Abstract TP53 mutations are common in lung cancers of smokers, with high prevalence G:C-to-T:A transversions generally interpreted as mutagen fingerprints tobacco smoke. In this study, (exons 5-9) and KRAS (codon 12) were analyzed primary tumors never (n = 40), former 27), current smokers 64; mainly heavy smokers). Expression p53, cyclooxygenase-2 (Cox-2), nitrotyrosine (N-Tyr), a marker protein damage by nitric oxide, immunohistochemistry. detected 47.5% never, 55.6% former, 77.4% smokers....

LAG1 is a longevity gene, the first such gene to be identified and cloned from yeast Saccharomyces cerevisiae . A close homolog of this which we call LAC1 , has been found in genome. We have human with ultimate goal examining its possible function aging. In process, also nematode worm Caenorhabditis elegans Both these homologs, LAG1Hs LAG1Ce-1 functionally complemented lethality lag1Δ lac1Δ double deletion, despite low overall sequence similarity proteins. The proteins shared short sequence,...

Platelet-activating factor (PAF) is a potent proinflammatory phospholipid with multiple pathological and physiological effects. We have shown that basic fibroblast growth (bFGF) supplementation induces rapid proliferation of human umbilical vein endothelial cells (HUVEC), which reduced upon removal bFGF or by immunoneutralization. The PAF receptor antagonist LAU-8080 inhibited bFGF-stimulated HUVEC proliferation, indicating the involvement in bFGF-mediated signaling HUVEC. Although FGF...

8-Hydroxy-2'-deoxyguanosine (8-OH-dG) is 1 of the most abundant oxidative products cellular DNA. Accumulation impaired 8-OH-dG could lead to increased genomic instability that in turn a more malignant phenotypic behavior tumors. Therefore, effects on survival 99 resected nonsmall-cell lung cancer (NSCLC) patients was evaluated.The enzyme-linked immunosorbent assay applied measure levels tumor The median were 6.5 pmol/microg for all study subjects.Patients with low had significantly longer...

Abstract A detailed cytogenetic analysis of 63 non‐small cell lung carcinomas (NSCLCs) was carried out for identification recurrent chromosomal alterations. Most specimens displayed very complex karyotypes with multiple numerical and structural changes (median number, 31). Losses chromosomes 9 (65% cases) 13 (71%) were the most frequent changes. Loss Y often observed in tumors from males. Gain chromosome 7 also (41%). Chromosome arms 1p, 1, 3p, 3q, 6q, 7q, 8q, 9p, 11q, 17p, 19q particularly...

Platelet-activating factor (PAF), a phospholipid second messenger, has diverse physiological functions, including responses in differentiated endothelial cells to external stimuli. We used human umbilical vein (HUVECs) as model system. show that PAF activated pertussis toxin-insensitive Gαq protein upon binding its seven transmembrane receptor. Elevated cAMP levels were observed via activation of adenylate cyclase, which kinase A (PKA) and was attenuated by receptor antagonist, blocking...

Tumor-induced angiogenic responses lead to complex phenotypic changes in vascular endothelial cells, which must coordinate the expression of both proteases and protease inhibitors prior proliferation invasion surrounding stroma. Matrix metalloproteinase 2 (MMP2), degrades Type IV collagen, is produced as proMMP2. proMMP2 activated part through its interactions with membrane 1 MMP (MT1-MMP) tissue inhibitor matrix (TIMP2). In this study, we demonstrate that platelet-activating factor (PAF) a...

Bombesin-like peptides (BLPs) are important regulators of lung development and may also act as autocrine growth factors in tumors. We have previously demonstrated expression mRNA for the three BLP receptor subtypes (neuromedin B [NMB] receptor, gastrin-releasing peptide [GRP] bombesin subtype 3 [BRS-3]) human non-small cell carcinoma (NSCLC) lines bronchial biopsies using reverse transcription-polymerase chain reaction (RT-PCR; DeMichele, et al. Am. J. Respir. Cell Mol. Biol. 1994;11:66–74)....

Gastrin-releasing peptide (GRP), the mammalian form of amphibian bombesin, may act as a growth-regulatory in lung. Cultured human bronchial epithelial (HBE) cells have previously been found to proliferate response GRP or bombesin. Three receptors identified for bombesin and its analogs, namely gastrin-releasing receptor (GRPR), neuromedin B (NMBR), subtype 3 (BRS-3). The genes these cloned sequenced. We used polymerase chain reaction detect transcripts three subtypes HBE cultured from...

Thyroid hormones are endocrine modulators of several vital processes that crucial to tumor growth and differentiation. Several anecdotal reports in the literature suggest some histologic types carcinoma may remain a dormant state for prolonged periods time patients with hypothyroidism, eventual progression disease once decreased thyroid function is identified corrected.Oral propylthiouracil (PTU) was used induce hypothyroidism athymic nude mice were subsequently inoculated lung...

Gastrin-releasing peptide is an important growth-modulating factor in developing lung epithelium. It known to be produced by small cell carcinomas of the lung, and autocrine loop involving gastrin-releasing its receptor has been demonstrated many tumors. We investigated whether such could also non-small carcinoma, since stimulate human bronchial epithelial cells, from which tumors should emerge. report here that a bronchiolo-alveolar carcinoma line (A549) adapted serum-free growth...

Chronic erosive gastritis (C.E.G.) is a gastric mucosal lesionwith characteristic radiological and endoscopic appearances. Pyloric gland hyperplasia seen on histological examination of biopsy specimens. C.E.G. uncommonly reported in the English literature. In reviewing 3,800 upper gastro-intestinal endoscopies from 1971--1976, 108 patients were diagnosed as having typical features chronic gastritis, an incidence 2.8%. There was significant association with duodenal ulceration overall male...

Amplification of one three growth-stimulating myc genes is a common method by which many tumor types gain proliferative advantage. In metastatic human neuroblastoma, the amplification N-myc locus, located on chromosome 2, dominant feature this usually fatal pediatric cancer. Of models proposed to explain amplification, all incorporate as initial step either disproportionate overreplication chromosomal site or recombination across loop structure. The original locus retained within in but...

To assess overexpression of the proposed tumour suppressor gene product p53 using mouse monoclonal antibody DO-7 in three main subtypes carcinoma uterine cervix and to evaluate its value as a prognostic indicator.Eighty two cases FIGO Stage IB/IIA cervical were studied retrospectively. The tumours had been previously typed into adenocarcinomas, squamous carcinomas adenosquamous after tissue fixed formalin embedded paraffin wax. protein expression was assessed standard immunohistochemical...

Abstract Cytogenetic analyses have documented the consistent deletion of part short arm chromosome 1 in neuroblastoma cells suggesting presence a suppressor gene this chromosomal region. To determine smallest region overlap at molecular level on independently derived tumors and to define location breakpoints more precisely, Southern were performed somatic cell hybrid panel containing normal altered chromosomes from seven lines. By method we able analyze 20 cloned sequences two isozymes...