A5038712683- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Melanoma and MAPK Pathways
- CAR-T cell therapy research
- Chemokine receptors and signaling
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Adhesion Molecules Research
- Influenza Virus Research Studies
- Immune Response and Inflammation
- Cutaneous Melanoma Detection and Management
- Advanced Biosensing Techniques and Applications
- bioluminescence and chemiluminescence research
- Ubiquitin and proteasome pathways
- Connective tissue disorders research
- Endodontics and Root Canal Treatments
- Dental Anxiety and Anesthesia Techniques
- Cellular Mechanics and Interactions
- Respiratory viral infections research
- Immune cells in cancer
- Proteoglycans and glycosaminoglycans research
- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Glycosylation and Glycoproteins Research
- Diffusion and Search Dynamics
University of New Mexico
2015-2024
New Mexico Cancer Center
2024
Centenary Institute
2009-2015
The University of Sydney
2014
The Wistar Institute
2006-2011
University Clinic of Traumatology
2002
Dendritic cells (DC), including those of the skin, act as sentinels for intruding microorganisms. In epidermis, DC (termed Langerhans cells, LC) are sessile and screen their microenvironment through occasional movements dendrites. The spatio-temporal orchestration antigen encounter by dermal (DDC) is not known. Since these thought to be instrumental in initiation immune responses during infection, we investigated behavior directly within natural using intravital two-photon microscopy....
The tumor microenvironment is composed of an intricate mixture and host-derived cells that engage in a continuous interplay. T are particularly important this context as they may recognize tumor-associated antigens induce regression. However, the precise identity targeted by tumor-infiltrating lymphocytes (TILs) well kinetics anatomy TIL-target cell interactions within tumors incompletely understood. Furthermore, spatiotemporal conditions TIL locomotion through stroma, prerequisite for...
Abstract The precise pathways of memory T-cell differentiation are incompletely understood. Here we exploit transgenic mice expressing fluorescent cell cycle indicators to longitudinally track the division dynamics individual CD8 + T cells. During influenza virus infection in vivo , naive cells enter a CD62L intermediate state fast proliferation, which continues for at least nine generations. At peak anti-viral immune response, subpopulation these markedly reduces their cycling speed and...
Solid cancers are composed of heterogeneous zones containing proliferating and quiescent cells. Despite considerable insight into the molecular mechanisms underlying aberrant cell cycle progression, there is limited understanding relationship between on one side, melanoma motility, invasion, drug sensitivity other side. Utilizing fluorescent ubiquitination-based indicator (FUCCI) to longitudinally monitor proliferation migration cells in 3D culture vivo, we found that invading actively,...
To better understand the initiation of CD8(+) T cell responses during infection, primary response to intracellular parasite Toxoplasma gondii was characterized using 2-photon microscopy combined with an experimental system that allowed visualization dendritic cells (DCs) and specific cells. Infection T. induced localization both these populations sub-capsular/interfollicular region draining lymph node DCs were required for expansion Consistent current models, in presence cognate antigen,...
Until recently, the main field of Er:YAG laser application was removal dental hard substances within scope cavity preparation. Nowadays, several new delivery-systems are available, permitting in endodontics. The aim present study to assess effects irradiation on root canals vitro.For this purpose, 220 extracted human teeth were endodontically processed and subsequently irradiated at different settings using an imitating vivo procedures. then subdivided into three groups subjected...
Effector T cell migration through tissues can enable control of infection or mediate inflammatory damage. Nevertheless, the molecular mechanisms that regulate effector cells within interstitial space inflamed lungs are incompletely understood. Here, we show in a mouse model acute lung injury with two-photon imaging intact tissue. Computational analysis indicates migrate an intermittent mode, switching between confined and almost straight migration, guided by lung-associated vasculature....
Vaccines that aim to expand tumor-specific CD8(+) T cells have yielded disappointing results in cancer patients although they showed efficacy transplantable tumor mouse models. Using a system more faithfully mimics progressing and its immunoinhibitory microenvironment, we here show transgenic mice, which gradually develop adenocarcinomas due expression of HPV-16 E7 within their thyroid, highly immunogenic vaccine expressing only induces low E7-specific T-cell responses, fail affect the size...
Abstract T lymphocytes utilize amoeboid migration to navigate effectively within complex microenvironments. The precise rearrangement of the actin cytoskeleton required for cellular forward propulsion is mediated by regulators, including actin‐related protein 2/3 (Arp2/3) complex, a macromolecular machine that nucleates branched filaments at leading edge. consequences modulating Arp2/3 activity on biophysical properties actomyosin cortex and downstream cell function are incompletely...
The migratory capacity of adaptive CD8αβ T cells dictates their ability to locate target and exert cytotoxicity, which is the basis immune surveillance for containment microbes disease. small intestine (SI) largest mucosal surface a primary site pathogen entrance. Using two-photon laser scanning microscopy, we found that motility antigen (Ag)-specific in SI dynamic varies with environmental milieu. Pathogen-specific cell movement differed throughout infection, becoming locally confined at...
Abstract The significant albeit limited clinical benefit of therapeutic targeting checkpoint inhibitors highlights the need for immunological understanding cancer escape from anti-tumor immunity to develop novel and better therapeutics. We surmise that many protein-protein interactions regulate T cell have yet be identified. Using human gain-of-function genetic screens, thousands proteins were screened their ability inhibit primary function. B Cell Adhesion Molecule (BCAM) was identified as...
T cells are required to clear infection, and cell motion plays a role in how quickly finds its target, from initial naive activation by dendritic interaction with target infected tissue. To better understand different tissue environments affect motility, we compared multiple features of including speed, persistence, turning angle, directionality, confinement moving murine tissues using microscopy. We quantitatively analyzed motility within the lymph node parameters activated CD8 villi small...
Abstract Effector T lymphocytes patrolling peripheral tissues protect organisms against infection and cancer. Recent two-photon imaging studies have revealed that cells move with high speed through the interstitium of inflammatory tissues. We are interested in assessing migration strategies tissue-infiltrating determining how cell motility affects efficiency target search. perform using a murine endotoxin lung model to define rules govern CD8+ effector cells. track individual moving intact...
Abstract Recruitment of effector T lymphocytes into peripheral tissues, such as inflamed lung, contributes to an effective protection against infection and cancer. The rate pathogen detection might be further influenced by the motility recruited immune cells within three-dimensional tissues. Indeed, recent two-photon studies have shown that navigate actively through lung tissue, but molecular mechanisms regulate this process are poorly characterized. Here, we used imaging a murine treated...