A5000413910- Cell death mechanisms and regulation
- Cannabis and Cannabinoid Research
- Natural product bioactivities and synthesis
- Analytical Chemistry and Chromatography
- RNA Interference and Gene Delivery
- Hepatitis B Virus Studies
- Drug Solubulity and Delivery Systems
- Pancreatic function and diabetes
- Crystallization and Solubility Studies
- Chromatography in Natural Products
- Drug Transport and Resistance Mechanisms
- Lipid metabolism and biosynthesis
- Nanoplatforms for cancer theranostics
- X-ray Diffraction in Crystallography
- Cancer-related Molecular Pathways
- Retinoids in leukemia and cellular processes
- Natural Antidiabetic Agents Studies
- Advanced Drug Delivery Systems
- Cancer, Lipids, and Metabolism
- Protein Kinase Regulation and GTPase Signaling
- Covalent Organic Framework Applications
- Cancer Research and Treatments
- Advanced Photocatalysis Techniques
- Advanced Nanomaterials in Catalysis
- Crystal structures of chemical compounds
Tianjin University
1995-2025
Nanjing University of Aeronautics and Astronautics
2023-2025
Soochow University
2024
Hyogo Medical University
2014-2022
Kagawa University
2006-2016
Mudanjiang Medical University
2016
Tianjin University of Science and Technology
2008-2016
Qiqihar Medical University
2008-2012
Anhui Normal University
1994
Abstract Ginsenosides are a group of tetracyclic triterpenoids with promising health benefits, consisting ginseng aglycone attached to various glycans. Pq3‐O‐UGT2, an important UDP‐dependent glycosyltransferase (UGT), catalyzes the production Ginsenoside Rg3 and Rd by extending glycan chain Rh2 F2, respectively, higher selectivity for F2. However, mechanism underlying its substrate recognition remains unclear. In this study, crystal structures Pq3‐O‐UGT2 in complex acceptor substrates...
N-Acylphosphatidylethanolamines (NAPEs) are precursors of bioactive N-acylethanolamines, including the endocannabinoid anandamide. In animal tissues, NAPE is formed by transfer a fatty acyl chain at sn-1 position glycerophospholipids to amino group phosphatidylethanolamine (PE), and this reaction believed be principal rate-limiting step in N-acylethanolamine synthesis. However, Ca2+-dependent, membrane-associated N-acyltransferase (NAT) responsible for has not yet been cloned. study, on...
H-Rev107 is a protein that was previously cloned as negative regulator of proto-oncogene Ras and classified class II tumor suppressor. Its structural similarity to lecithin retinol acyltransferase Ca2+-independent phosphatidylethanolamine (PE) N-acyltransferase led us analyze an enzyme involved in phospholipid metabolism. Here, we show recombinant H-Rev107s from rat, human, mouse possess phospholipase (PL) A1 or A2 activity toward phosphatidylcholine (PC). Further examination with purified...
Bioactive N-acylethanolamines (NAEs), including N-palmitoylethanolamine, N-oleoylethanolamine, and N-arachidonoylethanolamine (anandamide), are formed from membrane glycerophospholipids in animal tissues. The pathway is initiated by N-acylation of phosphatidylethanolamine to form N-acylphosphatidylethanolamine (NAPE). Despite the physiological importance this reaction, enzyme responsible, N-acyltransferase, remains molecularly uncharacterized. We recently demonstrated that all five members...
Staphylococcus aureus (S. aureus) infection has become one of the most common and severe complications among cancer patients. The impact γ radiation from radiotherapy on S. aureus's growth virulence is not yet fully understood. In this study, was exposed to at a dose 100 Gy, its descendants were cultured under normal conditions. Proteome alternations unirradiated, irradiated, irradiated identified by using data-independent acquisition (DIA) proteomic technology. To investigate consequences...
A-C1 protein is the product of a tumor suppressor gene negatively regulating oncogene Ras and belongs to HRASLS (HRAS-like suppressor) subfamily. We recently found that four members this subfamily expressed in human tissues function as phospholipid-metabolizing enzymes. Here we examined possible enzyme activity A-C1. The homogenates COS-7 cells overexpressing recombinant A-C1s from human, mouse, rat showed phospholipase A½ (PLA½) toward phosphatidylcholine (PC). This finding was confirmed...
There is accumulating evidence suggesting that tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL)‐receptor (R) 2 a promising molecular target for cancer therapy. Therefore, we investigated the effect of chemotherapeutic agents on TRAIL‐R2‐mediated apoptosis and cytotoxicity in various human solid cells. Treatment ACHN renal cell carcinoma (RCC) line with agonistic TRAIL‐R2 antibody (lexatumumab) combination 5‐fluorouracil, vinblastine, paclitaxel, or docetaxel did not overcome...
Versatile all-in-one nanoplatforms, which combine a variety of therapeutic and diagnostic capabilities, possess interesting physiochemical biological characteristics, making them highly attractive in cancer-related biomedical applications. However, up to now, the precise design nanocomposites with multiple functions has remained an ongoing challenge. Herein, this study constructed tumor microenvironment (TME)-responsive nanocomposite platform core–shell structure using bismuth (Bi) as core...
Glycyl radical enzymes (GREs) catalyze mechanistically diverse radical-mediated reactions, playing important roles in the metabolism of anaerobic bacteria. The model bacterium
TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) triggers apoptosis in various tumor cells by engaging death receptors 4 and 5. We investigated the effect of chemotherapeutic agents on receptor mediated human renal cell carcinoma using HGS-ETR1, which is a monoclonal agonistic antibody specific for 4.Cytotoxicity was determined 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Synergy assessed isobolographic analysis.Treatment ACHN line with HGS-ETR1 combined...
Two polyhedral silver-thiolate clusters, [S@Ag