A5034318417- Photoreceptor and optogenetics research
- Pancreatic function and diabetes
- Nanoplatforms for cancer theranostics
- bioluminescence and chemiluminescence research
- Advanced Fluorescence Microscopy Techniques
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Molecular Communication and Nanonetworks
- Protein Kinase Regulation and GTPase Signaling
- Natural Antidiabetic Agents Studies
- ATP Synthase and ATPases Research
- Neuroscience and Neural Engineering
- Cardiovascular Disease and Adiposity
- Cancer Cells and Metastasis
- Polysaccharides and Plant Cell Walls
- Genetics and Neurodevelopmental Disorders
- Muscle activation and electromyography studies
- PI3K/AKT/mTOR signaling in cancer
- Endoplasmic Reticulum Stress and Disease
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Calcium signaling and nucleotide metabolism
- Advanced biosensing and bioanalysis techniques
- Genomics, phytochemicals, and oxidative stress
- Optical Coherence Tomography Applications
Zhejiang University
2018-2025
Guangzhou Medical University
2025
Zhejiang University of Technology
2017-2024
State Key Laboratory of Modern Optical Instruments
2021-2023
Children's Hospital of Zhejiang University
2023
Institute of Pharmacology
2023
Sir Run Run Shaw Hospital
2020
Ministry of Education of the People's Republic of China
2020
The balance of mitochondrial fission and fusion plays an important role in maintaining the stability cellular homeostasis. Abnormal fragmentation have been shown to be associated with oxidative stress, which causes a variety human diseases from neurodegeneration disease cancer. Therefore, induction aggregation may provide alternative approach alleviate these conditions. Here, optogenetic-based system (Opto-MitoA) developed, is based on CRY2clust/CIBN light-sensitive module. Upon blue light...
Our study presents MhOR1, an odorant-responsive chemogenetic tool that facilitates calcium influx upon odorant activation. We optimized its membrane expression and demonstrated ability to regulate insulin release in pancreatic...
Individualized training improved post-stroke motor function rehabilitation efficiency. However, the mechanisms of how individualized facilitates recovery is not clear. This study explored cortical and corticomuscular rehabilitative effects in during training. Sprague-Dawley rats with intracerebral hemorrhage (ICH) were randomly distributed into two groups: forced (FOR-T, n=13) fatigue-controlled (FAT-C, to receive respectively from day 2 14 post-stroke. The FAT-C group exhibited superior...
Coumarins extensively exist in plants and are utilized against diabetes some folk medicines. Recent studies have demonstrated that oxidative stress plays a crucial role the etiology pathogenesis of mellitus. We investigated antioxidant ability 3 coumarins (osthole, esculin, fraxetin) type 2 diabetes. After being fed high-fat diet, ICR mice were exposed to low doses streptozotocin then treated with experimental for 5 weeks. found osthole, metformin significantly lowered fasting blood glucose,...
Abstract Diabetes mellitus (DM) is a common chronic disease affecting humans globally. It characterized by abnormally elevated blood glucose levels due to the failure of insulin production or reduction sensitivity and functionality. Insulin glucagon‐like peptide (GLP)‐1 replenishment improvement resistance are two major strategies treat diabetes. Recently, optogenetics that uses genetically encoded light‐sensitive proteins precisely control cell functions has been regarded as novel...
High-performance biosensors play a crucial role in elucidating the intricate spatiotemporal regulatory roles and dynamics of membrane phospholipids. However, enhancing sensitivity imaging performance remains significant challenge. Here, optogenetic-based strategies are presented to optimize phospholipid biosensors. These involves presequestering unbound cell nucleus regulating their cytosolic levels with blue light minimize background signal interference detection, particularly under...
Background: Traditional rehabilitation with uniformed intensity would ignore individual tolerance and introduce the second injury to stroke survivors due overloaded training. However, effective control of training different is still lacking. The purpose study was investigate rehabilitative effects electromyography (EMG)-based fatigue-controlled treadmill on rat model. Methods: Sprague–Dawley rats after intracerebral hemorrhage EMG electrode implantation surgeries were randomly distributed...
Despite the urgent need to image living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations required realize cell-permeability and specificity. In this study, we introduce a method overcome these limits—taking advantage of intrinsic affinity bright photostable fluorophores, no matter if they are supposed be live-cell incompatible not. Incubated with cells tissues in particular...
Abstract Phospholipase D (PLD) and phosphatidic acid (PA) play a spatio-temporal role in regulating diverse cellular activities. Although current methodologies enable optical control of the subcellular localization PLD by which influence local enzyme activity, overexpression elevates basal activity further leads to increased PA levels cells. In this study, we employed split protein reassembly strategy optogenetic techniques modify superPLD (developed Jeremy Baskin group). We splited variants...
Abstract Despite the urgent needs of imaging living specimens for cutting-edge biological research, most existing fluorescent labeling methods suffer from either poor optical properties or complicated operations to realize cell-permeability and specificity. Here, we introduce a method overcome this tradeoff by incubating cells tissues with dyes, no matter if they are cell-permeable not, at particular conditions (concentration temperature) without physical cell-penetration chemical...
Abstract High-performance biosensors play a crucial role in elucidating the intricate spatiotemporal regulatory roles and dynamics of membrane lipids. However, enhancing their sensitivity substrate-detecting capabilities remains significant challenge. Here, we presented optogenetic-based strategies to optimize phospholipid biosensors. These involved pre-sequestering unbound probes cell nucleus minimize background signals cytoplasm. stored could be released from response blue light according...
Upconversion-mediated optogenetics is an emerging powerful technique to remotely control and manipulate the deep-tissue protein functions signaling pathway activation. This uses lanthanide upconversion nanoparticles (UCNPs) as light transducers through near-infrared (NIR) indirectly activate traditional optogenetic proteins. With merits of high spatiotemporal resolution minimal invasiveness, this enables cell-type specific manipulation cellular activities in deep tissue well living animals....