A5077023503- Exercise and Physiological Responses
- Muscle metabolism and nutrition
- Estrogen and related hormone effects
- Pancreatic function and diabetes
- Inflammatory mediators and NSAID effects
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- High Altitude and Hypoxia
- Adrenal Hormones and Disorders
- Biochemical effects in animals
- Hormonal Regulation and Hypertension
- Cardiovascular and exercise physiology
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Psoriasis: Treatment and Pathogenesis
- Genetics and Physical Performance
- NF-κB Signaling Pathways
- Telomeres, Telomerase, and Senescence
- Eicosanoids and Hypertension Pharmacology
- Peptidase Inhibition and Analysis
- IL-33, ST2, and ILC Pathways
- Redox biology and oxidative stress
- Adenosine and Purinergic Signaling
- Phagocytosis and Immune Regulation
- Heat shock proteins research
Showa University
2015-2025
Shriners Hospitals for Children - Erie
2011-2013
Harvard University
2013
Massachusetts General Hospital
2013
International Pacific University
2012
RIKEN
2003-2005
Here we report the molecular identification of membrane-bound glutathione (GSH)-dependent prostaglandin (PG) E2 synthase (mPGES), a terminal enzyme cyclooxygenase (COX)-2-mediated PGE2 biosynthetic pathway. The activity mPGES was increased markedly in macrophages and osteoblasts following proinflammatory stimuli. cDNA for mouse rat mPGESs encoded functional proteins that showed high homology with human ortholog (microsomal glutathioneS-transferase-like 1). expression induced by stimuli...
Here we report the molecular identification of cytosolic glutathione (GSH)-dependent prostaglandin (PG) E2 synthase (cPGES), a terminal enzyme cyclooxygenase (COX)-1-mediated PGE2 biosynthetic pathway. GSH-dependent PGES activity in cytosol rat brains, but not other tissues, increased 3-fold after lipopolysaccharide (LPS) challenge. Peptide microsequencing purified revealed that it was identical to p23, which is reportedly weakly bound component steroid hormone receptor/hsp90 complex....
We have recently shown that two distinct prostaglandin (PG) E<sub>2</sub> synthases show preferential functional coupling with upstream cyclooxygenase (COX)-1 and COX-2 in PGE<sub>2</sub> biosynthesis. To investigate whether other lineage-specific PG also either COX isozyme, we introduced these enzymes alone or combination into 293 cells to reconstitute their interrelationship. As did the membrane-bound synthase, perinuclear thromboxane synthase PGI<sub>2</sub> generated respective products...
In this study we report the cloning and characterization of a novel human aminopeptidase, which designate leukocyte-derived arginine aminopeptidase (L-RAP). The sequence encodes 960-amino acid protein with significant homology to placental leucine adipocyte-derived aminopeptidase. predicted L-RAP contains HEXXH(X)18E zinc-binding motif, is characteristic M1 family zinc metallopeptidases. Phylogenetic analysis indicates that forms distinct subfamily in family. Immunocytochemical located...
Human plasma contains at least three forms of adiponectin: a trimer, hexamer, and high-molecular-weight (HMW) multimer. We purified HMW adiponectin from human using its affinity to gelatin obtained monoclonal antibodies against it. On Western blot analysis, the reactivity these was shown be restricted non-heat-denatured form molecules. heating, collagen-like domain molecules became denatured, thus trimer could not maintained. From these, were suggested react with intact adiponectin. With...
T cells contribute to immunotherapy and autoimmune pathogenesis Langerhans (LCs) have a substantial ability activate cells. In vitro -generated monocyte-derived LCs (Mo-LCs) are useful models study LC function in diseases test future LC-based immunotherapies. Although dendritic (DCs) expressing high levels of Delta-like 4 (DLL4 + DCs), which is member the Notch ligand family, greater than DLL4 − DCs cells, induction method human has yet be determined. The aim this establish whether Mo-LCs...
Cytosolic phospholipase A2(cPLA2) α plays critical roles in lipid mediator synthesis. We performed far-Western analysis and identified a 60-kDa protein (P60) that interacted with cPLA2α Ca2+-dependent manner. Peptide microsequencing revealed purified P60 was identical to vimentin, major component of the intermediate filament. The interaction occurred between C2 domain head vimentin. Immunofluorescence microscopic demonstrated vimentin colocalized around perinuclear area cPLA2α-overexpressing...
cPGES [cytosolic PG (prostaglandin) E synthase] is constitutively expressed in various cells and can regulate COX (cyclo-oxygenase)-1-dependent immediate PGE2 generation. In the present study, we found that underwent serine phosphorylation, which was accelerated transiently after cell activation. Several lines of evidence suggest a cPGES-activating protein kinase CK-II (casein II). Recombinant phosphorylated directly by associated with vitro, resulting marked reduction Km for substrate PGH2....
Although many single nucleotide polymorphism (SNP) studies have reported an association of atopy, allergic diseases and total serum immunoglobulin E (IgE) levels, almost all these sought risk factors for the onset diseases. Furthermore, analyzed a gene hardly any environmental factors. In analyses, results could be masked effects other genes may decreased. Here, we described correlation between four [interleukin (IL)-4 (C-590T), IL-4 receptor (A1652G), FCER1B (G6842A) STAT6 (G2964A)] in...
Nitric oxide (NO) has been implicated in pancreatic β-cell death the development of diabetes. The mechanisms underlying NO-induced have not clearly defined. Recently, receptor-interacting protein-1 (RIP1)-dependent necrosis, which is inhibited by necrostatin-1, an inhibitor RIP1, emerged as a form regulated necrosis. Here, we show that NO donor-induced was necrostatin-1. Unexpectedly, however, RIP1 knockdown neither cell nor altered protective effects necrostatin-1 donor-treated β-cells....
Insulin receptor substrate-2 (IRS-2) plays a critical role in the survival and function of pancreatic β-cells. Gene disruption IRS-2 results failure β-cell compensatory mechanism diabetes. Nonetheless, regulation protein expression β-cells remains largely unknown. Inducible nitric-oxide synthase (iNOS), major mediator inflammation, has been implicated damage type 1 2 The effects iNOS on have not yet investigated Here, we show that NO donor decreased INS-1/832 insulinoma cells mouse islets,...
The leukocyte‐derived arginine aminopeptidase (L‐RAP) is the second localized in endoplasmic reticulum (ER) processing antigenic peptides presented to major histocompatibility complex (MHC) class I molecules. In this study, genomic organization of gene encoding human L‐RAP was determined and regulatory mechanism its expression elucidated. entire structure similar both placental leucine ( P‐LAP ) adipocyte‐derived A‐LAP genes, confirming close relationship these three enzymes. Interferon...
Math2 (NEX-1/NeuroD6) is a member of the basic helix-loop-helix transcription factor family and involved in neuronal differentiation maturation. In this study, we identified genes targeted by using DNA microarrays cultured rat cortical cells transfected with Math2. Of regulated Math2, focused on plasticity-related gene 1 (Prg1). Prg1 expression induced was confirmed PC12 analyzed real-time quantitative PCR. promoter region Prg1, four E-boxes [designated -E1 to -E4 (CANNTG)] recognized...
Recent studies have highlighted the pathogenic roles of IL-17-producing CD8+ T cells (T-cytotoxic 17 [Tc17]) in psoriasis. However, underlying mechanisms Tc17 induction remain unclear. In this study, we focused on subsets Th17 and their mechanism promotion responses. We determined that Th17-enriched fraction expressed melanoma cell adhesion molecule (MCAM) CCR6, but not CD161, because subset produced IL-17A abundantly presence these peripheral blood patients has been correlated with severity...
Angiotensin III (Ang III) is a heptapeptide derived from Ang II that has been confirmed as the preferred agonist of angiotensin type 2 receptor (AT2R). Recent studies have revealed AT2R mainly exerts anti-inflammation effects. However, effects III/AT2R pathway on adipocytes remain unknown. Here, glucose uptake were examined. The results showed expression was upregulated during adipogenesis in 3T3-L1 preadipocytes, whereas AT1R diminished. Also, (10 nM) significantly increased by adipocytes,...
Although antigen-specific T helper (Th) cells are developed from naive cells, human Th17 not derived CD4+ unlike murine cells. Therefore, the source of has remained unresolved. In this study, we assessed early differentiation pathway CD31+ thymic into stem cell memory CCR6+ precursors and regulation process by cytokines. Peripheral blood mononuclear were isolated healthy volunteers. We found that only CD31- type had ability to produce IL-17A in response Th17-inducing stimuli. A tracking...
The treatment of acute ischemic stroke usually involves argatroban administration by continuous infusion for 2 d and intravenous twice a day 5 after that. However, the appropriate dose to be administered is not clear. Therefore, no studies have been reported comparison 3 patients. We aimed identify connection between differences in worsening symptoms retrospectively evaluated data 107 patients who received with argatroban. study endpoint was defined as from days 7. Logistic regression...
Monocyte-derived Langerhans cell-like dendritic cells （Mo-LCs） are involved in epidermal disorders such as psoriasis murine models. However, the roles of Mo-LCs pathogenesis humans remain unclear. Also, contribution notch ligand delta-like 1 （DLL-1）, expressed on keratinocytes, to Mo-LC functions requires clarification. Here, we established a new method stimulating derived from CD14＋ monocytes with immobilized human DLL-1 generate induced （DI（＋）Mo-LCs）. The DI（＋）Mo-LCs were compared （Mo-DCs）...
The purpose of the study was to clarify effects follicular and luteal phases on urinary levels urokinase plasminogen activator receptor (uPAR) following 2 hours moderate cycling exercise in premenopausal women. Recreationally active women (n=12; age: 20.9±0.3 year; height: 160.1±5.6 cm; body weight: 54.7±5.8 kg; mass index: 21.3±1.8 kg/m2; fat: 21.7±3.0 % [mean±SD]) served as subjects. Before (Pre) after (immediately Post 24 hours) at 60%VO2peak, spot urine samples were taken for later...