A5035985168- Liver Disease Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Hepatitis B Virus Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Hepatitis C virus research
- Eosinophilic Esophagitis
- Diet, Metabolism, and Disease
- Gastroesophageal reflux and treatments
- Cell death mechanisms and regulation
- Liver Diseases and Immunity
- Liver Disease and Transplantation
- Endoplasmic Reticulum Stress and Disease
- Gastrointestinal motility and disorders
- Liver physiology and pathology
- HIV, Drug Use, Sexual Risk
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Systemic Sclerosis and Related Diseases
- Child Nutrition and Feeding Issues
- Gastric Cancer Management and Outcomes
- Genetic and Kidney Cyst Diseases
- Pharmaceutical industry and healthcare
- Pancreatitis Pathology and Treatment
- Delphi Technique in Research
National University of Misiones
2014-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2024
Centro Científico Tecnológico - San Juan
2017-2018
Hospital Británico de Buenos Aires
2014
University of Buenos Aires
2008-2014
Mayo Clinic
2007-2008
Mayo Clinic in Arizona
2008
Abstract Background & Aims Hepatocyte apoptosis, the hallmark of non‐alcoholic steatohepatitis ( NASH ) contributes to liver injury and fibrosis. Although, both intrinsic extrinsic apoptotic pathways are involved in pathogenesis , final common step apoptosis is executed by a family cysteine‐proteases termed caspases. Thus, our aim was ascertain if administration Emricasan, pan‐caspase inhibitor, ameliorates fibrosis murine model . Methods C57/ BL 6J‐mice were fed regular chow or high fat...
Elevated circulating free fatty acids (FFA) contribute to the development of hepatic steatosis and promote hepatocyte apoptosis by incompletely defined mechanisms. Although death ligand TRAIL has been implicated in a variety pathological liver diseases, role mediating FFA induced steatotic hepatocytes is unknown.We examined cytotoxicity an vitro model FFA.Hepatocytes (Huh 7 cells, HepG2 primary rat hepatocytes) were rendered incubation with oleic acid. Apoptosis was assessed morphologically...
Hepatocyte lipoapoptosis, a critical feature of nonalcoholic steatohepatitis, can be replicated in vitro by incubating hepatocytes with saturated free fatty acids (FFA). These toxic FFA induce Bim expression, which is requisite for their cytotoxicity. Because the FoxO3a transcription factor has been implicated our aim was to determine if FoxO3a-dependent mechanism. In Huh-7 cells, FFA, palmitic and stearic acid, increased mRNA 16-fold. Treatment cells induced dephosphorylation (activation)...
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death worldwide. Most studies addressing epidemiology HCC originate from developed countries. This study reports preliminary findings a multinational approach to characterize in South America.We evaluated 1336 patients seen at 14 centres six American countries using retrospective design with participating completing template chart patient characteristics. The diagnosis was made radiographically or histologically for...
The contribution of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a death expressed by cells the innate immune system, to cholestatic liver injury has not been explored. Our aim was ascertain if TRAIL contributes in bile duct-ligated (BDL) mouse. C57/BL6 wild-type (wt), heterozygote (TRAIL(+/-)), and knockout (TRAIL(-/-)) mice were used for these studies. Liver fibrosis examined 7 14 days after BDL, respectively. Hepatic messenger RNA (mRNA) 6-fold greater BDL animals...
Cut-points for non-invasive tests (NITs) risk stratification in metabolic dysfunction-associated steatotic liver disease (MASLD) were derived from predominantly non-Hispanic populations. It is unknown if these cut-points perform adequately Hispanic individuals. We assessed the performance characteristics of current NIT among patients and determined whether they could be further optimized.
Recent studies have suggested an association between H. pylori and metabolic-disfunction associated fatty liver disease (MASLD). However, epidemiologic yielded inconsistent results. We aim to evaluate the of G-allele PNPLA3 in MASLD diagnosis, markers severity.
In the metabolic syndrome with hyperinsulinemia, mitochondrial inhibition facilitates muscle fat and glycogen accumulation accelerates its progression. last decade, nitric oxide (NO) emerged as a typical modulator by reversibly inhibiting citochrome oxidase oxygen utilization. We wondered whether insulin-operated signaling pathways modulate respiration via NO, to alternatively release complete glucose oxidation CO(2) H(2)O or drive storage glycogen.We illustrate here that NO produced...
We aim to describe the efficacy, safety profile, and variables associated with survival in patients hepatocellular carcinoma (HCC) treated sorafenib South America.Sorafenib has been shown improve advanced HCC. There are few data on use for HCC America.We performed a retrospective analysis of cases from 8 medical centers 5 American countries, between January 2010 June 2017. The primary endpoint was overall (OS), which defined as time initiation death or last follow-up. Risk factors decreased...
Summary Background Ursodeoxycholic acid (UDCA) is the first‐line therapy for primary biliary cholangitis (PBC). However, nearly 40% of patients have an incomplete response to UDCA. The addition bezafibrate has shown biochemical benefit in this group patients. Aim To evaluate long‐term effects UDCA combination with on histological outcomes UDCA‐refractory PBC. Methods Fifty‐nine refractory were included. Clinical parameters monitored and paired liver biopsy (PLB) was performed after 5 years...
Obesity arises on defective neuroendocrine pathways that increase energy intake and reduce mitochondrial metabolism. In the metabolic syndrome, dysfunction accomplishes defects in fatty acid oxidation reciprocal triglyceride content with insulin resistance hyperglycemia. Mitochondrial inhibition is attributed to reduced biogenesis, excessive fission, low adipokine-AMP-activated protein kinase (AMPK) level, but lateness of respiratory chain contributes perturbations. Considering nitric oxide...
Recent studies have suggested an association between H. pylori and metabolic dysfunction associated steatotic liver disease (MASLD). We aim to evaluate the of virulence genes with non-invasive markers injury fibrosis in MASLD subjects.
The ECHO model was developed to expand access medical care for populations with HCV infection in underserved areas. We aimed compare treatment outcomes community-based clinics the Austral University Hospital (AUH) and assess improvement physician knowledge skills. In October 2015, we established an clinic at AUH Buenos Aires. To evaluate impact of this programme, conducted a prospective cohort study comparing healthcare providers from different Argentinean provinces. A survey evaluating...
There is scarce data pertaining to acute hepatitis C (aHC) infection in South America. We aimed describe clinical characteristics and evolution of aHC a American cohort. A retrospective survey was conducted at 13 hepatology units. All patients ≥16 years old with diagnosis were included. Demographic, outcome information registered standardized ad hoc questionnaire. Sixty-four The majority middle-aged (median age: 46 years) female (65.6%); most them symptomatic (79.6%). HCV-1 the prevalent...
Abstract Background Functional dyspepsia (FD) is a multifactorial disorder. Helicobacter pylori ( H. )‐related (HpD) may be considered separate entity. Duodenal eosinophilia potential pathogenic mechanism in FD. However, the impact of duodenal and host genetic polymorphism innate pro‐inflammatory cascade, nucleotide‐binding oligomerization domain 1 (NOD‐1), interleukin‐1 beta (IL‐1β) HpD was not explored. Aim To evaluate association NOD1 ‐796G>A IL‐1B ‐511C>T gene variants low‐grade...
Induction of hepatic stellate cell (HSC) apoptosis is a viable therapeutic strategy to reduce liver fibrogenesis. Although BH3-only proteins the Bcl-2 family trigger pro-apoptotic pathways, mediating HSC have not been well defined. Our aim, using proteasome inhibition as model induce apoptosis, was examine contributing death this key subtype.Apoptosis induced by treating LX-2 cells, an immortalized human line, and primary rat cells with inhibitor MG-132.Treatment inhibitors increased...
Functional dyspepsia (FD) is a multifactorial disorder. Helicobacter pylori (H. pylori)-related (HpD) may be considered separate entity. Duodenal eosinophilia potential pathogenic mechanism in FD. However, the impact of duodenal and H. virulence genes HpD was not explored. We aim to evaluate association low-grade HpD.A multi-center cross-sectional study conducted. A total 301 patients who meet Rome-III criteria were selected before upper endoscopy, 95 included after normal endoscopy positive...
Recent studies have suggested an association between Helicobacter pylori (Hpyl) and non-alcoholic fatty liver disease (NAFLD). The current study aimed to examine the of Hpyl virulence genes NAFLD in dyspeptic patients. prospective multicenter from 2019 2022 northeast Argentina. We evaluated 386 patients who fulfilled ROME III criteria underwent gastroscopy. was defined by ultrasound absence other known diseases. cagA, vacAs1/s2, vacAm1/m2 were analyzed PCR. prevalence 41% (156/383), no with...
The pathophysiology of NAFLD is only partially unrevealed; it considered as a multifactorial disorder, attributed to multiple, parallel "hits," both genetic and environmental. It has been described that the single nucleotide polymorphism at rs738409 in PNPLA3 gene strongly associated with hepatic steatosis its progression. Conversely, H. pylori infection related metabolic syndrome, type-2 diabetes mellitus, dyslipidemia, which are known risk factors for NAFLD. However, evaluation Infection...