NPR1, nonexpressor of pathogenesis-related gene 1, is a master regulator involved in plant defense response to pathogens, and its regulatory mechanism the pathway has been relatively clear. However, information about function NPR1 abiotic stress still limited. Tomato fourth most economically crop worldwide also one best-characterized model plants employed genetic studies. Because lack stable tomato (SlNPR1) mutant, little known SlNPR1 biotic stresses. Here we isolated from 'Ailsa Craig'...

Melatonin acts as a crucial signaling molecule with multiple physiological functions in plant response to abiotic and biotic stresses. However, the impact regulatory mechanism of melatonin on attenuating tomato fruit fungal decay are unclear. In this study, we investigated potential roles modulating resistance Botrytis cinerea explored related molecular mechanisms. The results revealed that disease was strongly enhanced by treatment, 50 μM confirmed best concentration. treatment increased...

Human genetic and genomic studies have supported a strong causal role of SHANK3 deficiency in autism spectrum disorder (ASD). However, the molecular mechanism underlying resulting ASD is not fully understood. Recently, zebrafish has become an attractive organism to model because its high efficiency manipulation robust behavioral phenotypes. The orthologous gene human duplicated genome two homologs, shank3a shank3b. Previous reported shank3 morphants using morpholino method. Here, we report...

Melatonin has various physiological effects, such as the maintenance of circadian rhythms, anti-inflammatory functions, and regulation intestinal barriers. The regulatory functions melatonin in gut microbiota remodeling have also been well clarified; however, role regulating host production remains poorly understood. To address this, we studied contribution to using microbiota-perturbed models. We demonstrated that antibiotic-treated germ-free mice possessed diminished levels serum elevated...

Abstract Exploring electrode materials with larger capacity, higher power density and longer cycle life was critical for developing advanced flexible lithium-ion batteries (LIBs). Herein, we used a controlled two-step method including electrospraying followed calcination treatment by CVD furnace to design novel electrodes of Si/Si x /C Sn/C microrods array consisting nanospheres on carbon cloth substrate (denoted as /C@CC, Sn/C@CC). Microrods composed cumulated (the diameter approximately...

This paper presents the field measurement and evaluation of indoor air quality 25 naturally ventilated residential buildings in northwestern China. Concentrations formaldehyde, benzene, toluene, total volatile organic compounds, nitrogen dioxide PM 2.5 , infiltration rate were measured bedroom, living room kitchen each building different seasons. The results show that concentration formaldehyde can be used as a representative index gaseous pollutants, concentrations various locations highest...

Vanadium oxide-based aqueous zinc-ion batteries exhibit promising potential due to their low cost and safety profiles. However, fabricating cathodes with outstanding electrochemical performance for Zn-ion is still a challenge. Herein, network C@V2O5 materials were prepared using mild chitosan-assisted hydrothermal process. Coin-type cells, as cathode, zinc film an anode, Zn(CF3SO3)2 electrolyte, also assembled, the as-synthesized cathode delivered high specific capacity of 361 mA h g–1 at...

Naturally evolved metabolons have the ability to assemble and disassemble in response environmental stimuli, allowing for rapid reorganization of chemical reactions living cells meet changing cellular needs. However, replicating such capability synthetic remains a challenge due our limited understanding mechanisms by which assembly disassembly naturally occurring multienzyme complexes are controlled. Here, we report synthesis chemical- light-responsive protein cages assembling metabolons,...

As one of the most important trace elements required by sows, especially in late gestation period, iron plays a crucial role growth and development fetus. To explore effects dietary supplementation with ferrous sucrose on reproductive performance sows hepatic stores offspring, sixty primiparous Landrace × Yorkshire day 95 an average body weight 174.1 ± 7.7 kg were randomly assigned to two groups basic diet (control) supplemented 109 mg/kg (FS) fully randomized block design. The trial lasted...

Foodborne bacteria, particularly Escherichia coli (E. coli) O157:H7, are significant contributors to foodborne illnesses, with antibiotic overuse exacerbating the issue through emergence of multidrug-resistant strains. This study investigated potential E. phages in food safety, examining their biological traits and bacteriostatic properties. Two (vB_EcoP_SD2, vB_EcoP_SD6) O157:H7 were isolated from slaughterhouse sewage characterized for morphology, genomic composition, phage phylogenetic...

Background: Plant essential oil is used as a feed additive instead of antibiotics, but there little research on its application in ruminants. Therefore, it very necessary to study the effects growth performance, rumen fermentation and nutrient digestibility Hu sheep. Methods: 96 weaned male sheep, weighing 17.69±0.20 kg, were randomly divided into 3 groups (control, 300 mg/kg 600 oil), with 4 replicates per group 8 sheep replicate. The pre-feeding period was 10 days full trial 61 days....

China’s pilot free trade zones play an important role in promoting deep-level reform and high-standard opening up. Based on the panel data of 284 prefecture-level cities China from 2009 to 2021, article explores impact urban innovation using multi-period difference-in-differences model, mediation effect spatial treating zone as a quasi-natural experiment. The study shows following: establishment boosts sustained growth innovation, results still hold after series robustness tests; enabling is...

Self-assembled protein cages are attractive scaffolds for organizing various proteins of interest (POIs) toward applications in synthetic biology and medical science. However, specifically attaching multiple POIs to a single cage remains challenging, resulting diversity among the functionalized particles. Here, we present engineering self-assembled cage,

As a direction in the development of computer vision, fog visibility detection is very important for traffic safety. Aiming at problem appearing highspeed road traffic, this paper proposes level method based on image HSV color histogram. First, convert background space from RGB to space. And then achieve by classifying weathers different into types using histogram features (including H, S, and V) various weather conditions. The experimental results show that can make qualitative judgments...

The cell adhesion molecule L1-like (CHL1 or CALL) gene is located on chromosome 3p26.3, and it highly expressed in the central peripheral nervous systems. protein encoded by this a member of L1 family neural molecules, plays role system development synaptic plasticity. Moreover, studies mice have revealed that CHL1 prime candidate for dosage-sensitive autosomal form mental retardation. To date, four patients with microdeletion two microduplication 3p26.3 encompassing only been reported...

Various fluorescent sensing systems for miRNA detection have been developed, but they mostly contain enzymatic amplification reactions and label procedures. The strict reaction conditions of tool enzymes the high cost labeling limit their potential applications, especially in complex biological matrices. Here, we addressed difficult problems report a strategy label-free DNA dendrimers based on enzyme-free nonlinear hybridization chain (HCR)-mediated multiple G-quadruplex simple, sensitive,...

Recently, deleterious variants in the BR serine/threonine kinase 2 (BRSK2) gene have been reported patients with autism spectrum disorder (ASD), suggesting that BRSK2 is a new high-confidence ASD risk gene, which presents an opportunity to understand underlying neuropathological mechanisms of ASD. In this study, we performed clinical and neurobehavioral evaluations proband de novo non-sense variant (p.R222X) other mutant patients. To validate as generated novel brsk2b-deficient zebrafish...

Improving the supply chain resilience of mineral resources industry is crucial for ensuring national economic security in China. Based on and demand data China’s from 2002 to 2018, this study adopts system dynamics model simulate industry, mining smelting processing under scenario steady development crisis. From simulation results, reserves two scenarios are nearly same, indicating that they weakly affected by foreign market, both have strong resilience. The has a high dependence imports...

SHANK3 is a scaffolding protein that binds to various synaptic proteins at the postsynaptic density (PSD) of excitatory glutamatergic synapses. not only strongly implicated in autism spectrum disorders (ASD) but also plays critical role human Phelan-McDermid syndrome (22q13.3 deletion syndrome). Accumulated experimental evidence demonstrates zebrafish model system useful for studying functions ASD-related gene during early development. However, many basic features shank3 transcript...