A5067899791- Asthma and respiratory diseases
- IL-33, ST2, and ILC Pathways
- Extracellular vesicles in disease
- RNA modifications and cancer
- Pediatric health and respiratory diseases
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Cancer-related gene regulation
- Global Health Care Issues
- Healthcare Systems and Reforms
- Helicobacter pylori-related gastroenterology studies
- Epigenetics and DNA Methylation
- Chronic Disease Management Strategies
- Immune Response and Inflammation
- Inhalation and Respiratory Drug Delivery
- Genomics, phytochemicals, and oxidative stress
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Mast cells and histamine
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Tea Polyphenols and Effects
- Histone Deacetylase Inhibitors Research
- Protease and Inhibitor Mechanisms
- Bacillus and Francisella bacterial research
China Medical University
2011-2022
Peking University
2019-2020
King University
2019
Sheng Jing Hospital
2018
RNA N6-methyladenosine (m6A) regulators play important roles in a variety of biological functions. Nonetheless, the m6A childhood asthma remain unknown. In this study, 11 significant were selected using difference analysis between non-asthmatic and asthmatic patients from Gene Expression Omnibus GSE40888 dataset. The random forest model was used to screen five candidate (fragile X mental retardation 1, KIAA1429, Wilm’s tumor 1-associated protein, YTH domain-containing 2, zinc finger CCCH...
Previous studies have shown that curcumin alleviates asthma in vivo. However, the relationship between and nuclear factor-E2-related factor 2 (Nrf2)/haem oxygenase (HO)-1 pathway treatment remains unknown. The aim of present study was to investigate mechanisms involved amelioration airway inflammation a mouse model. Curcumin administrated asthmatic mice, bronchoalveolar lavage fluid collected. Inflammatory cell infiltration measured by Giemsa staining. Immunoglobulin E production...
Asthma is a chronic disease associated with hyperresponsiveness, obstruction and remodeling of the airways. Epithelial-mesenchymal transition (EMT) has an important role in these alterations may account for accumulation subepithelial mesenchymal cells, thus contributing to airway hyperresponsiveness remodeling. Epigallo-catechin‑3‑gallate (EGCG), which type polyphenol, most potent ingredient green tea, exhibits antibacterial, antiviral, antioxidative, anticancer chemopreventive activities....
Emerging evidence has suggested the functions of exosomes in allergic diseases including asthma. By using a mouse model with asthma induced by ovalbumin (OVA), we explored roles M2 macrophage-derived (M2Φ-Exos) progression. M2Φ-Exos significantly alleviated OVA-induced fibrosis and inflammatory responses lung tissues, as well inhibited abnormal proliferation, invasion, fibrosis-related protein production platelet derived growth factor (PDGF-BB) treated primary airway smooth muscle cells...
Asthma is usually associated with airway inflammation and remodeling. Epithelial mesenchymal transition (EMT) often occurs in The purpose of this study to identify the effect miR-21-5p Smad7 signaling pathway macrophage-derived exosomes on EMT epithelial cells.HE staining Masson were used verify successful establishment asthma model. levels cell adhesion factor stromal markers detected by Western blot. qRT-PCR. expression lung tissue was further verified fluorescence situ hybridization...
The functions of exosomes in allergic diseases including asthma have aroused increasing concerns. This paper focuses on the effects derived from human bone marrow-mesenchymal stem cells (hBM-MSCs) proliferation bronchial smooth muscle and mechanism involved. Exosomes were extracted hBM-MSCs identified. Human BSMCs induced with transforming growth factor (TGF)-β1 to mimic an asthma-like condition vitro then treated exosomes. A mouse model was by ovalbumin (OVA) for vivo study. hBM-MSC-derived...
The present study aimed to investigate the effects of vitamin D (VD) on inflammatory responses in asthmatic mice and underlying mechanism, providing a theoretical basis for clinical application targeted drug therapy, development novel drugs against asthma. Mouse models asthma were established. Hematoxylin‑eosin staining was performed observe pathological changes lung tissue. Pulmonary function tests conducted determine airway resistance mice. ELISA measure serum levels factors. Western blot...
<em>Mycoplasma pneumoniae</em> (MP) is a common pathogen that can cause respiratory infections. MP pneumonia (MPP) leads to numerous complications, including lung injury and even death. The present study aimed investigate the protective effects of Baicalin treatment on infection‑induced molecular mechanism underlying these effects. Briefly, after mice were infected intranasally by treated with (80 mg/kg), serum levels MP‑immunoglobulin M (IgM) detected ELISA. expression C‑reactive protein...
This article aimed to investigate changes in the cytoskeleton of airway smooth muscle cells (ASMCs) juvenile rats with remodeling asthma.We further investigated involvement RhoA/ROCK signaling pathway mechanism.Rat models asthma were established by antigen sensitization ovalbumin for 2, 4, 6, and 8 weeks.The control group was treated normal saline instead ovalbumin.In intervention group, after weeks culture, ASMCs ROCKspecific inhibitor Y-27632.Immunofluorescence, real-time polymerase chain...
Children exposed to common aeroallergens may develop asthma that progresses into adulthood. Inflammation regulated by T helper 2 (Th2) cells, a specific subpopulation of CD4+ lymphocytes, is involved in asthmatic injury. Herein, our microarray data indicated microRNA-451a-5p (miRNA-451a) expression decreased 4.6-fold and ETS proto-oncogene 1 (ETS1) increased 2.2-fold the peripheral blood lymphocytes isolated from children (<i>n</i> = 4) as compared control individuals 4). The...
Asthma is a chronic inflammatory disorder of the lung, which thought to be determined by balance between T helper (Th)2 and Th1 responses. This study evaluated whether Th17 cells regulatory (T reg ) was impaired in asthma patients. The proportion peripheral blood total CD4 + cell population patients significantly higher than controls (mean ± SD 0.72 0.5% versus 0.31 0.4%, respectively). lower 12.1 4.6% 27.2 7.5%, Analysis mRNA generally confirmed flow cytometry data, suggesting that changes...
Background: Chronic obstructive pulmonary disease (COPD) is the cause of substantial economic and social burden. We investigated trends in hospitalizations for acute exacerbation COPD Beijing, China, from 2009 to 2017. Patients Methods: Investigations were conducted using data discharge records inpatients that given a primary diagnosis COPD. The dataset was retrospective review information collected electronic medical included 315,116 admissions (159,368 patients). Descriptive analyses...
Abstract Background: Increasing studies have suggested that long non-coding RNAs (lncRNAs) affect the regulation of immune responses, airway inflammation, and other pathological processes asthma. In this study, we investigated functions lncRNA PTTG3P in progression childhood Methods: A competitive endogenous RNA network PTTG3P/miR-192-3p/CCNB1 was identified via bioinformatics analyses. Real-time qPCR western blot were used to quantify gene protein expression levels, respectively. Cell...
Objective To investigate the changes of levels interleukin(IL)-17 in bronchoalveolar lavage fluid (BALF) BALB/c mice infected by mycoplasma pneumonia (MP).Methods Sixtyfour were randomly divided into two groups,normal group (n =32) and MP =32).The BALF lung tissue groups collected after infection on day 3,7,14,21,each time 8.HE staining was used to observe pathological tissue.IL-17 content detected ELISA.Results The inflammatory most obvious at 7 day,and had begun subside 14 day,disappeared...
Background: China's health care reform of 2009 has brought laudable achievements, but the implications for direct economic burden respiratory diseases have not been investigated. Methods: Data from discharge records between 2008 and 2017 all patients aged over 18 with a primary diagnosis acute exacerbation chronic obstructive pulmonary disease (AECOPD) in Beijing were analyzed trends per admission capita expenditures their potential contributing factors, including age, gender, COPD type...
Abstract Background Childhood asthma is one of the most common causes hospitalization in children, causing huge economic losses worldwide. The immune system was closely linked to occurrence and development childhood asthma. main purpose this research explore role immune-related genes treatment Methods GSE40732 dataset GSE40888 were respectively regarded as training texting article. We performed weighted gene co-expression network analysis (WGCNA) select associated with based on dataset....
Abstract Type 2-pediatric asthma characterized by T2 cytokine-driven airway inflammation is the most common type of asthma. Recently, cytokine inhibitors have reduced exacerbation rates asthma, but their ability to improve lung function limited. Screening novel therapeutic strategies for patients imperative. We obtained single-cell RNA sequencing (scRNA-seq) describing chronic stimulation GSE145013 dataset with IL-13. Consensus clustering was performed classify pediatric asthmatic from...