A5031501193- Malaria Research and Control
- Mosquito-borne diseases and control
- Complement system in diseases
- Vector-borne infectious diseases
- Sarcoma Diagnosis and Treatment
- Virus-based gene therapy research
- Cancer Research and Treatments
- Viral Infectious Diseases and Gene Expression in Insects
- Healthcare and Venom Research
- Parasites and Host Interactions
- Evolution and Genetic Dynamics
- CAR-T cell therapy research
- Insect Pest Control Strategies
- Protein Degradation and Inhibitors
- Invertebrate Immune Response Mechanisms
- Cell Image Analysis Techniques
- Cancer-related gene regulation
Radboud University Nijmegen
2013-2023
Radboud University Medical Center
2013-2023
University Medical Center
2023
Radboud Institute for Molecular Life Sciences
2022
Lunenfeld-Tanenbaum Research Institute
2014-2015
Mount Sinai Hospital
2015
The Netherlands Cancer Institute
1994
The malaria parasite life cycle includes asexual replication in human blood, with a proportion of parasites differentiating to gametocytes required for transmission mosquitoes. Commitment differentiate into gametocytes, which is marked by activation the transcription factor ap2-g , known be influenced host factors but comprehensive model remains uncertain. Here, we analyze data from 828 children Kilifi, Kenya severe, uncomplicated, and asymptomatic infection over 18 years falling...
For malaria elimination efforts, it is important to better understand parasite transmission mosquitoes and develop models for early-clinical evaluation of transmission-blocking interventions.In a randomized open-label trial, 24 participants were infected by bites from Plasmodium falciparum 3D7-infected (mosquito bite [MB]; n = 12) or induced blood-stage (IBSM) with the same line (n 12). After subcurative piperaquine treatment, asexual gametocytes kinetics assessed, mosquito feeding...
BackgroundFever and inflammation are a hallmark of clinical Plasmodium falciparum (Pf) malaria induced by circulating asexual parasites. Although manifestations associated with parasite density, this relationship is influenced complex network immune-modulating factors both human origin.MethodsIn the Controlled Human Malaria infection (CHMI) model, we compared in healthy malaria-naïve volunteers infected either Pf-infected mosquito bites (MB, n=12) or intravenous administration red blood...
Cancer is a complex process in which normal cell has to accumulate multiple genetic and epigenetic alterations order become highly malignant metastasizing tumor cell. Over the years, efforts have concentrated on identifying sequence of events leading phenotype cells. Our understanding this steadily growing with identification an increasing number genes that are frequently modified tumors characterization their role specific processes proliferation transformation. During last decade,...
// Timothy McKinnon 1 , Rosemarie Venier Brendan C. Dickson 4 Leah Kabaroff Manon Alkema Li Chen 5 Jack F. Shern Marielle E. Yohe Javed Khan Rebecca A. Gladdy 1, 2, 3 Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Canada 2 Ontario Institute for Cancer Research, Stem Cell Program, Department of Surgery, University Pathology & Laboratory Medicine, Genetics Branch, Oncogenomics Section, Center National Health, Gaithersburg, MD, USA Correspondence to: Gladdy, e-mail:...
Individuals infected with P. falciparum develop antibody responses to intra-erythrocytic gametocyte proteins and exported present on the surface of erythrocytes. However, there is currently limited knowledge immunogenicity antigens specificity gametocyte-induced responses. In this study, we assessed in participants two controlled human malaria infection (CHMI) studies by ELISA, multiplexed bead-based assays protein microarray. By comparing without exposure, aimed disentangle response induced...
Abstract Background Whole sporozoite immunization under chemoprophylaxis (CPS regime) induces long-lasting sterile homologous protection in the controlled human malaria infection model using Plasmodium falciparum strain NF54. The relative proficiency of liver-stage parasite development may be an important factor determining efficacy. Previous studies show that NF135 produces relatively high numbers large schizonts vitro. Here, we evaluate this for use CPS regimes. Methods In a partially...
Malaria transmission depends on the presence of mature Plasmodium stages (gametocytes) that may render blood-feeding Anopheles mosquitos infectious. Transmission-blocking antimalarial drugs and vaccines can prevent by reducing gametocyte densities or infectivity to mosquitos. Mosquito infection outcomes are thereby informative biological endpoints clinical trials with blocking interventions. Nevertheless, often primarily designed determine intervention safety; efficacy is difficult...
Direct membrane feeding assays assess the transmission potential of malaria-infected individuals using whole blood collected in anticoagulant vacutainers.The inhibitory effect four commonly used anticoagulants on gametocyte infectivity to mosquitoes was assessed standard with cultured Plasmodium falciparum.Infection burden significantly reduced when sodium citrate and EDTA. Transmission highest lithium heparin heparin, although a concentration-dependent inhibition mosquito infection also...
Abstract Introduction: Rhabdomyosarcoma (RMS) represents the most common pediatric soft tissue sarcoma. Despite advances in multimodality therapy, outcomes intermediate and high-risk RMS groups have plateaued. To develop more efficacious therapies, improved biologic models of are required to better characterize molecular pathogenesis RMS. Results: We developed a mouse model that targets maturing myoblasts, likely cell-of-origin for In this study, myoblasts were isolated from neonatal...
Abstract Animal models are a powerful means to dissect the molecular events regulating carcinogenesis and also serve as pre-clinical for drug development. Rhabdomyosarcoma (RMS) is amongst most common of pediatric sarcomas whose dismal outcome in high-risk groups has unfortunately not improved past decade. Current animal RMS include zebrafish - an elegant genetic system that many advantages such rapid generation mutants ease live imaging, but may faithfully recapitulate sarcomagenesis...
Abstract The malaria parasite life cycle includes asexual replication in human blood, with a proportion of parasites differentiating to gametocytes required for transmission mosquitoes. Commitment differentiate into gametocytes, which is marked by activation the transcription factor ap2-g , known be influenced host factors but comprehensive model remains uncertain. Here we analyze data from 828 children Kilifi, Kenya severe, uncomplicated, and asymptomatic infection over 18 years falling...