A5083589430- RNA modifications and cancer
- Advanced Fluorescence Microscopy Techniques
- Cancer-related molecular mechanisms research
- Luminescence and Fluorescent Materials
- Advanced biosensing and bioanalysis techniques
- Advanced Electron Microscopy Techniques and Applications
- RNA Interference and Gene Delivery
- Congenital heart defects research
- MicroRNA in disease regulation
- Cancer-related gene regulation
- Virus-based gene therapy research
- Ubiquitin and proteasome pathways
- Lung Cancer Research Studies
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Colorectal Cancer Screening and Detection
- Peptidase Inhibition and Analysis
- Near-Field Optical Microscopy
- Lipid metabolism and disorders
- Mitochondrial Function and Pathology
- Nanoplatforms for cancer theranostics
Wuhan Blood Center
2023-2024
Wuhan University of Science and Technology
2023-2024
Wuhan National Laboratory for Optoelectronics
2014-2017
Huazhong University of Science and Technology
2014-2017
Characterizing the long-term nanometer-scale interactions between lysosomes and mitochondria in live cells is essential for understanding their functions but remains challenging due to limitations of existing fluorescent probes. Here, we develop cell-permeable organic probes with excellent specificity high photostability. We also use an Atto 647N dye brightness photostability achieve specific labeling cells. Using these probes, obtain dual-color structured illumination microscopy (SIM)...
Abstract Single-molecule localization microscopy (SMLM) achieves super-resolution imaging beyond the diffraction limit but critically relies on use of photo-modulatable fluorescent probes. Here we report a general strategy for constructing cell-permeable organic probes live-cell SMLM by exploiting remarkable cytosolic delivery ability cell-penetrating peptide (rR) 3 R 2 . We develop consisting coupled to cell-impermeable fluorophore and recognition unit. Our results indicate that these are...
Cervical cancer ranks fourth in women terms of incidence and mortality. The RNA-binding protein YTH N6-methyladenosine F2 (YTHDF2) contributes to progression by incompletely understood mechanisms. We show how YTHDF2 controls the fate cervical cells whether could be a valid target for therapy cancer. Sphere formation alkaline phosphatase staining assays were performed evaluate tumor stemness following knockdown. Apoptosis was detected flow cytometry TUNEL assay. compounds 4PBA SP600125 used...
HBV-associated hepatitis B virus x protein (HBx) plays multiple roles in the development of hepatocellular carcinoma. In our prior study, we discovered that miR-187-5p expression was inhibited by HBx. To investigate underlying molecular mechanism HBx-mediated downregulation carcinoma cells, effects HBx and on hepatoma cell were observed, as well their interactions. Through vitro vivo experiments, demonstrated overexpression proliferation, migration, invasion. Simultaneously, observed a...
DNA therapy for cancer requires efficient, selective and safe delivery systems. Compared with other non-viral methods such as lipid or polymer-based vectors, peptide-based systems are biocompatible biodegradable, which leads to lower immunogenicity toxicity. Moreover, peptide vectors easier produce their compositions control because solid-phase synthesis has been extensively developed. However, toward special tumor cells tissues still lacking. In this study, we constructed a 9rR-LTVSPWY...
Characterizing the long-term nanometer-scale interactions between lysosomes and mitochondria in live cells is essential for understanding their functions but remains challenging due to limitations of existing fluorescent probes. Here, we develop cell-permeable organic probes with excellent specificity high photostability. We also use an Atto 647N dye brightness photostability achieve specific labeling cells. Using these probes, obtain dual-color structured illumination microscopy (SIM)...
Numerous new cases of breast cancer are diagnosed among women each year, rendering the most common and rapidly expanding form in females globally. NDUFAF6 encodes a protein that localizes to mitochondria contains predicted phytoene synthase domain. Mutations associated with complex I enzymatic deficiency. This study explored role development prognosis using informatic analysis. Through online website TIMER, we evaluated transcription profile across pan-cancer respectively. We investigated...