A5079165156- Plant Molecular Biology Research
- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Plant and animal studies
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Influenza Virus Research Studies
- Light effects on plants
- Viral gastroenteritis research and epidemiology
- Hepatitis B Virus Studies
- Animal Disease Management and Epidemiology
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Lipid metabolism and biosynthesis
- Plant Surface Properties and Treatments
- Genetic Mapping and Diversity in Plants and Animals
- Microtubule and mitosis dynamics
- Quantum Dots Synthesis And Properties
- Genetic and Environmental Crop Studies
- Animal Virus Infections Studies
University of Georgia
2023-2024
Shanghai Jiao Tong University
2016-2022
Anhui Agricultural University
2020-2022
Chinese Academy of Agricultural Sciences
2009-2021
University of California, Berkeley
2021
Northwest A&F University
2018-2021
The University of Adelaide
2020
Sichuan Agricultural University
2019
Jiangsu Academy of Agricultural Sciences
2018
Shanghai Veterinary Research Institute
2009-2016
RNA interference (RNAi) caused by exogenous double-stranded (dsRNA) has developed into a powerful technique in functional genomics, and to date it is widely used down-regulate crucial physiology-related genes control pest insects. A molt-regulating transcription factor gene, HaHR3, of cotton bollworm (Helicoverpa armigera) was selected as the target gene. Four different fragments covering coding sequence (CDS) HaHR3 were cloned vector L4440 express dsRNAs Escherichia coli. The most effective...
Aliphatic and aromatic lipids are both essential structural components of the plant cuticle, an important interface between environment. Although cross links aliphatic or other moieties known to be associated with formation leaf cutin root seed suberin, contribution biosynthesis anther cuticles pollen walls remains elusive. In this study, we characterized rice (Oryza sativa) male sterile mutant, defective wall 2 (dpw2), which showed abnormal a wall, complete sterility. Compared wild type,...
Red (R) and far-red (FR) light distinctly influence phytochrome-mediated initial tomato growth development, more recent evidence indicates that these spectra also modulate responses to a multitude of abiotic biotic stresses. This research investigated whether different R: FR values affect response salinity tolerance. Tomato seedlings were exposed conditions (7.4, 1.2 0.8) under stress (100 mM NaCl), evaluated for their growth, biochemical changes, active reactive oxygen species (ROS) ROS...
H9N2 avian influenza virus (AIV) has an extended host range, but the molecular basis underlying AIV transmission to mammals remains unclear. We isolated more than 900 AIVs in our 3-year surveillance live bird markets China from 2009 2012. Thirty-seven representative isolates were selected for further detailed characterization. These categorized into 8 genotypes (B64 B71) and formed a distinct antigenic subgroup. Three belonging genotype B69, which is predominant circulating China, replicated...
RNA interference, which involves the delivery of small interfering (siRNA), has been used to validate target genes, understand and control cellular metabolic pathways, use as a "green" alternative confer pest tolerance in crops. Conventional siRNA methods such viruses Agrobacterium-mediated exhibit plant species range limitations uncontrolled DNA integration into genome. Here, we synthesize polyethylenimine-functionalized gold nanoclusters (PEI-AuNCs) mediate intact plants show that these...
During pollen wall formation in flowering plants, a conserved metabolon consisting of acyl-CoA synthetase (ACOS), polyketide synthase (PKS) and tetraketide α-pyrone reductase (TKPR), is required for sporopollenin synthesis. Despite this, the precise function each these components different species remains unclear.In this study, we characterized OsTKPR1, rice orthologue Arabidopsis TKPR1. Loss OsTKPR1 delayed tapetum degradation, reduced levels anther cuticular lipids, impaired Ubisch body...
The timely programmed cell death (PCD) of the tapetum, innermost somatic anther layer in flowering plants, is critical for pollen development, including deposition and patterning wall. Although several genes involved tapetal PCD wall development have been characterized, underlying regulatory mechanism remains elusive. Here we report that PERSISTENT TAPETAL CELL2 (PTC2), which encodes an AT-hook nuclear localized protein rice (Oryza sativa), required normal development. mutant ptc2 showed...
In flowering plants, successful male reproduction requires the sophisticated interaction between somatic anther wall layers and reproductive cells. Timely degradation of innermost tissue layer, tapetal is critical for pollen development. Ca2+ a well-known stimulus plant development, but whether it plays role in affecting remains elusive. Here we report Defective Exine Formation 1 (OsDEX1) rice (Oryza sativa), binding protein, regulating cell formation. osdex1 anthers, degeneration delayed...
Abstract Lipid and phenolic metabolism are important for pollen exine formation. In Arabidopsis , polyketide synthases (PKSs) essential both sporopollenin biosynthesis Here, we characterized the role of a synthase (OsPKS2) in male reproduction rice ( Oryza sativa ). Recombinant OsPKS2 catalyzed condensation fatty acyl‐CoA with malonyl‐CoA to generate triketide tetraketide α‐pyrones, main components exine. Indeed, ospks2 mutant had defective patterning was sterile. However, showed no...
Summary In flowering plants, pollen wall is a specialized extracellular cell‐wall matrix surrounding male gametophytes and acts as natural protector of grains against various environmental biological stresses. The formation complex but well‐regulated process, which involves the action many different genes. However, genetic molecular mechanisms underlying this process remain largely unknown. study, we isolated characterized novel rice sterile mutant, defective wall3 ( dpw3 ), displays smaller...
Glycerolipids are essential for rice development and grain quality but its genetic regulation remains unknown. Here we report base using metabolite-based genome-wide association study quantitative traits locus (QTL) analyses based on lipidomic profiles of seeds from 587 Asian cultivated accessions 103 chromosomal segment substitution lines, respectively. We found that two genes encoding phosphatidylcholine (PC):diacylglycerol cholinephosphotransferase (OsLP1) granule-bound starch synthase I...
Plants can regenerate new organs from damaged or detached tissues. In the process of de novo root regeneration (DNRR), adventitious roots are frequently formed wound site on a leaf. Salicylic acid (SA) is key phytohormone regulating plant defenses and stress responses. The role SA its acting mechanisms during organogenesis still unclear. Here, we found that endogenous inhibited formation after cutting. Free rapidly accumulated at site, which was accompanied by an activation response....
To investigate whether the high expression levels of actin-depolymerizing factor genes are related to pollen development, three GhADF (cDNAs) were isolated and characterized in cotton. Among them, GhADF6 GhADF8 preferentially expressed petals, whereas GhADF7 displayed highest level anthers, revealing its anther specificity. The transcripts anthers reached peak value at flowering, suggesting that is developmentally-regulated anthers. gene including promoter region was from cotton genome....
Abstract This article comments on: Wu Y, Sexton WK, Zhang Q, Bloodgood D, Hooks C, Coker F, Vasquez A, Wei C-I, Xiao S. 2024. Leaf abaxial immunity to powdery mildew in Arabidopsis is conferred by multiple defense mechanisms. Journal of Experimental Botany 75, 1465–1478.
Photoperiod is a crucial inducer of plant flowering. Cycling DOF factors (CDFs) play pivotal roles in the flowering long-day (LD) and short-day (SD) plants. However, functions CDFs photoperiod regulated remain unclear day-neutral In present study, tomato ( Solanum lycopersicum L. cv. “Ailsa Craig”) seedlings wild-type transgenic lines overexpressing were treated with different photoperiods. The time expression pattern SlCDFs other FT-like genes investigated. results showed that SlCDF1 ,...
Currently, both goose astrovirus (GoAstV) and goose-origin Newcastle disease virus (NDV) are widely infectious agents for goslings. There is no vaccine GoAstV. Capsid protein can elicit a neutralizing antibody in human astroviruses (HAstV). Molecular analysis of the genomic region encoding capsid protein(ORF2) has revealed that it contains epitopes. Goose-origin NDV also an agent. A wide range strains exist be commonly used as vectors. In present study, fusion cleavage site RRQKR↓F backbone...
Phytochromes are dimeric proteins with critical roles in perceiving day length and the environmental signals that trigger flowering. Night break (NB) red to far-red light ratio (R:FR) have been used extensively as tools study photoperiodic control of However, at molecular level, little is known about effect NB different R:FR values on flowering day-neutral plants (DNPs) such tomato. Here, we show tomato SP5G, SP5G2, SP5G3 homologs Arabidopsis thaliana FLOWERING LOCUS T (FT) repress Nicotiana...
SUMMARY The phytohormone cytokinin plays a significant role in nearly all aspects of plant growth and development. Cytokinin signaling has primarily been studied the dicot model Arabidopsis, with relatively little work done monocots, which include rice ( Oryza sativa ) other cereals agronomic importance. pathway is phosphorelay comprised histidine kinase receptors, authentic phosphotransfer proteins (AHPs) type‐B response regulators (RRs). Two negative have identified: type‐A RRs, are...
Abstract BAHD superfamily acyltransferases play an important role in catalyzing and regulating secondary metabolism plants. Despite this, there is relatively little information regarding the barley. In this study, we identified 116 HvBAHD from barley genome. Based on phylogenetic analysis classification model monocotyledonous dicotyledonous plants, divided genes into eight groups, I-a, I-b, II, III-a, III-b, IV, V-a V-b. The Clade IV genes, including Agmatine Coumarol Transferase ( ACT )...
Kinesin, as a member of the molecular motor protein superfamily, plays an essential function in various plants’ developmental processes. Especially at early stages plant growth, including influences on growth rate, yield, and quality. In this study, we did genome-wide identification expression profile analysis kinesin family barley. Forty-two HvKINs were identified screened from barley genome, generated phylogenetic tree was used to compare evolutionary relationships between Rice...
ABSTRACT De novo root regeneration (DNRR) is a developmental process that regenerates adventitious roots from wounded tissues. Phytohormone signaling pathways involved in microbial resistance are mobilized after cutting and influence de regeneration. Microbes may positively or negatively the development stress responses of plant. However, most studies on molecular mechanisms organogenesis performed aseptic conditions. Thus, potential crosstalk between organ biotic stresses underexplored....