A5101715526- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Digital Holography and Microscopy
- Optical Coherence Tomography Applications
- Image Processing Techniques and Applications
- Near-Field Optical Microscopy
- Photonic and Optical Devices
- Cell Image Analysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- Optical Systems and Laser Technology
- Advanced X-ray Imaging Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Soil Carbon and Nitrogen Dynamics
- Advanced Fiber Optic Sensors
- Mycorrhizal Fungi and Plant Interactions
- Advanced Optical Sensing Technologies
- Microplastics and Plastic Pollution
- Landslides and related hazards
- Organic Light-Emitting Diodes Research
- Pesticide and Herbicide Environmental Studies
- Cellular Mechanics and Interactions
- CNS Lymphoma Diagnosis and Treatment
- Organic Electronics and Photovoltaics
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial bioremediation and biosurfactants
Renmin Hospital of Wuhan University
2025
Wuhan University
2025
Ningbo University
2023-2024
Zhejiang University
2016-2024
Zhejiang Ocean University
2023
Optica
2018-2023
Huaiyin Normal University
2022
Ningbo University of Technology
2022
State Key Laboratory of Modern Optical Instruments
2016-2018
North University of China
2016-2018
Structured illumination microscopy (SIM) is a powerful technique for super-resolution (SR) image reconstruction. However, conventional SIM methods require high-contrast patterns, which necessitate precision optics and highly stable light sources. To overcome these challenges, we propose new method called contrast-robust structured (CR-SIM). CR-SIM employs deep residual neural network to enhance the quality of imaging, particularly in scenarios involving low-contrast stripes. The key...
Imaging and tracking of near-surface three-dimensional volumetric nanoscale dynamic processes live cells remains a challenging problem. In this paper, we propose multi-color live-cell near-surface-volume super-resolution microscopy method that combines total internal reflection fluorescence structured illumination with multi-angle evanescent light illumination. We demonstrate our approach interference is perfectly adapted to studying subcellular dynamics mitochondria microtubule...
Rhamnolipids have been extensively studied for the remediation of soils contaminated with petroleum hydrocarbons. However, literature on effects rhamnolipids soil microenvironment is scarce. In this study, we adopted a drip irrigation technique to apply rhamnolipid solution planted cotton in saline desert area. The results showed that addition increased organic matter by 5.0–31.6% and reduced electrical conductivity 0.3–42.4%. Additionally, it improved nutrient conditions, reshaped...
Spectral confocal sensors are effective for measuring displacements. The core of the spectral measurement system is a dispersive objective lens that uses optical dispersion to establish one-to-one correspondence between focusing position and wavelength, achieving high-resolution measurements in longitudinal direction. Despite significant progress lenses sensor systems, challenges such as limited range, high cost, insufficient accuracy persist. To expand range improve sensor, we designed...
Measuring three-dimensional nanoscale cellular structures is challenging, especially when the structure dynamic. Owing to informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined offer a axial and subsecond temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution fails characterize depth image densely distributed regions. Here, we emphasize super-resolution MA-TIRF,...
We present an alternative approach to realize structured illumination microscopy (SIM), which is capable for live cell imaging. The prototype utilizes two sets of scanning galvo mirrors, a polarization converter and piezo-platform generate fast shifted, s-polarization interfered periodic variable patterns. By changing the angle galvanometer, we can change position spots at pupil plane objective lens arbitrarily, making it easy switch between widefield total internal reflection...
Structured illumination microscopy (SIM), a super-resolution technology, has wide range of applications in life sciences. In this study, we present an electro-optic high-speed phase-shift imaging system including 2D SIM, total internal reflection fluorescence-SIM, and 3D SIM modes. This uses galvanometers modulator to flexibly quickly control the phase direction structured patterns. Moreover, its design consists precise timing for improved acquisition speed software architecture real-time...
In this study, we developed a novel, compact, and efficient structured illumination microscopy (SIM) system, to our best knowledge. A binary hexagonal lattice pattern was designed implemented on digital micromirror device (DMD), resulting in projection-based structured-light generation. By leveraging the combination of high-speed switching capability DMD with CMOS camera, system can capture 1024×1024 pixels images at 200 fps frame rate when provided sufficient power. The loading reduces...
Cytoskeleton remodeling which generates force and orchestrates signaling trafficking to govern cell migration remains poorly understood, partly due a lack of an investigation tool with high system flexibility, spatiotemporal resolution, computational sensitivity. Herein, we developed multimodal superresolution imaging system–based architecture-driven quantitative (ADQ) framework in spatiotemporal-angular hyperspace enable both identification the optimal mode well-balanced fidelity...
Confocal laser scanning microscopy is one of the most widely used tools for high-resolution imaging biological cells. However, resolution conventional confocal technology limited by diffraction, and more complex optical principles expensive optical-mechanical structures are usually required to improve resolution. This study proposed a deep residual neural network algorithm that can effectively in real time. The reliability real-time performance were verified through experiments on different...
This paper presents design, hardware, software, and parameter optimization for a novel robotic automation system. RABiT is Rapid Automated Biodosimetry Tool high throughput radiological triage. The design considerations guiding the hardware software architecture are presented with focus on methods of communication, ease implementation, need real-time control versus soft time cycles. determination non-contact PVC capillary laser cutting system presented. A approach lymphocyte concentration...
Many techniques using high frequency modulation have been proposed to reduce the effects of 1/f noise in tunable diode-laser absorption spectroscopy (TDLAS). The instruments and devices used by these are not suitable for space applications that require small, low mass power instrumentation. A new estimation technique has already validated two lasers effect at lower frequencies. This paper extends applies it one distribution feedback (DFB) laser diode. In this method a DFB diode is excited...
High throughput has become an important research direction in the field of super-resolution (SR) microscopy, especially improving capability dynamic observations. In this study, we present a hexagonal lattice structured illumination microscopy (hexSIM) system characterized by large view (FOV), rapid imaging speed, and high power efficiency. Our approach employ s spatial light interference to generate two-dimensional SIM pattern, utilize electro-optical modulators for high-speed phase...
We report a series of simulation studies which extends pattern-illuminated Fourier ptychography microscopy by integrating with the nonlinearity arising from saturation fluorophore excited state for super-resolution fluorescence imaging. This extended technique, termed Saturated (SpiFP) microscopy, could achieve resolution four times that wide field when illuminating light intensity approaches threshold in simulations. Increasing leads to further enhancement. In order demonstrate performance...
Reflective confocal imaging systems often face challenges in achieving high resolution, speed, and uniformity, particularly for reflective sample imaging. This study introduces a multi-point system designed to improve expand the field of view, optimize performance using incoherent, telecentric light-emitting diode (LED) illumination. By integrating uniform illumination with microlens arrays optimizing instantaneous structured microscopy optical components, achieves resolution 1116 lp/mm...
We propose a quasi-confocal microscopy autofocus system incorporating an electrically tunable lens (ETL) to achieve differential detection. The ETL changes its focal length collect curves at speeds <300 Hz, allowing selective locking onto desired layers and high-speed operations close the locked plane. By segmenting system's pupil, interference between outgoing incoming near-infrared beams is avoided, thereby greatly improving signal-to-noise ratio. This ultra-sensitive system, with focus...
Measuring the three dimension nanoscale organization of protein or cellular structures is challenging, especially when structure dynamic. Owing to informative total internal reflection fluorescence (TIRF) imaging under varied illumination angles, multi-angle (MA) TIRF has been examined offer a axial and sub-second temporal resolution. However, conventional MA-TIRF still performs badly in lateral resolution fail characterize depth-image densely-distributed regions, leaving huge contrast...