A5079279557- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- ATP Synthase and ATPases Research
- Antibiotic Resistance in Bacteria
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Biochemical and Molecular Research
- Helicobacter pylori-related gastroenterology studies
- Immune responses and vaccinations
- Pneumocystis jirovecii pneumonia detection and treatment
- COVID-19 Clinical Research Studies
- Cancer therapeutics and mechanisms
- Respiratory viral infections research
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Influenza Virus Research Studies
- Drug Transport and Resistance Mechanisms
- Virus-based gene therapy research
University of Toronto
2015-2024
Fudan University
2014-2019
State Key Laboratory of Genetic Engineering
2014-2019
Bacille Calmette–Guérin (BCG), an attenuated strain of Mycobacterium bovis, is the only vaccine available for tuberculosis (TB) control. However, BCG not ideal and has two major limitations: exhibits highly variable effectiveness against development TB both in pediatric adult populations can cause disseminated disease immunocompromised individuals. comprises a number substrains that are genetically distinct. Whether how these genetic differences affect efficacy remains largely unknown. In...
Bacterial small regulatory RNAs (sRNAs) are the most abundant class of post-transcriptional regulators and have been well studied in Gram-negative bacteria. Little is known about functions mechanisms sRNAs high GC Gram-positive bacteria including Mycobacterium Streptomyces. Here, we performed an in-depth study 6C sRNA tuberculosis, which conserved among Forty-seven genes were identified as possible direct targets 15 them validated using vivo translational lacZ fusion system. We found that...
Abstract Background The ongoing COVID-19 pandemic has resulted in 185 million recorded cases and over 4 deaths worldwide. Several vaccines have been approved for emergency use humans are being used many countries. However, all the administered by intramuscular injection this may not prevent upper airway infection or viral transmission. Results Here, we describe a novel, intranasally delivered vaccine based on helper-dependent adenoviral (HD-Ad) vector. (HD-Ad_RBD) produces soluble secreted...
The ability of Mycobacterium tuberculosis (M. tb) to survive and persist in the host for decades an asymptomatic state is important aspect pathogenesis. Although adaptation hypoxia thought play a prominent role underlying M. tb persistence, how bacteria achieve this goal largely unknown. Rv0081, member DosR regulon, induced at early stage while Rv3334 one enduring hypoxic response genes. In study, we uncovered genetic interactions between these two transcription factors. RNA-seq analysis...
The type VII secretion system (T7SS) of Mycobacterium tuberculosis secretes three substrate classes: Esx, Esp, and PE/PPE proteins, that play important roles in bacterial physiology host interaction. Five subtypes T7SS, namely ESX-1 to ESX-5, are present M. tb. ESX-4 is the progenitor T7SS but its function not understood. We investigated marinum. show marinum does secrete cognate substrates, EsxT EsxU, under conditions tested. Paradoxically, deletion eccC4, an essential component ESX-4,...
One strategy to develop the next generation of tuberculosis vaccines is construct subunit based on T cell antigens. In this study, we have evaluated vaccine potential a fusion protein combining EsxB, EsxD, EsxG, EsxU, and EsxM Mycobacterium (M. tb). This recombinant protein, named BM, was expressed in purified from Escherichia coli. Immunization C57BL/6 mice with BM formulated Freund's incomplete adjuvant induced production Th1 cytokines (IFN-γ, TNF, IL-2) multifunctional CD4+ cells....
Summary Tuberculosis (TB), the leading cause of death by infectious disease worldwide, is increasingly resistant to first line antibiotics. Developed from a screen against Mycobacterium smegmatis , bedaquiline can sterilize even latent M. tuberculosis infections that may otherwise persist for decades and has become cornerstone treatment multidrug extensively-drug TB. Bedaquiline targets mycobacterial ATP synthase, an essential enzyme in obligate aerobic genus. However, how drug binds intact...
Mycobacterium tuberculosis (Mtb), the causative agent of (TB), can persist in host for decades without causing TB symptoms and cause a latent infection, which is an intricate challenge current control. The DosR regulon, contains approximately 50 genes, crucial non-replicating persistence Mtb. tgs1 one most powerfully induced genes this regulon during Mtb persistence. gene encodes triacyl glycerol synthase catalyzing synthesis (TAG), proposed as energy source bacilli Here, western blotting...
The outbreak of coronavirus disease 19 (COVID-19) has highlighted the demand for vaccines that are safe and effective in inducing systemic airway mucosal immunity against aerosol transmission severe acute respiratory syndrome 2 (SARS-CoV-2). In this study, we developed a novel helper-dependent adenoviral vector-based COVID-19 vaccine encoding full-length SARS-CoV-2 spike protein (HD-Ad-FS). Through intranasal immunization (single-dose prime-boost regimens), demonstrated HD-Ad-FS was...
Abstract The COVID-19 pandemic has affected more than 120 million people and resulted in over 2.8 deaths worldwide. Several vaccines have been approved for emergency use humans are being used many countries. However, all of the administered by intramuscular injection this may not prevent upper airway infection or viral transmission. Here, we describe intranasal immunization a vaccine delivered novel platform, helper-dependent adenoviral (HD-Ad) vector. Since HD-Ad vectors devoid coding...