Exploring the ratiometric fluorescence biosensing of DNA-templated biemissive silver nanoclusters (AgNCs) is significant in bioanalysis, yet design a stimuli-responsive DNA device challenge. Herein, using anti-digoxin antibody (anti-Dig) with two identical binding sites as model, tweezer-like architecture assembled to populate fluorescent green- and red-AgNCs (g-AgNCs r-AgNCs), aiming produce ratio signal via specific recognition anti-Dig haptens (DigH). To this end, four probes are...

Engineering of multivariate biosensing and imaging platforms involved in disease plays a vital role effectively discerning cancer cells from normal facilitating reliable targeted therapy. Multiple biomarkers such as mucin 1 (MUC1) nucleolin are typically overexpressed breast compared to human epithelium cells. Motivated by this knowledge, dual-responsive DNA tetrahedron nanomachine (drDT-NM) is constructed through immobilizing two recognition modules, MUC1 aptamer (MA) hairpin H1* encoding...

To address the limitations of typical hairpin-structural molecular beacons, exploring ability a quasi-molecular beacon (qMB) to create label-free fluorescence biosensors is intriguing and remains challenge. Herein, we propose first example modular qMB with feature stimulation-responsive conformation switch develop an aggregated Ag nanocluster (aAgNC) in bifurcated DNA scaffold for fluorescently sensing specific initiator (I*). This was well designed program four functional modules:...

Dark or weak-emissive DNA-harbored silver nanoclusters (AgNCs) can be remarkably lighted up when approaching to guanine bases. The resultant bright AgNCs acting as a fluorescent reporter are fascinating in biosensing. To explore the applicable guanine-enhanced emission of for biosensing microRNA-155 (miR-155) model, here we designed unique stem–loop hairpin beacon (HB) encoding with an miR-155-recognizable sequence and AgNCs-nucleable template, well helper tethering partially locked...

Proximity-localized catalytic hairpin assembly (plCHA) is intriguing for rapid and sensitive assay of an HIV-specific DNA segment (T*). Using template-integrated green Ag nanoclusters (igAgNCs) as emitters, herein, we report the first design a T*-activated plCHA circuit that confined in three-way-junction architecture (3WJA) fluorescence sensing T*. To this end, T*-recognizable complement programmed stem-loop (H1), two split template sequences igAgNCs are separately overhung contiguous to...

The DNA frame structure as a natural shell to stably shield the sequence-templated Ag nanocluster core (csAgNC) is intriguing yet challenging for applicable fluorescence biosensing, which elaborate programming of cluster scaffold inside DNA-based cage guide csAgNC nucleation might be crucial. Herein, we report first design symmetric tetrahedral nanocage (TDC) that was self-assembled in one-pot process using C-rich template strand and four single strands. Inside as-constructed soft TDC...

The reaction kinetics and yield of traditional DNA assembly with a low local concentration in homogeneous solution remain challenging. Exploring confined catalytic (CCDA) is intriguing to boost the rate efficacy for creating rapid sensitive biosensing platforms. A rolling circle amplification (RCA) product containing multiple tandem repeats natural scaffold capable guiding periodic customized functional probes at precise sites. Here, we present RCA-confined CCDA strategy speed up amplifiable...

Developing a DNA autocatalysis-oriented cascade circuit (AOCC) via reciprocal navigation of two enzyme-free hug-amplifiers might be desirable for constructing rapid, efficient, and sensitive assay-to-treat platform. In response to specific trigger (T), seven functional hairpins were designed execute three-branched assembly (TBA) three isotropic hybridization chain reaction (3HCR) events operating the AOCC. This was because new inducers reconstructed in TBA arms initiate 3HCR (TBA-to-3HCR)...

It might be intriguing to explore the tailored ability of a bihairpin-structured molecular beacon (bhMB) for improving reaction kinetics and assay sensitivity label-free ratiometric fluorescence specific trigger (T*), in which red green silver nanoclusters (rSNC gSNC) were utilized as two reverse emitting reporters. Unlike traditional dually labeled MB, herein proposed bhMB was designed with hairpin subunits recognition signaling readout. Four functional modules included: complement T*...

Ratiometric assays of label-free dual-signaling reporters with enzyme-free amplification are intriguing yet challenging. Herein, yellow- and red-silver nanocluster (yH-AgNC rH-AgNC) acting as bicolor ratiometric emitters guided to site-specifically cluster in two template signaling hairpins (yH rH), respectively, originally, both them almost non-fluorescent. The predesigned complement tethered yH is recognizable a DNA trigger (TOC) related SARS-CoV-2. With the help an enhancer strand (G15E)...

It is intriguing to modulate the fluorescence emission of DNA-scaffolded silver nanoclusters (AgNCs) via confined strand displacement and transient concatenate ligation for amplifiable biosensing a DNA segment related SARS-CoV-2 (s2DNA). Herein, three stem-loop structural hairpins signaling, recognizing, assisting are designed assemble variant three-way device (3WDD) with aid two linkers, in which orange-emitting AgNC (oAgNC) stably clustered populated closed loop hairpin reporter. The...

Exploring the replication of hybridization chain reaction HCR (rHCR) for reciprocal amplification is intriguing in biosensing and bioanalysis. Herein, we develop a rHCR-based fluorescence platform that manipulated by combination specific DNA trigger (T) T-analogous amplicon (T*), thereby concatenating multi green-emissive Ag nanoclusters (mgAgNCs) amplifiable signal readout. Four well-designed hairpins (H1 recognizing T, H2, H3, H4) with sequential complements are executed to operate rHCR....

Exploring the ability of four-stranded DNA nanorings (

We report a ratiometric fluorescence strategy using biemissive silver nanoclusters that are harbored in functional hairpin beacon for rapid, specific and sensitive detection of HIV-related DNA as model.

Controlling the thiyl radical polymerizations is highly desirable for synthesizing polymers with precisely controlled main-chain structures yet remains challenging due to difficulty of reversibly controlling propagating radicals with...