired tissue fungal clearance associated with decreased macrophage We translated this finding by showing the dysfunctional CX3CR1 variant CX3CR1-M280 was risk worse outcome human candidiasis. However, impact mutation on survival is poorly understood. Herein,...
Atezolizumab is an immune checkpoint inhibitor (ICI) targeting PD-L1 for treatment of solid malignancies. Immune checkpoints control the tolerance, and adverse events such as hepatotoxicity induced by ICIs are often considered immune-related event (irAE). However, also highly expressed in normal tissues, e.g., hepatocytes. It still not clear whether, on hepatocytes, atezolizumab may cause damage to liver cells contributing hepatotoxicity. Here, we reveal a novel mechanism which induces human...
Histamine-induced vascular leakage is a core process of allergic pathologies, including anaphylaxis. Here, we show that glycolysis integral to histamine-induced endothelial barrier disruption and hyperpermeability. Histamine rapidly enhanced in cells via pathway involved histamine receptor 1 phospholipase C beta signaling. Consistently, partial inhibition with 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one (3PO) prevented hyperpermeability human microvascular cells, by abolishing the...
The neuronal ceroid lipofuscinoses (NCLs) are a family of rare lysosomal storage disorders. most common form NCL occurs in children harboring mutation the CLN3 gene. This is lethal with no existing cure or treatment beyond symptomatic relief. pathophysiology disease complex and poorly understood, current vivo vitro models failing to identify pharmacological targets for therapeutic intervention. study reports characterization first patient-specific induced pluripotent stem cell (iPSC)-derived...
Lung cancer (LC) is the leading cause of cancer-related deaths worldwide. Similar to other cells, a fundamental characteristic LC cells unregulated proliferation and cell division. Inhibition by halting cycle progression has been shown be promising approach for treatment, including LC. miRNA therapeutics have emerged as important post-transcriptional gene regulators are increasingly being studied use in treatment. In recent work, we utilized two miRNAs, miR-143 miR-506, regulate progression....
The dopaminergic system is involved in the regulation of immune responses various homeostatic and disease conditions. For conditions such as Parkinson's multiple sclerosis (MS), pharmacological modulation dopamine (DA) activity thought to have therapeutic relevance, providing basis for using agents a treatment relevant states. In particular, it was proposed that restoration DA levels may inhibit neuroinflammation. We recently reported new class transporter (DAT) inhibitors with high...
Abstract/Summary The endothelial barrier plays an active role in transendothelial tumor cell migration during metastasis, however, the regulatory elements of this step remain obscure. Here we show that RhoA activation is a determining factor process. Breast cell-induced combined outcome paracrine IL-8-dependent and cell-to-cell contact β 1 integrin-mediated mechanisms, with pathway correlating clinical data. Endothelial-specific blockade or vivo deficiency inhibited metastatic potential...
Lung cancer (LC) is the leading cause of cancer-related deaths worldwide. Similar to other cells, a fundamental characteristic LC cells unregulated proliferation and cell division. Inhibition by halting cycle progression has been shown be promising approach for treatment, including LC. miRNA therapeutics have emerged as important post-transcriptional gene regulators are increasingly being studied use in treatment. In recent work, we utilized two miRNAs, miR-143 miR-506, regulate progression....
Ischaemic retinopathies, such as retinopathy of prematurity (ROP) and diabetic retinopathy, are major causes preventable blindness, which affects a significant number premature infants patients worldwide. Hypoxia-driven release pro-angiogenic growth factors cytokines in the retina leads to endothelial cell proliferation neovascularization, aiming provide oxygen supply oxygen-deprived areas.[1] During this process, usually quiescent cells replicate their deoxyribonucleic acid (DNA) higher...
Abstract Metastasis is the process through which tumor cells disseminate from primary and colonize in distant parts of body. Two steps metastatic are trans-migration cancer endothelial lining blood lymphatic vessels, during entrance (intravasation) exit (extravasation) vascular system. We others have shown that RhoA pathway plays important role permeability. Translating these findings metastasis context we aim to explore signaling on cell trans-endothelial migration metastasis. In vitro,...
Angiogenesis, the formation of new blood vessels, is a highly orchestrated process requiring well‐regulated balance between pro‐angiogenic and anti‐angiogenic factors corresponding signaling networks. It an essential during development in specific adult conditions such as wound healing ovulation. Imbalanced angiogenesis characteristic several diseases cancer, inflammation, rheumatoid arthritis, osteoarthritis age‐related macular degeneration. The Rho family small GTPases plays central role...