A5015481364- Cholangiocarcinoma and Gallbladder Cancer Studies
- Pancreatic and Hepatic Oncology Research
- Gastric Cancer Management and Outcomes
- MicroRNA in disease regulation
- Gastrointestinal Tumor Research and Treatment
- Neuroendocrine Tumor Research Advances
- Radiomics and Machine Learning in Medical Imaging
- Gallbladder and Bile Duct Disorders
- Renal cell carcinoma treatment
- Lung Cancer Research Studies
- Neuroblastoma Research and Treatments
- Ubiquitin and proteasome pathways
- Renal and related cancers
- Cancer Mechanisms and Therapy
- Lung Cancer Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Genetic factors in colorectal cancer
- Cancer, Lipids, and Metabolism
- Colorectal Cancer Surgical Treatments
- Ocular Oncology and Treatments
- Gastrointestinal disorders and treatments
- Colorectal and Anal Carcinomas
- DNA Repair Mechanisms
- Lung Cancer Treatments and Mutations
- Ferroptosis and cancer prognosis
Nanjing Drum Tower Hospital
2022-2025
Shandong University
2015-2025
Qingdao University
2025
China Medical University
2018-2024
Danbury Hospital
2022-2024
University of Vermont
2022-2024
Ministry of Education of the People's Republic of China
2019-2024
Bengbu Medical College
2024
Zhongnan Hospital of Wuhan University
2023
Wuhan University
2023
MicroRNAs (miRNAs) are endogenous short (approximately 22) nucleotide RNAs that regulate gene function by modification of target mRNAs. miRNA-1 (miR-1) and miRNA-206 (miR-206) highly expressed in skeletal muscle. Due to the tissue-specific nature miR-1/206 for muscles, we investigated role development rhabdomyosarcoma. Initially, demonstrated expression was suppressed rhabdomyosarcomas found at very low levels a rhabdomyosarcoma RD cell line. Transient transfection into cultured cells led...
Reactive oxygen species (ROS) play a pivotal role in macrophage-mediated acute inflammation. However, the precise molecular mechanism by which ROS regulate macrophage polarization remains unclear. Here, we show that function as signaling molecules M1 through ataxia-telangiectasia mutated (ATM) and cell cycle checkpoint kinase 2 (Chk2), vital effector kinases DNA damage response (DDR) pathway. We further demonstrate Chk2 phosphorylates PKM2 at T95 T195 sites, promoting glycolysis facilitating...
MicroRNAs (miRNAs) are endogenously expressed, noncoding, small RNAs that inhibit protein translation through binding to target mRNAs. Recent studies have demonstrated miRNAs can regulate tumor cell proliferation and migration. MicroRNA-34a (miR-34a), a potential key effector of the p53 tumor-suppressor gene, was studied as suppressor in uveal melanoma.Northern blot analysis performed detect expression level miR-34a melanoma cells melanocytes. Subsequently, migration were examined by MTS...
MicroRNAs (miRNAs) are endogenous small non-coding RNAs that play central roles in diverse pathological processes. In this study, we investigated the effect of microRNA-182 (miR-182) on development posterior uveal melanomas. Initially, demonstrated miR-182 expression was dependent p53 induction melanoma cells. Interestingly, transient transfection into cultured cells led to a significant decrease cell growth, migration, and invasiveness. Cells transfected with cycle G1 arrest increased...
We have recently demonstrated that cytolysis of human melanoma cells by immune effectors (both NK and T cells) is associated with release the nuclear chromatin protein, high mobility group box I (HMGB1). Extracellular HMGB1 mediates a number important functions including endothelial cell activation, stromagenesis, recruitment activation innate cells, also dendritic maturation that, in setting cancer, lead to chronic inflammatory response. This reparative response promotes tumor survival,...
MicroRNAs (miRNAs) can contribute to tumorigenesis by acting as either oncogenes or tumor suppressor genes. The authors' previous studies on miR-34a showed that miRNA influence the growth of uveal melanoma cells. In this study, they investigated role miR-137 in pathogenesis melanoma.Real-time RT-PCR was used screen expression levels melanocytes and cell lines. Cell proliferation examined MTS assay cycle analyzed flow cytometry. target genes were predicted bioinformatics confirmed using a...
MicroRNA-124a (miR-124a), an abundant microRNA in the central neuron system, has been linked to tumor progression. Here, we investigated role of miR-124a uveal melanoma development.Expression cells was examined using real time RT-PCR. The effect on cell proliferation, migration, and invasion analyzed MTS assay, flow cytometry, transwell experiments. ability repress growth tested vivo. Target genes were first predicted by bioinformatics, confirmed a luciferase their expression determined...
Emerging evidence has suggested the functions of exosomes in allergic diseases including asthma. By using a mouse model with asthma induced by ovalbumin (OVA), we explored roles M2 macrophage-derived (M2Φ-Exos) progression. M2Φ-Exos significantly alleviated OVA-induced fibrosis and inflammatory responses lung tissues, as well inhibited abnormal proliferation, invasion, fibrosis-related protein production platelet derived growth factor (PDGF-BB) treated primary airway smooth muscle cells...
Retinal pigment epithelial (RPE) cells play important roles in ophthalmologic diseases such as proliferative vitreoretinopathy, AMD, and diabetic retinopathy. MicroRNA-34a (miR-34a) has been reported to be the regulation of cell proliferation, migration, differentiation, apoptosis. In this study, we explored effects miR-34a on RPE cells.The expression level subconfluent postconfluent ARPE-19 was investigated with quantitative real-time PCR. MicroRNA mimic small interfering RNA (siRNA) were...
Abstract Oncogenic stress induces DNA damage repair (DDR) that permits escape from mitotic catastrophe and allows early precursor lesions during the evolution of cancer. SAMHD1, a dNTPase protecting cells viral infections, has been recently found to participate in process. However, its role tumorigenesis remains largely unknown. Here, we show SAMHD1 is up-regulated early-stage human carcinoma tissues cell lines under oxidative or genotoxic insults. We further demonstrate de-ubiquitinating...
TFE3 rearranged renal cell carcinoma (TFE3 rRCC), classified as a distinct entity of RCCs, exhibits aggressive biological behavior and possesses unique metabolic characteristics. In the present study, rRCC with high expression fusion proteins was employed to investigate features lipid metabolism its underlying mechanism in cancer progression. Fluorescence microscope flow cytometry were detect droplets (LDs). GPO-PAP method Oil Red O staining used quantify triacylglycerol levels. The data for...
Most Muslim and Jewish Mutakallimūn accepted the definition of necessary knowledge as opposed to inferential knowledge, with one remarkable exception, namely, Saadya’s problematic use this term. He characterized some type mediate “necessary knowledge” accordingly introduced a second-order that is necessarily concomitant original one. This move may have marked synthesis two main epistemological trends (classical intellectualism analytical empiricism) at time.
Proliferative vitreoretinopathy (PVR) is the most serious fibrous complication that causes vision loss after intraocular surgery, and there currently no effective treatment in clinical.Autophagy an important cell biological mechanism maintaining homeostasis of tissues cells, resisting process EMT.However, it still unclear whether autophagy could resist fibrosis prevent PVR progression.In this study, we investigated expression mesenchymal biomarkers deficiency cells found these proteins were...
Metabolic reprogramming is a distinct hallmark in tumorigenesis. Autophagy can rewire cell metabolism by regulating intracellular homeostasis. Warburg effect specific energy metabolic process that allows tumor cells to metabolize glucose via glycolysis into lactate even the presence of oxygen. Although both autophagy and are involved stress response crisis cells, their molecular relationship has remained largely elusive. We found Atg7, key molecule autophagy, inhibits effect....
purpose. Eyelid development is a dynamic process involving cell proliferation, differentiation, and migration regulated by number of growth factors cytokines. Mice deficient in the orphan G protein–coupled receptor 48 (GPR48) showed an eye open at birth (EOB) phenotype. In this study, authors attempted to clarify role GPR48 eyelid molecular mechanisms leading EOB methods. Phenotypic analysis eyelids Gpr48 −/− mice was carried out using histology scanning electron microscopy. expression...
MicroRNAs are endogenous short chain nucleotide RNAs that regulate gene function by direct binding of target mRNAs. In this study, we investigated the effects microRNA-206 (miR-206) on development gastric cancer. miR-206 was first confirmed to be downregulated in cancer specimens. Conversely, upregulation c-Met tissue samples human cancer, with its level inversely correlated expression. Introduction inhibited cellular proliferation inducing G1 cell cycle arrest, as well migration and...
DNA damage signals transducer RING finger protein 8 (RNF8) is involved in maintaining genomic stability by facilitating the repair of double-strand breaks (DSB) via ubiquitin signaling. By analyzing TCGA database and colon cancer tissue microarrays, we found that expression level RNF8 was positively correlated with c-Myc cancer, which were closely associated poor survival patients. Furthermore, overexpressing knocking down increased decreased cells, respectively. In addition, interacted...
GPR48 can mediate keratinocyte proliferation and migration. Our investigations showed that AG1478, an inhibitor of EGFR tyrosine kinase, could block GPR48‐mediated cellular processes. AG1478 treatment Gpr48 +/+ cells also decreased phosphorylation EGFR, ERK STAT3. Subsequent screening using conditioned media immunodepleted ligands identified HB‐EGF as the ligand responsible for was reduced in −/− cell culture medium, but its addition restored ERK, STAT3, well proliferation. Confirmation...
Accumulating evidence suggests that improvements in osteogenesis and angiogenesis play an important role repairing osteoporotic bone defects. Cinnamomum cassia (C. cassia), a traditional Chinese medicinal herb, is reported to show anabolic effects on osteoblasts. However, whether C. could actually repair defects conditions remains unknown. The purpose of this study was evaluate the effect combined treatment with Cinnamaldehyde (main oil isolated from cassia) β-tricalcium phosphate (β-TCP)...
Purpose: MicroRNA-34a (miR-34a) has been implicated in many biological processes. It is downregulated uveal melanoma, and introduction of miR-34a inhibits the proliferation migration melanoma cells. Leucine-rich repeat-containing G protein-coupled receptor 4 (LGR4) a novel target identified first retinal pigment epithelial In this study, we sought to evaluate interaction LGR4 its downstream mechanisms. Methods: The expression LGR4, epithelial–mesenchymal transition (EMT)-associated factors,...