A5063906199- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Photoacoustic and Ultrasonic Imaging
- Near-Field Optical Microscopy
- Digital Holography and Microscopy
- Nanoplatforms for cancer theranostics
- Photonic and Optical Devices
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Luminescence and Fluorescent Materials
- Integrated Circuits and Semiconductor Failure Analysis
- Photoreceptor and optogenetics research
- Autophagy in Disease and Therapy
- Optical Polarization and Ellipsometry
- Hand Gesture Recognition Systems
- Image Processing Techniques and Applications
- Advanced X-ray Imaging Techniques
- Optical and Acousto-Optic Technologies
- Cellular Mechanics and Interactions
- Advanced Materials and Mechanics
- Molecular Biology Techniques and Applications
- Endoplasmic Reticulum Stress and Disease
- Proteoglycans and glycosaminoglycans research
Zhejiang University
2019-2025
Shanghai Ocean University
2024
Huazhong University of Science and Technology
2017-2024
State Key Laboratory of Modern Optical Instruments
2019-2024
Wuhan National Laboratory for Optoelectronics
2017-2024
Shanghai University
2023-2024
Zhejiang Lab
2024
Optica
2022
Ocean University of China
2020
Qingdao Center of Resource Chemistry and New Materials
2020
Characterizing the long-term nanometer-scale interactions between lysosomes and mitochondria in live cells is essential for understanding their functions but remains challenging due to limitations of existing fluorescent probes. Here, we develop cell-permeable organic probes with excellent specificity high photostability. We also use an Atto 647N dye brightness photostability achieve specific labeling cells. Using these probes, obtain dual-color structured illumination microscopy (SIM)...
Observing subcellular structural dynamics in living cells has become the goal of super-resolution (SR) fluorescence microscopy. Among typical SRM techniques, structured illumination microscopy (SIM) stands out for its fast imaging speed and low photobleaching. However, 2D-SIM requires nine raw images to obtain a SR image, leading undesirable artifacts live-cell imaging. In this paper, we propose single-frame (SF-SIM) method based on deep learning that achieves using only single image...
Abstract Computational spectroscopic instruments with broadband encoding stochastic (BEST) filters allow the reconstruction of spectrum at high precision only a few filters. However, conventional design manners BEST are often heuristic and may fail to fully explore potential The parameter constrained spectral encoder decoder (PCSED)—a neural network‐based framework—is presented for in instruments. By incorporating target response definition optical procedures comprehensively, PCSED links...
Super-resolution microscopy has broken the traditional resolution barrier of optical microscopy. However, its application in imaging live and thick specimens been limited. To date, sectioning super-resolution either rely on inaccurate background estimation or hindered live-cell by excessive complexity cost. Here, we report spatial phasor image scanning (spISM), which aims to enhance a factor ∼2 without drawbacks for any microscope equipped with detector array. By incorporating spatial-domain...
Modulation-enhanced localization microscopy (MELM) has demonstrated significant improvements in both lateral and axial precision compared to conventional single-molecule (SMLM). However, modulated illumination based MELM (MELMxy) remains fundamentally limited two-dimensional imaging. Here we present three-dimensional Single-Molecule Modulated Illumination Localization Estimator (SMILE) that synergistically integrates modulation with point spread function engineering. By simultaneously...
Abstract Numerous commercial organic fluorophores with excellent optical properties are precluded from live-cell superresolution imaging due to poor cell permeability. Here, we develop a simple but effective strategy that renders cells permeable cell-impermeable, fluorescent probes by using novel peptide vehicle, PV-1. By coincubation PV-1, 22 different were efficiently delivered into live and specifically labeled variety of organelles. Moreover, PV-1 can simultaneously transfer up three...
Developing a chip-based super-resolution imaging technique with large field-of-view (FOV), deep subwavelength resolution, and compatibility for both fluorescent non-fluorescent samples is desired material science, biomedicine, life researches, etc. Previous on-chip methods focus on either or imaging, putting an urgent requirement the general compatible of them. Here, we introduce universal method based tunable virtual-wavevector spatial frequency shift (TVSFS), realizing labeled label-free...
Imaging three-dimensional, subcellular structures with high axial resolution has always been the core purpose of fluorescence microscopy. However, trade-offs exist between and other important technical indicators, such as temporal resolution, optical power density, imaging process complexity. We report a new modality, interference structured illumination microscopy (FI-SIM), which is based on three-dimensional for wide-field lateral reconstruction. FI-SIM can acquire images quickly within...
Imaging and tracking three-dimensional (3D) nanoscale organizations functions of live cells is essential for biological research but it remains challenging. Among different 3D super-resolution techniques, structured illumination microscopy (SIM) has the intrinsic advantages live-cell studies; based on wide-field imaging does not require high light intensities or special fluorescent dyes to double resolution. However, SIM system developed relatively slowly, especially in imaging. Here, we...
For a long term, spatial resolution of fluorescence microscopy was strictly restricted by the diffraction limit. To solve this problem, various super-resolution technologies have been developed. Super-resolution radial fluctuations (SRRF), an emerging type microscopy, directly analyze raw images and generate results without fluorophore localization, thereby showing more advantages in handling high-density data. Here, speeding up process algorithm with graphics processing unit (GPU)...
Structured-illumination microscopy (SIM) offers a twofold resolution enhancement beyond the optical diffraction limit. At present, SIM requires several raw structured-illumination (SI) frames to reconstruct super-resolution (SR) image, especially time-consuming reconstruction of speckle SIM, which hundreds SI frames. Considering this, we herein propose an untrained neural network (USRNN) with known illumination patterns reduce amount data that is required for by 20 times and thus improve its...
Stimulated emission depletion microscopy (STED) holds great potential in biological science applications, especially studying nanoscale subcellular structures. However, multi-color STED imaging live-cell remains challenging due to the limited excitation wavelengths and large amount of laser radiation. Here, we develop a multiplexed method observe more structures simultaneously with photo-bleaching photo-cytotoxicity. By separating fluorescent probes similar spectral properties using phasor...
Achieving nanometer-scale resolution remains challenging in expansion microscopy due to photon loss. To address this concern, here we develop a multi-color stimulated emission depletion technique based on small-molecule probes realize high labeling density and intensity. Our method substantially lowers the barrier visualizing diverse intracellular proteins their interactions three dimensions. It enables us achieve sub-10-nm structures such as microfilaments, lysosomes, mitochondria,...
Super-resolution optical microscopy is a useful tool to investigate physical and biological characteristics on the sub-100-nanometer scale. Here, we develop pulsed saturated absorption competition (pSAC) break diffraction limit in imaging of series nonbleaching nanoparticles. Based confocal scheme, input laser beam divided into doughnut-shaped pulse Gaussian time-modulated pulse. By adjusting time sequential two pulses, transiently saturates excited-state periphery focal volume, immediately...
Stimulated emission depletion (STED) fluorescence nanoscopy allows the three-dimensional (3D) visualization of nanoscale subcellular structures, providing unique insights into their spatial organization. However, 3D-STED imaging and quantification dense features are obstructed by low signal-to-background ratio (SBR) resulting from optical aberrations out-of-focus background. Here, combining adaptive optics elements, we present an easy-to-implement, flexible, effective method to improve SBR...
Single-molecule localization microscopy (SMLM) gradually plays an important role in deep tissue imaging. However, current SMLM methods primarily rely on fiducial marks, neglecting aberrations introduced by thick samples, thereby resulting decreased image quality tissues. Here, we introduce vectorial situ point spread function (PSF) retrieval (VISPR), a method that retrieves precise PSF model considering both system- and sample-induced under conditions. By employing the theory of maximum...
Cytoskeleton remodeling which generates force and orchestrates signaling trafficking to govern cell migration remains poorly understood, partly due a lack of an investigation tool with high system flexibility, spatiotemporal resolution, computational sensitivity. Herein, we developed multimodal superresolution imaging system–based architecture-driven quantitative (ADQ) framework in spatiotemporal-angular hyperspace enable both identification the optimal mode well-balanced fidelity...
Stimulated emission depletion (STED) nanoscopy is one of the most well-developed techniques that can provide subdiffraction spatial resolution imaging. Here, we introduce dual-modulation difference STED microscopy (dmdSTED) to suppress background noise in traditional By applying respective time-domain modulations two continuous-wave lasers, signals are distributed discretely frequency spectrum and thus obtained through lock-in demodulation corresponding frequencies. The be selectively...
Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required realize cell-permeability and specificity. In this study, we introduce a method overcome these limits—taking advantage of intrinsic affinity bright photostable fluorophores, no matter if they are supposed be live-cell incompatible not. Incubated with cells tissues in particular...