A5061167335- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Pancreatic function and diabetes
- Pediatric Hepatobiliary Diseases and Treatments
- Liver Diseases and Immunity
- Endoplasmic Reticulum Stress and Disease
- Drug-Induced Hepatotoxicity and Protection
- Pancreatitis Pathology and Treatment
- Lymphoma Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Skin and Cellular Biology Research
- Phagocytosis and Immune Regulation
- Animal Genetics and Reproduction
- Gastrointestinal Tumor Research and Treatment
- Diabetes and associated disorders
- Alcohol Consumption and Health Effects
- Gallbladder and Bile Duct Disorders
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cell death mechanisms and regulation
- Intramuscular injections and effects
- Esophageal and GI Pathology
- Potassium and Related Disorders
- Advanced Glycation End Products research
- Nuclear Structure and Function
- Signaling Pathways in Disease
Indiana University School of Medicine
2011-2024
Indiana University Health
2023-2024
Riley Hospital for Children
2024
Indiana University
2015-2024
Indiana University – Purdue University Indianapolis
2011-2023
Richard L. Roudebush VA Medical Center
2016
University School
2015
Mayo Clinic in Arizona
2009-2012
Mayo Clinic
2008-2011
University of Alabama at Birmingham
2002-2004
Free fatty acid induction of inflammation and cell death is an important feature nonalcoholic steatohepatitis (NASH) has been associated with disruption the endoplasmic reticulum activation unfolded protein response (UPR). After chronic UPR activation, transcription factor CHOP (GADD153/DDIT3) triggers death; however, mechanisms linking or to hepatoceullular injury in pathogenesis NASH are not well understood. Using HepG2 primary human hepatocytes, we found that induces inflammatory...
The pancreatic beta-cell-specific expression of the insulin gene is mediated, at least in part, by interaction unique trans-acting beta-cell factors with a cis-acting DNA element found within enhancer (5'-GC CATCTG-3'; referred to as control [ICE]) present rat II between positions -100 and -91. This sequence contains consensus binding site for group DNA-binding transcription called basic helix-loop-helix proteins (B-HLH). As consequence similarity ICE motif associated elements B-HLH class...
Saturated free fatty acids (FFA) induce hepatocyte lipoapoptosis, a key mediator of liver injury in nonalcoholic disease (NAFLD). Lipoapoptosis involves the upregulation BH3-only protein PUMA, potent pro-apoptotic protein. Given that dysregulation hepatic microRNA expression has been observed NAFLD, we examined role miRNA regulating PUMA during lipotoxicity. By silico analysis, identified two putative binding sites for miR-296-5p within 3′ untranslated region (UTR) mRNA. Enforced levels...
The contribution of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a death expressed by cells the innate immune system, to cholestatic liver injury has not been explored. Our aim was ascertain if TRAIL contributes in bile duct-ligated (BDL) mouse. C57/BL6 wild-type (wt), heterozygote (TRAIL(+/-)), and knockout (TRAIL(-/-)) mice were used for these studies. Liver fibrosis examined 7 14 days after BDL, respectively. Hepatic messenger RNA (mRNA) 6-fold greater BDL animals...
The mechanisms of free fatty acid-induced lipoapoptosis are incompletely understood. Here we demonstrate that Mcl-1, an anti-apoptotic member the Bcl-2 family, was rapidly degraded in hepatocytes response to palmitate and stearate by a proteasome-dependent pathway. Overexpression ubiquitin-resistant Mcl-1 mutant Huh-7 cells attenuated palmitate-mediated loss lipoapoptosis; conversely, short hairpin RNA-targeted knockdown sensitized these lipoapoptosis. Palmitate-induced degradation novel...
Background Ossabaw miniature swine when fed a diet high in fructose, saturated fat and cholesterol (NASH diet) develop metabolic syndrome nonalcoholic steatohepatitis (NASH) characterized by liver injury fibrosis. This study was conducted to further characterize the development of NASH this large animal model. Methods were standard chow (control group; n = 6) or (n for 24 weeks. Blood tissue collected histology at 0, 8, 16 weeks dietary intervention. Hepatic apoptosis lipid levels assessed...
Nonalcoholic fatty liver disease (steatosis) is the most prevalent in Western world. One of advanced pathologies nonalcoholic steatohepatitis (NASH), which associated with induction unfolded protein response (UPR) and disruption autophagic flux. However, mechanisms by these processes contribute to pathogenesis human diseases are unclear. Herein, we identify α isoform inhibitor Bruton's tyrosine kinase (IBTKα) as a member UPR, whose expression preferentially translated during endoplasmic...
The pancreatic beta-cell-specific expression of the insulin gene is mediated, at least in part, by interaction unique trans-acting beta-cell factors with a cis-acting DNA element found within enhancer (5'-GC CATCTG-3'; referred to as control [ICE]) present rat II between positions -100 and -91. This sequence contains consensus binding site for group DNA-binding transcription called basic helix-loop-helix proteins (B-HLH). As consequence similarity ICE motif associated elements B-HLH class...
Selective transcription of the insulin gene in pancreatic beta cells is regulated by its enhancer, located between nucleotides -340 and -91 relative to start site. Transcription from enhancer controlled both positive- negative-acting cellular factors. Cell-type-specific expression mediated principally a single cis-acting element -100 rat II (referred as control [ICE]), which acted upon these activities. Analysis effect 5' deletions within has identified region -217 -197 that also site...
Aim Serum high mobility group box 1 protein (HMGB1) is a proinflammatory molecule that could potentially serve as biomarker for non-alcoholic fatty liver disease (NAFLD) and steatohepatitis (NASH) due to its correlation with degree of fibrosis. The aim the current study was examine cross-sectional longitudinal relationships between serum HMGB1 levels histology in adults children NAFLD participating two large randomized controlled trials. Methods were measured at various time points NAFLD,...
Hospital readmissions are common in patients with cirrhosis, but there few studies describing readmission preventability. We aimed to describe the incidence, causes, and risk factors for preventable this population.
Endoscopic ultrasound (EUS)-guided injection of cyanoacrylate (CYA) for primary prophylaxis (PP) gastric varices (GV) is controversial. This study evaluates the safety and efficacy this intervention.